Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F60 I1 R1	18	148.4	5087586	98.9%	5031622	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,429,788	T→C	*185R (TGA→CGA) Q79R (CAA→CGA)	yrdA → yrdB ←	protein YrdA conserved protein YrdB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,429,788	0	T	C	98.7%	576.2 / NA	156	*185R (TGA→CGA) Q79R (CAA→CGA)	yrdA yrdB	protein YrdA conserved protein YrdB
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (79/74); minor base A (0/2); total (79/77)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

ATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTTGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCAGAGATCGC  >  NC_000913/3429652‑3429924

atatCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGat                                                                                                                                       <  2:2226151/140‑1 (MQ=255)

   tCTGTATCTCGGTAGCCTCGTCAAACAGATCCGCCAGTTAAGTGATGATGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTTGatcat                                                                                                                                    <  2:876745/140‑1 (MQ=255)

         tctcGGTAGCCCCGTCAAACAGGTCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCAc                                                                                                                              >  1:1959243/1‑140 (MQ=255)

          ctcGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACt                                                                                                                             <  1:1688922/140‑1 (MQ=255)

            cGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACTAATATCTGGATCAGGGGAACCAGACGCAGCGTCGATCATCTTCACttt                                                                                                                           >  2:697079/1‑140 (MQ=255)

            cGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCATACGCAGCCTCGATCATCTTCACttt                                                                                                                           >  1:999722/1‑140 (MQ=255)

             ggTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTg                                                                                                                          >  1:2533438/1‑140 (MQ=255)

              gTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACt                                                                                                                             >  2:2409041/1‑136 (MQ=255)

               tAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGtt                                                                                                                        <  1:1400042/140‑1 (MQ=255)

               tAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAAAATCTGGATCAGGGTCAGCAGACCCAGCCTCCATCATCTTAACTTTGtt                                                                                                                        >  2:1164756/1‑140 (MQ=255)

                aGCCCCGTCAAACAGATGCGCCTGTTAAGTGCTGACGAGCAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTc                                                                                                                       <  2:917555/140‑1 (MQ=255)

                 gCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTcc                                                                                                                      >  2:1966140/1‑140 (MQ=255)

                 gCCCCGTCAAACAGATCCGCCCATTCAGTAATGAAGAAAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTcc                                                                                                                      <  1:1199084/140‑1 (MQ=255)

                  ccccGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCt                                                                                                                     <  2:1250078/140‑1 (MQ=255)

                     cGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGaa                                                                                                                  <  2:1813046/140‑1 (MQ=255)

                     cGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGaa                                                                                                                  <  1:1787289/140‑1 (MQ=255)

                       tCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAAtt                                                                                                                >  2:387275/1‑140 (MQ=255)

                       tCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAAtt                                                                                                                >  1:117382/1‑140 (MQ=255)

                       tCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGAACAGGGTAAACAGTCCCAGCCTCGATGATCTACACTTTGTTCCTGAAtt                                                                                                                >  2:712714/1‑140 (MQ=255)

                                 ccGGCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATAACTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCg                                                                                                      <  2:1412732/140‑1 (MQ=255)

                                 ccGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCg                                                                                                      >  1:48303/1‑140 (MQ=255)

                                 ccGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCACGATCATGTTCACTATGTTCCCGAATTAAGTTTTCCg                                                                                                      >  1:1020519/1‑140 (MQ=255)

                                 ccGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACAAGACCCAGACTCTATCATCTTCACTATGTTACTGAATTAAGTTTTCCg                                                                                                      >  2:879519/1‑140 (MQ=255)

                                   gCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGct                                                                                                    >  2:1686003/1‑140 (MQ=255)

                                    cccGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTTCTCCTAAATTGAGttttaggcgt                                                                                                   >  2:2340697/1‑134 (MQ=255)

                                    cccGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGctt                                                                                                   <  2:103787/140‑1 (MQ=255)

                                    cccGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGctt                                                                                                   >  1:1285614/1‑140 (MQ=255)

                                    cccGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTGTGTTCCTGACTTAAGTTTTCCGctt                                                                                                   >  1:860799/1‑140 (MQ=255)

                                     ccGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACCTTGTTCCTGAATTAAGTTTTCCGcttc                                                                                                  >  1:1717268/1‑140 (MQ=255)

                                      cGTTAAGTGATGAAGGGAAGGCCGGTTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGcttct                                                                                                 <  1:834155/140‑1 (MQ=255)

                                      cGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGcttct                                                                                                 <  2:1663601/140‑1 (MQ=255)

                                         tAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATTTTCACTTGGTTACTTAATTAAATTTTCCGcttcttct                                                                                              >  1:1008649/1‑140 (MQ=255)

                                           aGTGATGAAGAGAAGTCTGGGTCACTCTAATCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCttcttcttc                                                                                            <  1:1199224/140‑1 (MQ=255)

                                           aGTGATGAAGAGAAGGCTGGGTTACGCTATTACGACAATAATTACGTGGAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCttcttcttc                                                                                            <  2:860799/140‑1 (MQ=255)

                                              gatgaAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAg                                                                                         <  1:1419487/140‑1 (MQ=255)

                                               atgaAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGCTCATCTTCGCTTAGTTGCTGAATTAAGTTTACCGCTTCTTCTTCCAgg                                                                                        >  2:730631/1‑140 (MQ=255)

                                               atgaAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAgg                                                                                        >  1:2150179/1‑140 (MQ=255)

                                                tgaAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGt                                                                                       <  2:556421/140‑1 (MQ=255)

                                                   agagAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTccc                                                                                    >  2:600583/1‑140 (MQ=255)

                                                      gaAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGc                                                                                 >  1:567716/1‑140 (MQ=255)

                                                          gCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATg                                                                             >  1:315849/1‑140 (MQ=255)

                                                               gTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTCCCTGAATTGAGTATGCTGCTTCTTCATCCAGATCCCAGCCATGCTGAc                                                                        >  2:1189362/1‑140 (MQ=255)

                                                                  aCGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGaa                                                                     <  1:589053/140‑1 (MQ=255)

                                                                  aCGCTATTCCGCGAATAATTACATGCAATCGAAGGACCAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGaa                                                                     <  1:730631/140‑1 (MQ=255)

                                                                        ttCCGCGAATAATTACGTGAAATGGACGGACGAATATCTGGATCAGGGTAACCAGACCCCGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCg                                                               >  2:603704/1‑140 (MQ=255)

                                                                        ttCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCg                                                               >  1:1665824/1‑140 (MQ=255)

                                                                        ttCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCg                                                               <  2:2150179/140‑1 (MQ=255)

                                                                        ttCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCg                                                               <  2:2075079/140‑1 (MQ=255)

                                                                        ttCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCg                                                               >  1:243270/1‑140 (MQ=255)

                                                                        ttCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCg                                                               >  2:1567518/1‑140 (MQ=255)

                                                                        ttCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTACAGATCGCAGCGAGGTTGACGAAAACTCg                                                               >  1:716706/1‑140 (MQ=255)

                                                                         tCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAAc                                                                  >  2:1570779/1‑136 (MQ=255)

                                                                         tCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGc                                                              >  1:1423612/1‑140 (MQ=255)

                                                                          ccGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCt                                                             >  2:1422861/1‑140 (MQ=255)

                                                                          ccGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCt                                                             <  1:2392706/140‑1 (MQ=255)

                                                                          ccGCGAATAATTACGCGAAATGTAACGACTAATATCTTGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCt                                                             <  1:712714/140‑1 (MQ=255)

                                                                             cgAATAATTACGTGACATGGAATGAAGAAAATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAAc                                                          <  1:879519/140‑1 (MQ=255)

                                                                             cgAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAAc                                                          >  1:2443627/1‑140 (MQ=255)

                                                                               aataatTAGGTGAAATTAAAGGCCGAATATCGGGAGCAGGGTAACCAGGCCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCa                                                        <  2:1182651/140‑1 (MQ=255)

                                                                               aataatTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCa                                                        <  2:48303/140‑1 (MQ=255)

                                                                               aataatTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCa                                                        <  1:2006621/140‑1 (MQ=255)

                                                                               aataatTACGTGAAATGGAAGGACGAATATCTGGATCAGGCTAACCAGACCCAGCCTCGATCATCTTGGCTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAGCTCGCTAACCa                                                        <  2:1652467/140‑1 (MQ=255)

                                                                                    ttACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGtt                                                   <  2:51674/140‑1 (MQ=255)

                                                                                    ttACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGtt                                                   <  1:2136039/140‑1 (MQ=255)

                                                                                      aCGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCt                                                 <  2:1856648/140‑1 (MQ=255)

                                                                                        gTGAAATGGAAGGACGATTATCAGGATCAGGATAACCAGGCCCAGCCTAGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTgg                                               <  2:770955/140‑1 (MQ=255)

                                                                                         tGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGc                                              <  2:243270/140‑1 (MQ=255)

                                                                                          gAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCa                                                        >  1:465583/1‑129 (MQ=255)

                                                                                            aaTGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTGACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTa                                           >  2:2529919/1‑140 (MQ=255)

                                                                                            aaTGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCTCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTa                                           >  1:474622/1‑140 (MQ=255)

                                                                                            aaTGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGt                                                    <  1:1396531/131‑1 (MQ=255)

                                                                                                 aaGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTcc                                      <  2:1959243/140‑1 (MQ=255)

                                                                                                 aaGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTcc                                      <  1:197603/140‑1 (MQ=255)

                                                                                                   ggACGAATATCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAg                                    >  1:279094/1‑140 (MQ=255)

                                                                                                          tatCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCg                             >  2:477177/1‑140 (MQ=255)

                                                                                                           atCTGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCTTATCTCCAGTAAAGCGa                            <  2:1285614/140‑1 (MQ=255)

                                                                                                              tGGATCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATAc                         >  1:228894/1‑140 (MQ=255)

                                                                                                                  tCAGGGTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCa                     >  2:263489/1‑140 (MQ=255)

                                                                                                                     gggTAACCAGACCCACCCTCGACCACCTTAACTTTGTCCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGAc                  <  1:742535/140‑1 (MQ=255)

                                                                                                                      ggTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGa                            <  2:2469762/129‑1 (MQ=255)

                                                                                                                      ggTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGa                            >  2:1779490/1‑129 (MQ=255)

                                                                                                                      ggTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGa                            >  1:2469762/1‑129 (MQ=255)

                                                                                                                      ggTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGAct                 >  2:1648276/1‑140 (MQ=255)

                                                                                                                      ggTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGAct                 >  1:187272/1‑140 (MQ=255)

                                                                                                                      ggTAACCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGAct                 <  2:1983633/140‑1 (MQ=255)

                                                                                                                          aCCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGAct                 <  2:2445626/136‑1 (MQ=255)

                                                                                                                          aCCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCg             >  2:498807/1‑140 (MQ=255)

                                                                                                                          aCCAGACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCg             >  1:196434/1‑140 (MQ=255)

                                                                                                                              gACCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCag         >  1:1661795/1‑140 (MQ=255)

                                                                                                                               aCCCAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCaga        >  1:2390457/1‑140 (MQ=255)

                                                                                                                                cccAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCagag       >  2:1674905/1‑140 (MQ=255)

                                                                                                                                cccAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCagag       >  2:142095/1‑140 (MQ=255)

                                                                                                                                cccAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTAACCCCAGTAAAGCGATACCCCAGACTCTCGCCagag       >  2:1120405/1‑140 (MQ=255)

                                                                                                                                 ccAGCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCAgaga      <  1:1674905/140‑1 (MQ=255)

                                                                                                                                 ccAGCCTCGATCATCTTCACTTAGTTCCTTAATTAAGATTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCAgaga      <  1:1120405/140‑1 (MQ=255)

                                                                                                                                    gCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGActc                <  1:949270/127‑1 (MQ=255)

                                                                                                                                    gCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCAGAGATcg   >  1:2460217/1‑140 (MQ=255)

                                                                                                                                    gCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCAGAGATcg   <  1:1835460/140‑1 (MQ=255)

                                                                                                                                    gCCTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTAACTCCAGTAAAGCGATACGCCAGActc                >  2:949270/1‑127 (MQ=255)

                                                                                                                                     ccTCGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTATGGCGTATATCCAGTAAAGCGATACCCCAGCCTCTCGCCAGAGATcgc  >  2:995175/1‑140 (MQ=255)

ATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTGGGTTACGCTATTCCGCGAATAATTACGTGAAATGGAAGGACGAATATCTGGATCAGGGTAACCAGACCCAGCCTTGATCATCTTCACTTTGTTCCTGAATTAAGTTTTCCGCTTCTTCTTCCAGGTCCCAGCGATGCTGACGAAAACTCGCTAACCACTGTTCTGGCGTATCTCCAGTAAAGCGATACGCCAGACTCTCGCCAGAGATCGC  >  NC_000913/3429652‑3429924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A