Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A32 F30 I1 R1	30	51.0	1723162	99.3%	1711099	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,505,167	C→A	G370G (GGC→GGA)	yjhB →	putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,505,167	0	C	A	100.0%	206.7 / NA	59	G370G (GGC→GGA)	yjhB	putative sialic acid transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (36/23); total (36/23)

GGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGT  >  NC_000913/4505035‑4505303

ggttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgtg                                                                                                                                   <  2:375224/140‑1 (MQ=255)

 gttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgtgt                                                                                                                                  >  1:101434/1‑140 (MQ=255)

 gttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgtg                                                                                                                                   <  2:709947/139‑1 (MQ=255)

 gttCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgtg                                                                                                                                   >  1:709947/1‑139 (MQ=255)

 gttCCAAAATTTATATATGACTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGgggt                                                                                                                                  >  2:256480/1‑140 (MQ=255)

          tttATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCattatt                                                                                                                         <  2:101434/140‑1 (MQ=255)

                  tGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 <  2:422699/140‑1 (MQ=255)

                  tGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 >  1:422699/1‑140 (MQ=255)

                    aTTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACg                                                                                                               >  1:132424/1‑140 (MQ=255)

                     ttACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 <  1:764781/137‑1 (MQ=255)

                     ttACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTa                                                                                                                 >  2:764781/1‑137 (MQ=255)

                            ccAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCAtt                                                                                                       >  2:590286/1‑140 (MQ=255)

                             caacaaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATtc                                                                                                      >  1:329117/1‑140 (MQ=255)

                             caacaaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATtc                                                                                                      >  1:564742/1‑140 (MQ=255)

                                caaAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTctct                                                                                                   <  2:35631/140‑1 (MQ=255)

                                    aTTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCt                                                                                               <  2:513633/140‑1 (MQ=255)

                                             aTTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAAttt                                                                                      >  1:772044/1‑140 (MQ=255)

                                              ttAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAAtttt                                                                                     >  1:94868/1‑140 (MQ=255)

                                                      cGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTg                                                                             >  2:370395/1‑140 (MQ=255)

                                                      cGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTCCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTg                                                                             >  2:670238/1‑140 (MQ=255)

                                                       ggTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTgg                                                                            >  1:270697/1‑140 (MQ=255)

                                                       ggTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTgg                                                                            >  1:332603/1‑140 (MQ=255)

                                                                 ataACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATAtt                                                                  <  2:270697/140‑1 (MQ=255)

                                                                  taACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTc                                                                 >  1:465855/1‑140 (MQ=255)

                                                                   aaCTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTcc                                                                >  1:72182/1‑140 (MQ=255)

                                                                   aaCTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTcc                                                                >  1:813260/1‑140 (MQ=255)

                                                                   aaCTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTcc                                                                >  2:542839/1‑140 (MQ=255)

                                                                        aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                           >  2:194165/1‑140 (MQ=255)

                                                                        aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                           >  1:662828/1‑140 (MQ=255)

                                                                        aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                           >  1:204695/1‑140 (MQ=255)

                                                                        aGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTa                                                           >  2:521007/1‑140 (MQ=255)

                                                                          gggCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTaaa                                                         >  1:433339/1‑140 (MQ=255)

                                                                             cAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAAtt                                                      <  1:194165/140‑1 (MQ=255)

                                                                                cTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTtat                                                   >  2:93899/1‑140 (MQ=255)

                                                                                  ggaggaATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATaa                                                 <  2:236147/140‑1 (MQ=255)

                                                                                    aggaATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAAc                                               >  2:451267/1‑140 (MQ=255)

                                                                                    aggaATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAAc                                               >  2:685813/1‑140 (MQ=255)

                                                                                          ggCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCg                                         <  1:93899/140‑1 (MQ=255)

                                                                                                  cTGTATTAGCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTaaa                                 <  2:329117/140‑1 (MQ=255)

                                                                                                          gCTACATACATTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGa                         <  2:74031/140‑1 (MQ=255)

                                                                                                            tacatacaTTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGAt                             <  2:339400/134‑1 (MQ=255)

                                                                                                            tacatacaTTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGAt                             >  1:339400/1‑134 (MQ=255)

                                                                                                              catacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCt                     <  1:542839/140‑1 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTATGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATATACTATCCGTGGCTAAATGATTGGAGGCtt                    >  2:727921/1‑140 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGa                         <  1:549329/135‑1 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGa                         >  2:549329/1‑135 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  2:792531/1‑140 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  1:115091/1‑140 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    <  2:564742/140‑1 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  2:523364/1‑140 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  2:495702/1‑140 (MQ=255)

                                                                                                               atacatTTCAGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCtt                    >  1:386006/1‑140 (MQ=255)

                                                                                                                        aGGATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGattaa           >  2:129090/1‑140 (MQ=255)

                                                                                                                         ggATATTATGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGattaat          >  1:334083/1‑140 (MQ=255)

                                                                                                                           atatTATGGATTAGGTGTTTCATTATTCATTGTTATGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATATACTATCCGTGGCTAAATGATTGGAGGCTTTATGattaatta        <  1:727921/140‑1 (MQ=255)

                                                                                                                            tattatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTAt       <  1:444891/140‑1 (MQ=255)

                                                                                                                             attatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATg      <  2:72182/140‑1 (MQ=255)

                                                                                                                             attatGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATg      <  1:521007/140‑1 (MQ=255)

                                                                                                                             attatGGATTAGGTGGTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATg      >  2:625191/1‑140 (MQ=255)

                                                                                                                                atGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCg   <  2:132424/140‑1 (MQ=255)

                                                                                                                                 tGGATTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCgt  <  1:685813/140‑1 (MQ=255)

GGTTCCAAAATTTATATATGATTACTTTCCAACAAAATTAAGAGGATTAGGGACCGGTCTTATTTATAACTTAGGGGCAACTGGAGGAATGGCCGCACCTGTATTAGCTACATACATTTCAGGATATTATGGCTTAGGTGTTTCATTATTCATTGTTACGGTTGCATTCTCTGCCTTATTAATTTTGTTAGTTGGTTTTGATATTCCAGGTAAAATTTATAAACTATCCGTGGCTAAATGATTGGAGGCTTTATGATTAATTATGGCGT  >  NC_000913/4505035‑4505303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A