Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F60 I1 R1	57	32.8	3585168	30.6%	1097061	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,622	T→G	F382V (TTT→GTT)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,622	0	T	G	96.5%	100.9 / ‑3.5	29	F382V (TTT→GTT)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (16/12); total (17/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.15e-01

ATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAA  >  NC_000913/4279490‑4279750

aTCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGa                                                                                                                           <  1:1700547/140‑1 (MQ=255)

       ttGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTttgtt                                                                                                                    >  2:1408413/1‑140 (MQ=255)

         ggCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGa                                                                                                                  >  2:1249384/1‑140 (MQ=255)

                      tcctGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGt                                                                                                     <  1:1244031/140‑1 (MQ=255)

                             tCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTa                                                                                              <  1:1768130/140‑1 (MQ=255)

                               tGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACg                                                                                            >  1:1536049/1‑140 (MQ=255)

                               tGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACg                                                                                            >  2:1279168/1‑140 (MQ=255)

                                gTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACgg                                                                                           <  1:1388343/140‑1 (MQ=255)

                                  tCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCt                                                                                         >  2:1297963/1‑140 (MQ=255)

                                         tcCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCa                                                                                  >  2:1307035/1‑140 (MQ=255)

                                           cGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATc                                                                                >  1:1571892/1‑140 (MQ=255)

                                              tctctTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTg                                                                             <  2:1536049/140‑1 (MQ=255)

                                                 ctTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTg                                                                          <  1:1279168/140‑1 (MQ=255)

                                                   tACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGAc                                                                        >  2:1283065/1‑140 (MQ=255)

                                                        cGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTa                                                                   >  2:1265234/1‑140 (MQ=255)

                                                          ttCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAAc                                                                 >  1:1350091/1‑140 (MQ=255)

                                                                 gTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTaa                                                          <  1:1615248/140‑1 (MQ=255)

                                                                 gTAGGCAATGGCTCCGGCGCGGTTGTACGTTGGCCTGCTGATGCTGTCCAACATGGAGAAAATCGGCTTTGGTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTaa                                                          <  2:892465/140‑1 (MQ=255)

                                                                    tGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAg                                                       >  2:1293722/1‑140 (MQ=255)

                                                                            cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATg                                               >  2:1739997/1‑140 (MQ=255)

                                                                            cTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATg                                               >  2:1781331/1‑140 (MQ=255)

                                                                                      gATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTcg                                     <  1:1408413/140‑1 (MQ=255)

                                                                                            cGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGActc                               <  1:1283065/140‑1 (MQ=255)

                                                                                                 gCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTg                          >  1:1425975/1‑140 (MQ=255)

                                                                                                   ctgctgATGCTGAGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGAt                        <  1:1297963/140‑1 (MQ=255)

                                                                                                        gatgctgaGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGgtc                   <  1:1265234/140‑1 (MQ=255)

                                                                                                           gctgaGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGGTACGGCCGTTTTCATTGTGCTGACCCGTAACATCGTTACAGGCATCATGTTCGGCTTCCCGGCCCTGGTGATTGgtcgtc                >  2:1307431/1‑140 (MQ=255)

                                                                                                              gaGCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTgg             <  1:1307035/140‑1 (MQ=255)

                                                                                                                gCAACGTGGCGAAAATCGACGTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGtt           >  1:1575698/1‑140 (MQ=255)

                                                                                                                         cGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGaa  >  2:1708896/1‑140 (MQ=255)

ATCACCGTTGGCGTGCTGTTCCTCCTGATTCTGTTCCTCTCTCCGCTCTCTTACCTCGTTCCGGGGTATGCAACGGCTCCGGCGCTGATGTACGTTGGCCTGCTGATGCTGAGCAACGTGGCGAAAATCGACTTTGCTGATTTTGTTGATGCGATGGCGGGTCTGGTTACGGCTGTATTCATCGTGCTGACCTGTAACATCGTAACAGGCATCATGATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAA  >  NC_000913/4279490‑4279750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A