Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F60 I1 R1 20 86.3 2971396 99.1% 2944653 139.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,349,798 G→C S4R (AGC→AGG)  mtgA ← peptidoglycan glycosyltransferase MtgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,349,7980GC98.8% 304.5 / ‑3.7 82S4R (AGC→AGG) mtgApeptidoglycan glycosyltransferase MtgA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (39/42);  total (39/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCAT  >  NC_000913/3349666‑3349933
                                                                                                                                    |                                                                                                                                       
tCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTGAGCGGCTGTTAc                                                                                                                                  >  2:1357342/1‑140 (MQ=255)
tCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTAc                                                                                                                                  >  2:292621/1‑140 (MQ=255)
tCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTAc                                                                                                                                  >  2:170401/1‑140 (MQ=255)
   cTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCCAACGAAACTAAAAACCGTTAAGCGCCTTTTACTCa                                                                                                                               >  2:558771/1‑140 (MQ=255)
     cGACCATTACCGCT‑‑GAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAAAAAACGGCAAACGAAACTAAACACCGTTAAGCGCCTTTTTCTCATTCa                                                                                                                           >  2:687393/1‑140 (MQ=255)
         cATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGc                                                                                                                         <  1:159891/140‑1 (MQ=255)
         cATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGc                                                                                                                         <  2:162661/140‑1 (MQ=255)
          aTTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGcc                                                                                                                        >  1:740681/1‑140 (MQ=255)
                 cTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCa                                                                                                                 >  2:147418/1‑140 (MQ=255)
                  tGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAg                                                                                                                <  1:1301425/140‑1 (MQ=255)
                    ataaGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCa                                                                                                              >  1:1423300/3‑140 (MQ=255)
                    ataaGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCa                                                                                                              <  2:1423300/138‑1 (MQ=255)
                     gaAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGTACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCAc                                                                                                             >  2:1164056/1‑140 (MQ=255)
                     gaAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCAc                                                                                                             >  2:766039/1‑140 (MQ=255)
                       aGGAAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCAcgc                                                                                                           <  2:1392249/140‑1 (MQ=255)
                        gggAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCAcgcg                                                                                                          >  2:98909/1‑140 (MQ=255)
                             cAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGaaa                                                                                                     <  1:1216685/140‑1 (MQ=255)
                              aGGGGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAAc                                                                                                    <  1:1049524/140‑1 (MQ=255)
                              aGGCGCAACGCTAAACAACGCGACGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAAc                                                                                                    <  1:98909/140‑1 (MQ=255)
                                gcgcAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCa                                                                                                  >  2:283133/1‑140 (MQ=255)
                                gcgcAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCATTTACTCGTTCAGCCAGAACCGGCACGCGGGAAACCa                                                                                                  >  1:540012/1‑140 (MQ=255)
                                 cgcAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGTACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAg                                                                                                 <  1:1164056/140‑1 (MQ=255)
                                 cgcAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAg                                                                                                 <  1:766039/140‑1 (MQ=255)
                                 cgcAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAg                                                                                                 <  1:292621/140‑1 (MQ=255)
                                   cAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAg                                                                                                 >  2:238267/1‑138 (MQ=255)
                                   cAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAg                                                                                                 <  1:238267/138‑1 (MQ=255)
                                   cAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCt                                                                                               >  2:1454489/1‑140 (MQ=255)
                                   cAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCt                                                                                               >  1:1289136/1‑140 (MQ=255)
                                   aaaCGCTAAACAACGGCTGGCCGCCGCCCCAGACAACGGTGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCt                                                                                               <  2:670685/139‑1 (MQ=255)
                                     aCGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTa                                                                                             >  2:49280/1‑140 (MQ=255)
                                      cGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTAt                                                                                            <  1:147418/140‑1 (MQ=255)
                                                cgcgATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCt                                                                                  >  2:1026288/1‑140 (MQ=255)
                                                cgcgATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCt                                                                                  <  1:1026288/140‑1 (MQ=255)
                                                cgcgATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAACCCGAACCAGAACGCGGTAAACCAGCTTATCAATGCCGCt                                                                                  >  1:675160/1‑140 (MQ=255)
                                                cgcgATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTAATCATTCAGGCAGAACCGGCACGCGGGAAACCAGCTTATCAATGCCGCt                                                                                  >  2:550904/1‑140 (MQ=255)
                                                 gcgATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTc                                                                                 >  2:113930/1‑140 (MQ=255)
                                                 gcgATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTc                                                                                 >  2:36272/1‑140 (MQ=255)
                                                    aTGCCCCCGCCCCAGAACACGGTGAGGACAACCATTAACGGCAAAAGATAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTcgcc                                                                              <  2:497165/140‑1 (MQ=255)
                                                     tGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTcgccg                                                                             >  2:1135516/1‑140 (MQ=255)
                                                     tGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTcgccg                                                                             <  1:1065803/140‑1 (MQ=255)
                                                      gCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTcgccgc                                                                            <  1:746740/140‑1 (MQ=255)
                                                       cccccGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCt                                                                           >  2:204114/1‑140 (MQ=255)
                                                           cGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTcgc                                                                               >  2:18649/1‑132 (MQ=255)
                                                           cGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTcgc                                                                               <  1:18649/132‑1 (MQ=255)
                                                             ccccAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCa                                                                     <  1:1226298/140‑1 (MQ=255)
                                                                       cGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTgcg                                                           <  1:953199/140‑1 (MQ=255)
                                                                       cGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTgcg                                                           <  1:1362355/140‑1 (MQ=255)
                                                                       cGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTgcg                                                           <  1:170401/140‑1 (MQ=255)
                                                                       cGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTgcg                                                           <  2:794539/140‑1 (MQ=255)
                                                                        ggCGAGGACAACCATTAACCGCTAACGAATACGGCGAGCGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTgcgc                                                          <  1:413826/140‑1 (MQ=255)
                                                                        ggCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTgcgc                                                          >  1:468922/1‑140 (MQ=255)
                                                                         gCGAGGACATCCATTCACCGCATAAGAAAGCGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGcc                                                         <  1:687393/140‑1 (MQ=255)
                                                                         gCGAGGACAACCATTGACCGCAAAACAAACCGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGcc                                                         <  1:550904/140‑1 (MQ=255)
                                                                         gCGAGGACAACCATTAACCGCACAAGAAAACAGCGAACGTAACTAAACTCCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGcc                                                         <  2:594851/140‑1 (MQ=255)
                                                                          cGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCa                                                        <  1:36272/140‑1 (MQ=255)
                                                                          cGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCa                                                        <  2:138179/140‑1 (MQ=255)
                                                                          cGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCa                                                        <  2:1333534/140‑1 (MQ=255)
                                                                           gAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAg                                                       <  1:160013/140‑1 (MQ=255)
                                                                              gACAGCCATTAACCGCAAAAGAAAGCGGCGAACGAAACTAAACACCGTTAAGCGCCCTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCat                                                    <  2:758909/140‑1 (MQ=255)
                                                                              gACAACCATTAACCGTAAAAGAAAATGGCCACCGAAATTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCat                                                    <  1:669854/140‑1 (MQ=255)
                                                                              gACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCat                                                    <  1:1198019/140‑1 (MQ=255)
                                                                              gACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTAGCCGCTTCTGCAATGTTCTGCGCCAGCat                                                    <  2:1289136/140‑1 (MQ=255)
                                                                                 aaCCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCGGCTTCTCCAATGTTCTGCGCCAGCatata                                                 >  1:594851/1‑140 (MQ=255)
                                                                                  aCCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCatata                                                 >  1:281526/1‑139 (MQ=255)
                                                                                  aCCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCatata                                                 <  2:281526/139‑1 (MQ=255)
                                                                                    cATTAACCGCAAAAGAAAACGGCGAACGAACCTCATCACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGc                                              <  2:712241/140‑1 (MQ=255)
                                                                                        aaCCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGACGCTTCTACAAAGTCCTGCGCCAGCATATATGCTggg                                          >  1:728217/1‑140 (MQ=255)
                                                                                             cAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGggtggt                                     <  1:1091777/140‑1 (MQ=255)
                                                                                             cAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTCCTGCAATATTCGGCGCCAGCAGATATGCTGGggtggt                                     >  1:538434/1‑140 (MQ=255)
                                                                                             cAAAAGAAAACGGCGAACGAAACTAAACACCGGTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGggtggt                                     >  2:1016636/1‑140 (MQ=255)
                                                                                                        ggCGAACGAAACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTAt                          >  1:1126158/1‑140 (MQ=255)
                                                                                                             acgaaACTAAACACCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCt                     >  1:759083/1‑140 (MQ=255)
                                                                                                                  aCTAAACACCGTTAAGGCCCTTGTACTCATTCATCCAGATCCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTtcatc                <  2:675160/140‑1 (MQ=255)
                                                                                                                         acCGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACg         <  1:1454489/140‑1 (MQ=255)
                                                                                                                           cGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGat       >  1:781154/1‑140 (MQ=255)
                                                                                                                           cGTTAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTAACAGTCGTATTGTATTCATCAACCACGat       >  1:683921/1‑140 (MQ=255)
                                                                                                                             ttAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGAGAATCTTGTTGTCTACATCAACCACGatat     >  1:456871/1‑140 (MQ=255)
                                                                                                                             ttAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGatat     >  1:304457/1‑140 (MQ=255)
                                                                                                                              tAAGCGCCTTTTACTCATTCAGCCATAACAAGCACGCCGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATatc    <  1:558771/140‑1 (MQ=255)
                                                                                                                              tAAGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATatc    <  2:304457/140‑1 (MQ=255)
                                                                                                                                aGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATatcat  >  2:1716/1‑140 (MQ=255)
                                                                                                                                aGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATatcat  >  1:1026691/1‑140 (MQ=255)
                                                                                                                                aGCGCCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATAGATGCTGGGATGGGGACTATCTTATTGTCTTCATCAACCACGATatcat  >  2:503165/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TCGCTCGACCATTACCGCTGAGAAGGGAACAGGCGCAACGCTAAACAACGCGATGCCCCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCAT  >  NC_000913/3349666‑3349933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A