Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A33 F60 I1 R1	23	100.4	3424510	99.4%	3403962	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,281	Δ2 bp	coding (836‑837/1017 nt)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,280	0	G	.	95.3%	344.8 / 12.0	85	coding (838/1017 nt)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand): ref base G (2/2); new base . (38/43); total (40/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.16e-01
*	NC_000913	4,623,281	0	G	.	100.0%	353.8 / NA	81	coding (837/1017 nt)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (38/43); total (38/43)

CTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGC  >  NC_000913/4623143‑4623409

||

ctctTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTgcg                                                                                                                                 <  2:936928/140‑3 (MQ=255)

ctctTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTgcg                                                                                                                                 <  2:23099/140‑3 (MQ=255)

  ctTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGcgcg                                                                                                                               <  2:205471/140‑1 (MQ=255)

  ctTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGcgcg                                                                                                                               <  2:891040/140‑1 (MQ=255)

   tttttCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGcgcgg                                                                                                                              >  1:1382646/1‑139 (MQ=255)

     tttCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGcgcgggt                                                                                                                            >  1:1192702/1‑137 (MQ=255)

        ccTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGat                                                                                                                       >  2:1299383/1‑140 (MQ=255)

         cTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGata                                                                                                                      <  2:1457683/140‑1 (MQ=255)

         cTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGata                                                                                                                      <  1:923364/140‑1 (MQ=255)

         cTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGata                                                                                                                      <  2:1332944/140‑1 (MQ=255)

            ttCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATgg                                                                                                                   >  2:625388/1‑140 (MQ=255)

                 ggAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGTGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCttt                                                                                                              >  2:1355797/1‑140 (MQ=255)

                 ggAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCttt                                                                                                              >  1:1300717/1‑140 (MQ=255)

                 ggAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCttt                                                                                                              >  1:1363527/1‑140 (MQ=255)

                   aaGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCttttt                                                                                                            <  1:1214490/140‑1 (MQ=255)

                      tCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCaa                                                                                                         >  2:209460/1‑140 (MQ=255)

                        aaccaacAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACg                                                                                                       >  2:1521873/1‑140 (MQ=255)

                           caacAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCg                                                                                                    <  2:1404482/140‑1 (MQ=255)

                              cAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGaaa                                                                                                 <  2:370610/140‑1 (MQ=255)

                                gcgcTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATg                                                                                               >  2:1364891/1‑140 (MQ=255)

                                gcgcTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATg                                                                                               <  2:209613/140‑1 (MQ=255)

                                gcgcTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATg                                                                                               >  2:1226621/1‑140 (MQ=255)

                                  gcTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCa                                                                                             >  1:1374203/1‑140 (MQ=255)

                                    ttCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGt                                                                                           >  1:321861/1‑140 (MQ=255)

                                         aCTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAc                                                                                      >  1:362680/1‑140 (MQ=255)

                                          cTCCTGTTGCAGCATATCTGTGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAcg                                                                                     <  1:1355797/140‑1 (MQ=255)

                                          cTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATTGCCTTTTTCAACGTCGAAATGCAGTTCCAcg                                                                                     <  2:1192702/140‑1 (MQ=255)

                                          cTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAcg                                                                                     <  2:495410/140‑1 (MQ=255)

                                          cTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGACTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAcg                                                                                     <  1:393434/140‑1 (MQ=255)

                                           tCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGaa                                                                                                  <  2:56939/126‑1 (MQ=255)

                                           tCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGaa                                                                                                  >  1:56939/1‑126 (MQ=255)

                                           tCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAcgc                                                                                    <  1:166920/140‑1 (MQ=255)

                                              tGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAcgccgc                                                                                 <  1:209460/140‑1 (MQ=255)

                                              tGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAcgccgc                                                                                 <  2:1382646/140‑1 (MQ=255)

                                              tGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCAcgccgc                                                                                 >  1:1079946/1‑140 (MQ=255)

                                                ttGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGccc                                                                               >  1:833915/1‑140 (MQ=255)

                                                  gcagcaTATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCa                                                                             >  2:1560742/1‑140 (MQ=255)

                                                   cagcaTATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAg                                                                            >  2:668450/1‑140 (MQ=255)

                                                      caTATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTa                                                                         <  1:1226621/140‑1 (MQ=255)

                                                      caTATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTa                                                                         <  2:1004361/140‑1 (MQ=255)

                                                       atatCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTaa                                                                        <  2:872492/140‑1 (MQ=255)

                                                       atatCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTaa                                                                        >  2:1258390/1‑140 (MQ=255)

                                                        tatCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTaaa                                                                       >  1:958980/1‑140 (MQ=255)

                                                          tCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGc                                                                     >  1:1098795/1‑140 (MQ=255)

                                                          tCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGc                                                                     >  2:233297/1‑140 (MQ=255)

                                                            tGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGt                                                                   <  2:321861/140‑1 (MQ=255)

                                                             gcgcGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGtt                                                                  >  2:492065/1‑140 (MQ=255)

                                                              cgcgGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTc                                                                 >  1:781142/1‑140 (MQ=255)

                                                              cgcgGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTc                                                                 >  1:1114269/1‑140 (MQ=255)

                                                                cgGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCAt                                                               >  1:57348/1‑140 (MQ=255)

                                                                   tACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCca                                                            >  1:430297/1‑140 (MQ=255)

                                                                   caCAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCca                                                            >  1:119342/2‑140 (MQ=255)

                                                                    aCAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCcag                                                           <  1:1560742/140‑1 (MQ=255)

                                                                    aCAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCcag                                                           <  1:1364891/140‑1 (MQ=255)

                                                                    aCAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCcag                                                           <  2:1114269/140‑1 (MQ=255)

                                                                    aCAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCcag                                                           <  1:49510/140‑1 (MQ=255)

                                                                     cAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCcagc                                                          >  2:1094235/1‑140 (MQ=255)

                                                                        gCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGa                                                       <  2:1180480/140‑1 (MQ=255)

                                                                        gCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGa                                                       <  1:568968/140‑1 (MQ=255)

                                                                         cAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGAt                                                      <  2:57348/140‑1 (MQ=255)

                                                                             cTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCga                                                  >  2:1572273/1‑140 (MQ=255)

                                                                             cTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCga                                                  >  2:431551/1‑140 (MQ=255)

                                                                             cTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCga                                                  >  2:983720/1‑140 (MQ=255)

                                                                             cTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCga                                                  >  2:1189236/1‑140 (MQ=255)

                                                                             cTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCga                                                  >  1:66507/1‑140 (MQ=255)

                                                                              ttGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCgag                                                 <  2:210399/140‑1 (MQ=255)

                                                                               tGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCgaga                                                <  2:1300717/140‑1 (MQ=255)

                                                                               tGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCgaga                                                <  2:781142/140‑1 (MQ=255)

                                                                                   gtcgtcCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGc                                            <  1:668450/140‑1 (MQ=255)

                                                                                      gtcCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCgg                                         >  1:596530/1‑140 (MQ=255)

                                                                                           gCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCt                                    <  2:1363527/140‑1 (MQ=255)

                                                                                              aGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGAc                                 <  2:833915/140‑1 (MQ=255)

                                                                                              aGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGAc                                 <  1:1241371/140‑1 (MQ=255)

                                                                                                ggCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCg                               <  2:958980/140‑1 (MQ=255)

                                                                                                ggCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCg                               <  1:431551/140‑1 (MQ=255)

                                                                                                 gCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAGGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGa                              <  2:362680/140‑1 (MQ=255)

                                                                                                  cTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGaa                             >  1:19238/1‑140 (MQ=255)

                                                                                                  cTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGaa                             >  1:995189/1‑140 (MQ=255)

                                                                                                      cAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTc                         <  1:1572273/140‑1 (MQ=255)

                                                                                                          ggcgCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCAtt                     <  1:625388/140‑1 (MQ=255)

                                                                                                          ggcgCGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGATAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCAtt                     <  1:1521873/140‑1 (MQ=255)

                                                                                                           gcgcGGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTc                    >  1:171043/1‑140 (MQ=255)

                                                                                                            cgcgGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTcc                   <  1:1299383/140‑1 (MQ=255)

                                                                                                             gcgGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTccc                  <  1:1189236/140‑1 (MQ=255)

                                                                                                             gcgGGGTTGAGGCTGTCGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTccc                  <  1:492065/140‑1 (MQ=255)

                                                                                                                             cGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGc  >  2:1015870/1‑140 (MQ=255)

                                                                                                                             cGGTAAACACCT‑‑GCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGc  <  2:1374203/140‑1 (MQ=255)

||

CTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGC  >  NC_000913/4623143‑4623409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A