Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A25 F30 I1 R1	18	41.0	1418494	98.5%	1397216	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,345	C→A	intergenic (‑63/+28)	prfB ← / ← recJ	peptide chain release factor RF2/ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,345	0	C	A	100.0%	152.6 / NA	44	intergenic (‑63/+28)	prfB/recJ	peptide chain release factor RF2/ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (22/22); total (22/22)

AGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTAT  >  NC_000913/3036209‑3036476

aGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACg                                                                                                                                  <  2:242271/140‑1 (MQ=255)

aGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACg                                                                                                                                  <  1:297990/140‑1 (MQ=255)

  aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  2:205045/140‑1 (MQ=255)

  aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  1:74215/140‑1 (MQ=255)

  aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  1:646195/140‑1 (MQ=255)

  aTACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGct                                                                                                                                <  1:578907/140‑1 (MQ=255)

     cccccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtt                                                                                                                             >  2:586436/1‑140 (MQ=255)

      ccccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCttt                                                                                                                            >  1:170914/1‑140 (MQ=255)

      ccccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCttt                                                                                                                            >  2:501757/1‑140 (MQ=255)

        ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtttt                                                                                                                           <  1:228339/139‑1 (MQ=255)

        ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtttt                                                                                                                           >  2:228339/1‑139 (MQ=255)

        ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtt                                                                                                                             <  1:317009/137‑1 (MQ=255)

        ccTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCtt                                                                                                                             >  2:317009/1‑137 (MQ=255)

                  tCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGagaag                                                                                                                >  2:451714/1‑140 (MQ=255)

                        cGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACg                                                                                                          <  1:533164/140‑1 (MQ=255)

                                     ccTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAg                                                                                             >  1:375326/1‑140 (MQ=255)

                                      cTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGa                                                                                            >  1:78178/1‑140 (MQ=255)

                                       tGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGat                                                                                           >  1:251902/1‑140 (MQ=255)

                                              gATTATTTACCGGATTAATTTCAACCATGGTCTGATTTCTTTTATTGAGCTAGTC‑AAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  2:443349/140‑1 (MQ=255)

                                               attattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  1:586436/140‑1 (MQ=255)

                                               attattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  2:375326/140‑1 (MQ=255)

                                               attattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCga                                                                                   <  2:78178/140‑1 (MQ=255)

                                                 tattTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCgatg                                                                                 >  2:43161/1‑140 (MQ=255)

                                                          gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGata                                                                                          <  1:193029/122‑1 (MQ=255)

                                                          gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGata                                                                                          >  2:193029/1‑122 (MQ=255)

                                                          gATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGc                                                                        >  2:650558/1‑140 (MQ=255)

                                                             tAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAgg                                                                     >  1:44483/1‑140 (MQ=255)

                                                                              tGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGaaa                                                    <  2:44483/140‑1 (MQ=255)

                                                                                      tttATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGa                                            >  2:601855/1‑140 (MQ=255)

                                                                                                  gTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTtat                                >  1:96069/1‑140 (MQ=255)

                                                                                                         tGCGGTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAg                         >  2:14608/1‑140 (MQ=255)

                                                                                                            ggTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATaa                              <  2:3779/132‑1 (MQ=255)

                                                                                                            ggTGATAAGAGCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATaa                              >  1:3779/1‑132 (MQ=255)

                                                                                                                      gCGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGGTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc            <  1:601855/140‑1 (MQ=255)

                                                                                                                       cGGGATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa           <  2:170914/140‑1 (MQ=255)

                                                                                                                        gggATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCAc          >  1:311448/1‑140 (MQ=255)

                                                                                                                         ggATTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACg         >  2:365853/1‑140 (MQ=255)

                                                                                                                           aTTGTACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGcc       <  1:451714/140‑1 (MQ=255)

                                                                                                                               tACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc            >  1:21182/1‑131 (MQ=255)

                                                                                                                               tACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCgcgc            <  2:21182/131‑1 (MQ=255)

                                                                                                                               tACCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTa   >  2:173377/1‑140 (MQ=255)

                                                                                                                                aCCCAATCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTAt  <  2:59231/140‑1 (MQ=255)

                                                                                                                                    aaTCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa           >  1:632079/1‑127 (MQ=255)

                                                                                                                                    aaTCAACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCa           <  2:632079/127‑1 (MQ=255)

AGATACCCCCTAAGAACGTCGGAGCGTTCCGTGAGGTCCTGAATGCGATTATTTACCGGATTAATTTCAAACATGGTCTGATTTCTTTTATTGAGCTAGTCAAAATGCGGTGATAAGAGCGGGATTGTACCCAATCCACGCTCTTTTTTATAGAGAAGATGACGCTAAATTGGCCAGATATTGTCGATGATAATTTGCAGGCTGCGGTTGCCGCGAAACTCGTTGATATCGAGCTTATAAGCCAGTTGCACTTCGCGCACGCCGTTAT  >  NC_000913/3036209‑3036476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A