Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F60 I1 R1	20	72.4	2489094	99.1%	2466692	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,412,278	T→C	L209P (CTC→CCC)	yhdJ →	DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,412,278	0	T	C	98.3%	211.3 / NA	59	L209P (CTC→CCC)	yhdJ	DNA adenine methyltransferase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (37/21); total (38/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAAAATCCTCCACAGCCTTACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACAGCGAGTACATCAAAATGG  >  NC_000913/3412141‑3412406

aaaaaaCCCTCCACAGCCTTACAATCATAAAAAAGCCCGGGGTAACGTTGGGGTTTTTCCGCGCGTGCGTTTTTTGATGGATAAATATAAAACCCCCCCGACGAAAAACCCGGAAGCCTTACTGAAACGCATTATTCCCg                                                                                                                                <  2:421464/139‑1 (MQ=255)

 aaaaaTCCTCCACAGCCTTACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATAAATATAAAACCCCCCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGc                                                                                                                               <  1:1081330/140‑1 (MQ=255)

  aaaaTCCTCCACAGCCTTACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGcc                                                                                                                              >  2:71768/1‑140 (MQ=255)

    aaTCCTCCACAGCCTTACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCctc                                                                                                                            >  2:1113538/1‑140 (MQ=255)

       cctccACACCCTTACAATCAAAAAAAAGTCCGGGTTAACGTTTGGGTTTTCCCGCGCGTGCGTTTTTTGATGGATAAATATAAAACCCCCCCGACGCAAAACCCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTc                                                                                                                         <  2:827471/140‑1 (MQ=255)

             caGCCTTACAATCATGAAAAAGGACTGGGTAACGTTTAGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCCCCCGACGCAAAACCCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAAccc                                                                                                                   <  1:529083/140‑1 (MQ=255)

             caGCCTTACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAAccc                                                                                                                   >  2:299718/1‑140 (MQ=255)

                 cTTACAAGCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGc                                                                                                               >  2:781495/1‑140 (MQ=255)

                   tACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGa                                                                                                             >  1:35958/1‑140 (MQ=255)

                   tACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGa                                                                                                             >  1:998617/1‑140 (MQ=255)

                   tACAATCATCAAAAACTCCCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGa                                                                                                             >  2:772048/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGtt                                                                                                      >  1:1176802/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTGTTCCAACCCAGGCGATATCGtt                                                                                                      >  1:489670/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGtt                                                                                                      >  2:580563/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGtt                                                                                                      >  1:690171/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGtt                                                                                                      >  1:811095/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGtt                                                                                                      >  2:710248/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGtt                                                                                                      >  1:1050217/1‑140 (MQ=255)

                          atcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACACATTATTACCGTCTATTCCAACCCAGGCGATATCGtt                                                                                                      >  1:505025/1‑140 (MQ=255)

                           tcaAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTtc                                                                                                     >  2:387906/1‑140 (MQ=255)

                                 aGTCCCGGATACCGTTTGGATTTTCCCGCGGGTGCTTTTTTTGATGGATGAAAATAAAACCCCCCCGACGCAAAAGCGGGAAGCCTTACTGAAACACATTATCCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGAcc                                                                                               <  2:998617/140‑1 (MQ=255)

                                    ccggggTAACGTTTGGGATTTTGCGCGCGTGCGTTATATGATGGATAAATATAAAACCCCCCCGACGCAAAACCCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGt                                                                                            <  1:489326/137‑1 (MQ=255)

                                    aCCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCTGCCTCTTTCAATCCTGGCGATGTCGTTCTCGACCCGt                                                                                            >  1:571586/1‑140 (MQ=255)

                                         gTAACTTTGGGGTTTTCCCGCGCGTGCTTTTCTTGCTGGTTGAATATAAAACCCCCCCGACGAAAAACCCGGAAGCTTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCt                                                                                       <  2:505025/140‑1 (MQ=255)

                                         gTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATAATTCCCGCGTCTTCCAACCCAGGCGCTATCGTTCTCGACCCGTTTGCt                                                                                       >  2:489326/1‑140 (MQ=255)

                                            aCGTTTGGGTTTTCCCGCGCGTGCTTTTTTTGATGGATAAATATAAAACCCCCCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGt                                                                                    <  2:811095/140‑1 (MQ=255)

                                                  gggATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCttt                                                                              >  1:1147615/1‑140 (MQ=255)

                                                  gggATTTTCCGCGCGTGCGTTATTTGATGGATGAATATAAAACCCCCCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCttt                                                                              <  1:155655/140‑1 (MQ=255)

                                                       ttcccGCGCGTGCGTTTTTTGAGGGATAAATATAAAACCCCCCCGACGCAAAACCCGGAAGCCTTACTAAACCGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTtactac                                                                         <  2:749917/137‑1 (MQ=255)

                                                        ttCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCAAACCCAGGCGATTTGGAACTCGACCGGTTTACTGGTAGCTTTACTAcc                                                                        >  2:596770/1‑140 (MQ=255)

                                                         tCCGCGCGTGCGTTTTTTGAGGTATAAATATGAAATCCCCCCGACGCATAAACCGGAAGCCTTACTGAACCGCTTTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCg                                                                       <  2:489670/140‑1 (MQ=255)

                                                               ggtgcttTGTTTGATGGATAAATATAAAACCCCCCCGACGCAAAAGCCGGAAGCCTTACTAAAACCCCTTATTCCCGCCTCTGCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCg                                                                 <  2:737504/139‑1 (MQ=255)

                                                               cgtgcgtTATTTGATGGATGAATATGGAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCg                                                                 >  1:883368/1‑140 (MQ=255)

                                                                   cgtTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGc                                                             >  1:303501/1‑140 (MQ=255)

                                                                                      atGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGaaac                                          >  2:1161839/1‑139 (MQ=255)

                                                                                      aggAAACCCCCCCGACGGAAAAGCCGGAAGCCTTATTAACCGGCATTCTCCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGaaaa                                          <  2:445640/138‑1 (MQ=255)

                                                                                         aaaaCCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTAGTACCGGTGCCGTAGACATCGCGAGCGGACGAAAATTc                                       >  1:407221/1‑140 (MQ=255)

                                                                                         aaaaCCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTAGTACCGGTCCCGCAGACATCGCCAGCGGACGAAAATTc                                       >  1:445640/1‑140 (MQ=255)

                                                                                           acccccccGCCGCAAAACCCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCAt                                     <  2:74329/135‑1 (MQ=255)

                                                                                           acccccccGACGCAAAAACCGAAACGCTTCCTGAAACTCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCAt                                     <  1:550546/135‑1 (MQ=255)

                                                                                           acccccccGACGAAACACCCGGCAGCCTTTCGGAACCACATTTTTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCAt                                     <  1:387906/135‑1 (MQ=255)

                                                                                           acccccccGACGAAAAAACCGGAAGCCTTACTAAACCGCATTTTGCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCAt                                     <  2:1167621/135‑1 (MQ=255)

                                                                                             ccaccCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACGGGTGCCGGAGCGATGGCCAGCGGACGAAAATTCATTg                                   >  1:321819/1‑140 (MQ=255)

                                                                                               accCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGt                                 >  2:964330/1‑140 (MQ=255)

                                                                                                cccGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTa                                >  1:787527/1‑140 (MQ=255)

                                                                                                cccGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTa                                >  2:752329/1‑140 (MQ=255)

                                                                                                cccGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTa                                >  2:1078292/1‑140 (MQ=255)

                                                                                                cccGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTa                                >  2:166197/1‑140 (MQ=255)

                                                                                                cccGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTa                                >  2:195567/1‑140 (MQ=255)

                                                                                                cccGACGAAAAACCCGGAAGCTTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTa                                <  2:690171/140‑1 (MQ=255)

                                                                                                cccGACGAAAAACCCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTa                                <  1:1041496/140‑1 (MQ=255)

                                                                                                 ccGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTAt                               >  1:60725/1‑140 (MQ=255)

                                                                                                  cgacgaAAAACCCGGAACCCTTGCTGAAACGTATTTTTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTAtt                              <  2:321819/140‑1 (MQ=255)

                                                                                                     cGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTgag                           >  2:1144270/1‑140 (MQ=255)

                                                                                                      gCAAAACCCGGAAGCGTTACTATAATGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTgaga                          <  1:580563/140‑1 (MQ=255)

                                                                                                       aaaaacCCGGAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGAt                         <  2:35958/139‑1 (MQ=255)

                                                                                                            cccGGACGCCTTACCGAACCAATTTATCCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACa                    <  2:1050217/139‑1 (MQ=255)

                                                                                                                gAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACAGCGa                >  1:890198/1‑140 (MQ=255)

                                                                                                                gAAGCCTTACTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACAGCGa                >  1:827246/1‑140 (MQ=255)

                                                                                                                        aCTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGTAAATTCAGTGGAATAGAGATCTACAGCGAGTACATCa        >  1:319846/1‑140 (MQ=255)

                                                                                                                        aCTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACAGCGAg               <  1:952889/133‑1 (MQ=255)

                                                                                                                        aCTGAAACGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACAGCGAg               >  2:952889/1‑133 (MQ=255)

                                                                                                                              aCGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACAGCGAGTACATCAAAATgg  >  2:30108/1‑140 (MQ=255)

                                                                                                                              aCGCATTATTCCCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCAGACGAAAAGTCAATGGTATTGAGATAAACAACGAGTACATCAAAATgg  >  2:366147/1‑140 (MQ=255)

CAAAAATCCTCCACAGCCTTACAATCATCAAAAAGTACCGGGTAACGTTTGGGATTTTCCGCGCGTGCGTTATTTGATGGATGAATATGAAAACCACCCGACGCAAAAACCGGAAGCCTTACTGAAACGCATTATTCTCGCCTCTTCCAACCCAGGCGATATCGTTCTCGACCCGTTTGCTGGTAGCTTTACTACCGGTGCCGTAGCCATCGCCAGCGGACGAAAATTCATTGGTATTGAGATCAACAGCGAGTACATCAAAATGG  >  NC_000913/3412141‑3412406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A