Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A41 F60 I1 R1 13 41.9 1423038 99.2% 1411653 138.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,354,4870CT100.0% 131.2 / NA 37intergenic (‑437/‑238)yhcC/gltBradical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/18);  total (19/18)

GTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC  >  NC_000913/3354351‑3354620
                                                                                                                                         |                                                                                                                                     
gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGc                                                                                                                                    >  2:667215/1‑140 (MQ=255)
gTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGc                                                                                                                                    >  1:686911/1‑140 (MQ=255)
        ttGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGa                                                                                                                            <  2:520653/140‑1 (MQ=255)
           aCTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATaa                                                                                                                         >  2:342642/1‑140 (MQ=255)
               tttCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGa                                                                                                                            >  1:275457/1‑133 (MQ=255)
               tttCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGa                                                                                                                            <  2:275457/133‑1 (MQ=255)
                         gACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCt                                                                                                           >  1:692627/1‑140 (MQ=255)
                            ttCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGa                                                                                                        >  2:224173/1‑140 (MQ=255)
                             tCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGaa                                                                                                       >  1:213732/1‑140 (MQ=255)
                              cccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAg                                                                                                      >  2:268111/1‑140 (MQ=255)
                              cccTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAg                                                                                                      <  2:705223/140‑1 (MQ=255)
                                 ttttAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCtt                                                                                                   >  1:67248/1‑140 (MQ=255)
                                     aGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTg                                                                                               >  1:606205/1‑140 (MQ=255)
                                     aGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTg                                                                                               <  2:686911/140‑1 (MQ=255)
                                         ttAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATg                                                                                           >  1:573510/1‑140 (MQ=255)
                                          tAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGa                                                                                          >  2:3580/1‑140 (MQ=255)
                                          tAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGa                                                                                          <  2:606205/140‑1 (MQ=255)
                                              gATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGagcag                                                                                      <  1:268111/140‑1 (MQ=255)
                                                 aaaaTCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCt                                                                                   <  2:67248/140‑1 (MQ=255)
                                                       cATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCtcat                                                                             <  1:667215/140‑1 (MQ=255)
                                                         ttttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTAGAAGCTTTCTGGATGAGCAGCCTGCtcatca                                                                           <  1:224173/140‑1 (MQ=255)
                                                          tttAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTcatcat                                                                          >  2:268990/1‑140 (MQ=255)
                                                                 tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                   <  1:34200/140‑1 (MQ=255)
                                                                 tCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                   <  1:558600/140‑1 (MQ=255)
                                                                   aGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCa                                                                 <  2:83942/140‑1 (MQ=255)
                                                                    gTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                   <  2:643397/137‑1 (MQ=255)
                                                                    gTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATg                                                                   >  1:643397/1‑137 (MQ=255)
                                                                    gTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAg                                                                <  2:254938/140‑1 (MQ=255)
                                                                    atCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCATTTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCa                                                                 <  2:250442/139‑1 (MQ=255)
                                                                       aTTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTaa                                                             <  1:342642/140‑1 (MQ=255)
                                                                            ataaAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTgag                                                        <  1:41179/140‑1 (MQ=255)
                                                                                                      ttCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGAt                              >  2:204202/1‑140 (MQ=255)
                                                                                                          gTACCAATAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGa                          >  1:337143/1‑140 (MQ=255)
                                                                                                                aTAAGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGa                    >  1:62975/1‑140 (MQ=255)
                                                                                                                  aaGCTTGCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGaca                  >  1:210539/1‑140 (MQ=255)
                                                                                                                        gCCA‑TTTGACCTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAgg            >  1:549521/1‑140 (MQ=255)
                                                                                                                                   cTGTATTAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGc  <  1:3580/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GTCAGGAATTGACTGTTTCTCTAACGACTTCCCTTTTAGCCTTAAAGATAAAATCCATTTTAATTTCAGTCATTTAATAAAGAATTTTGCGCTAAAGCACATTTCTGTACCAATAAGCTTGCCA‑TTTGACCTGTATCAGCTTTCCCGATAAGTTGGAAATCCGCTGGAAGCTTTCTGGATGAGCAGCCTGCTCATCATATTTATGCAGTAATTGAGATCCCCTCTTCACCGTATTAACCGATGCGAAAAGGACAACAAGGGGGCGAATGC  >  NC_000913/3354351‑3354620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A