Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A32 F30 I1 R1	30	51.0	1723162	99.3%	1711099	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,363,183	G→T	P151T (CCC→ACC)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,363,183	0	G	T	100.0%	83.6 / NA	25	P151T (CCC→ACC)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (10/15); total (10/15)

GTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACG  >  NC_000913/2363048‑2363315

gtgCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGgtagta                                                                                                                                  >  2:733586/1‑140 (MQ=255)

gtgCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGgtagta                                                                                                                                  >  2:723736/1‑140 (MQ=255)

       aTCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACa                                                                                                                           >  2:96626/1‑140 (MQ=255)

             ttCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGc                                                                                                                     <  2:388172/140‑1 (MQ=255)

                                 tCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCa                                                                                                 <  1:300009/140‑1 (MQ=255)

                                           aCAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAgg                                                                                       <  1:723736/140‑1 (MQ=255)

                                                    cggcTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTa                                                                              <  1:373880/140‑1 (MQ=255)

                                                     ggcTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACAGTACGCCGGTAGTAAAGAACATCAGGCAGCGATTAAGCTGCTCGTCAAATCCCCAGTAGGTGCATat                                                                             <  2:590413/140‑2 (MQ=255)

                                                          cGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGt                                                                        >  1:109356/1‑140 (MQ=255)

                                                          cGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGt                                                                        <  1:230084/140‑1 (MQ=255)

                                                                         cTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAg                                                         >  1:569300/1‑140 (MQ=255)

                                                                               cAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTgg                                                   <  1:604657/140‑1 (MQ=255)

                                                                                        cAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCt                                          >  1:776238/1‑140 (MQ=255)

                                                                                        cAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCt                                          >  1:367420/1‑140 (MQ=255)

                                                                                            aTTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGc                                      <  1:286663/140‑1 (MQ=255)

                                                                                                 gTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGtt                                 <  2:776238/140‑1 (MQ=255)

                                                                                                 gTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGtt                                 <  2:367420/140‑1 (MQ=255)

                                                                                                   gCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTa                               <  2:569300/140‑1 (MQ=255)

                                                                                                             cACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAATCCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCg                     >  1:782281/1‑140 (MQ=255)

                                                                                                             cACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCg                     <  1:96626/140‑1 (MQ=255)

                                                                                                                  tAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGcc                <  1:733586/140‑1 (MQ=255)

                                                                                                                   aaaTTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTc                      >  2:421365/1‑133 (MQ=255)

                                                                                                                   aaaTTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTc                      <  1:421365/133‑1 (MQ=255)

                                                                                                                   aaaTTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCa               >  1:792264/1‑140 (MQ=255)

                                                                                                                          gACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCg        >  1:143328/1‑140 (MQ=255)

                                                                                                                          gACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCg        >  1:737493/1‑140 (MQ=255)

                                                                                                                              gTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCa    <  2:96899/140‑1 (MQ=255)

                                                                                                                                aCGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACg  <  2:109356/140‑1 (MQ=255)

GTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACG  >  NC_000913/2363048‑2363315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A