Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F60 I1 R1	13	66.1	2245786	99.1%	2225573	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,769,734	A→G	intergenic (+54/+509)	yibV → / ← yibH	PF15596 family protein YibV/inner membrane protein YibH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,769,734	0	A	G	100.0%	150.4 / NA	39	intergenic (+54/+509)	yibV/yibH	PF15596 family protein YibV/inner membrane protein YibH
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/28); total (11/28)

AAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTA  >  NC_000913/3769598‑3769867

aaaaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTtagtag                                                                                                                                    >  2:942463/1‑140 (MQ=255)

 aaaTCTAAATCCACTCCCACTTACCGCTGATGGCATTGACACAGTAAACAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTagtagt                                                                                                                                   <  2:70197/140‑1 (MQ=255)

 aaaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTagtagt                                                                                                                                   <  2:1039274/140‑1 (MQ=255)

 aaaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTagtagt                                                                                                                                   <  1:617685/140‑1 (MQ=255)

  aaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAgtagta                                                                                                                                  <  2:26956/140‑1 (MQ=255)

  aaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAgtagta                                                                                                                                  <  1:330803/140‑1 (MQ=255)

  aaTCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAgtagta                                                                                                                                  <  1:630709/140‑1 (MQ=255)

          tCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATg                                                                                                                          <  2:406038/140‑1 (MQ=255)

           ccACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATgg                                                                                                                         <  1:806768/140‑1 (MQ=255)

                  aaCTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATtga                                                                                                                  <  1:427476/140‑1 (MQ=255)

                    cTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATtgatg                                                                                                                <  2:489098/140‑1 (MQ=255)

                    cTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATtgatg                                                                                                                <  1:477562/140‑1 (MQ=255)

                            tGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCtata                                                                                                        <  2:233697/140‑1 (MQ=255)

                            tGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCtata                                                                                                        <  1:354991/140‑1 (MQ=255)

                                   aTTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCa                                                                                                 <  2:824459/140‑1 (MQ=255)

                                    ttGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCAt                                                                                                <  2:297835/140‑1 (MQ=255)

                                    ttGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCAt                                                                                                <  2:718773/140‑1 (MQ=255)

                                    ttGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCAt                                                                                                <  2:692974/140‑1 (MQ=255)

                                       aaaCAGTAAATAAAATTAAAGATAAGAATAATAGAGATCCTTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGa                                                                                             <  2:107227/140‑1 (MQ=255)

                                            gTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGatgaat                                                                                        >  2:746942/1‑140 (MQ=255)

                                              aaataaaataAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGatgaatga                                                                                      <  1:984561/140‑1 (MQ=255)

                                                     ataaaaGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTa                                                                               >  2:776570/1‑140 (MQ=255)

                                                        aaaGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAAtt                                                                            <  2:385931/140‑1 (MQ=255)

                                                         aaGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAAttt                                                                           <  1:411849/140‑1 (MQ=255)

                                                          aGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAAtttt                                                                          <  2:413922/140‑1 (MQ=255)

                                                            aTAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATtttat                                                                        <  2:409694/140‑1 (MQ=255)

                                                                      aaaaaaTCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAatat                                                              <  1:308947/140‑1 (MQ=255)

                                                                           aTCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATtaa                                                         <  2:775253/140‑1 (MQ=255)

                                                                           aTCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATtaa                                                         <  1:540993/140‑1 (MQ=255)

                                                                               ttGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAac                                                     >  2:937988/1‑140 (MQ=255)

                                                                                tGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAact                                                    <  1:9110/140‑1 (MQ=255)

                                                                                  atatAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAactca                                                  <  1:855013/140‑1 (MQ=255)

                                                                                            aGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGa                                        >  1:396817/1‑140 (MQ=255)

                                                                                            aGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGa                                        >  2:46439/1‑140 (MQ=255)

                                                                                             gTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAg                                       >  1:1102765/1‑140 (MQ=255)

                                                                                                        tAGTGATGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACt                            >  1:848646/1‑140 (MQ=255)

                                                                                                        tAGTGACGATAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACt                            <  2:884992/140‑1 (MQ=255)

                                                                                                             atgatAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGtt                       >  2:253481/1‑140 (MQ=255)

                                                                                                              tgatAAAAATATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGttt                      >  2:259982/1‑140 (MQ=255)

                                                                                                                  aaaaaTATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTtata                  <  1:937988/140‑1 (MQ=255)

                                                                                                                  aaaaaTATAAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTtata                  <  1:746942/140‑1 (MQ=255)

                                                                                                                       tataAAATTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGaa             <  1:46439/140‑1 (MQ=255)

                                                                                                                          aaaaTTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATAc          >  2:166242/1‑140 (MQ=255)

                                                                                                                            aaTTGAGAGTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGa        <  1:253481/140‑1 (MQ=255)

                                                                                                                                  gagTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTa  <  2:819202/140‑1 (MQ=255)

                                                                                                                                  gagTTAGTAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTa  >  1:819202/1‑140 (MQ=255)

AAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTA  >  NC_000913/3769598‑3769867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A