Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F30 I1 R1	19	47.3	1605240	99.2%	1592398	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,703,667	C→A	G61V (GGC→GTC)	dppD ←	dipeptide ABC transporter ATP binding subunit DppD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,703,667	0	C	A	100.0%	124.0 / NA	36	G61V (GGC→GTC)	dppD	dipeptide ABC transporter ATP binding subunit DppD
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (18/18); total (18/18)

AGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGGCGCGGAACGGCGCGCTTTC  >  NC_000913/3703533‑3703803

aGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGa                                                                                                                                     >  2:230389/1‑140 (MQ=255)

 gCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGAt                                                                                                                                    >  1:20341/1‑140 (MQ=255)

       gTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaa                                                                                                                              >  1:445508/1‑140 (MQ=255)

       gTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaa                                                                                                                              >  1:769939/1‑140 (MQ=255)

       gTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaa                                                                                                                              >  1:62586/1‑140 (MQ=255)

        ttAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATaatcaat                                                                                                                             <  1:389067/140‑1 (MQ=255)

          aaGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAatcaatca                                                                                                                           <  2:445508/140‑1 (MQ=255)

             gCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGcc                                                                                                                        <  1:230389/140‑1 (MQ=255)

                  tCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATa                                                                                                                   <  2:20341/140‑1 (MQ=255)

                    aTCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAAt                                                                                                                 <  2:183972/140‑1 (MQ=255)

                                                 ggCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTAcc                                                                                         <  1:223383/135‑1 (MQ=255)

                                                 ggCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTAcc                                                                                         >  2:223383/1‑135 (MQ=255)

                                                        accaGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTc                                                                             <  2:769939/140‑1 (MQ=255)

                                                               tGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCcaca                                                                      <  2:62586/140‑1 (MQ=255)

                                                                gcggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACaa                                                                     >  2:656267/1‑140 (MQ=255)

                                                                gcggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACaa                                                                     >  2:491443/1‑140 (MQ=255)

                                                                 cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTc                                                                             <  1:138453/131‑1 (MQ=255)

                                                                 cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTc                                                                             >  2:138453/1‑131 (MQ=255)

                                                                 cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAAt                                                                    >  1:391244/1‑140 (MQ=255)

                                                                 cggcgCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAAt                                                                    >  1:480152/1‑140 (MQ=255)

                                                                    cgcTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATccc                                                                 <  2:704542/140‑1 (MQ=255)

                                                                      ctctTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGa                                                               <  1:491443/140‑1 (MQ=255)

                                                                                gagaTACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGcc                                                     <  2:460098/140‑1 (MQ=255)

                                                                                   aTACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTg                                                  >  2:142538/1‑140 (MQ=255)

                                                                                      cGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGtt                                                <  1:734400/139‑1 (MQ=255)

                                                                                      cGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGtt                                                >  2:734400/1‑139 (MQ=255)

                                                                                         tGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTAc                                            >  2:423281/1‑140 (MQ=255)

                                                                                           cAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGc                                          <  2:561019/140‑1 (MQ=255)

                                                                                             aaTCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTg                                        >  2:161768/1‑140 (MQ=255)

                                                                                                   ggCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTg                                  >  1:270336/1‑140 (MQ=255)

                                                                                                     ccGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGAt                                <  1:502405/140‑1 (MQ=255)

                                                                                                      cGTTAAACTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATg                               <  1:54928/140‑1 (MQ=255)

                                                                                                          aaaCTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGt                           >  2:436051/1‑140 (MQ=255)

                                                                                                             cTCCAGTTTTTCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTa                        <  1:656267/140‑1 (MQ=255)

                                                                                                                   tttttCTGCCATTACGCGGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGgcgc                  >  1:544599/1‑140 (MQ=255)

                                                                                                                                   cgGACCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGGCGCGGAACGGCGCGCTTtc  <  1:423281/140‑1 (MQ=255)

AGCACGGGTTAAGGCTGGTCATCGGGTCCTGGAAGATCATCGCCACTTCGGCACCCACCAGGTTGCGGCGCTCTTTTTCTGAGATACGCTGCAAATCCTGGCCGTTAAACTCCAGTTTTTCTGCCATTACGCGGCCCGGATAATCAATCAGCCCCATAATCGCCAGTGAACTGACCGACTTACCGGAGCCGGACTCACCCACAATCCCGACCACTTCGCCCTGTTTTACGCTGTAGCTGATGCGGTCTACGGCGCGGAACGGCGCGCTTTC  >  NC_000913/3703533‑3703803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A