Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A27 F60 I1 R1	22	95.6	3256924	99.1%	3227611	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,527,285	T→G	I85L (ATC→CTC)	sgcE ←	KpLE2 phage‑like element; putative epimerase SgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,527,285	0	T	G	100.0%	290.6 / NA	81	I85L (ATC→CTC)	sgcE	KpLE2 phage‑like element; putative epimerase SgcE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (40/41); total (40/41)

TCCAGCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAA  >  NC_000913/4527149‑4527419

tCCAGCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGGTTACGTTTTCGAAGGATAGTCCAGCCGTTTAGCATGAGCGAAGAGCCa                                                                                                                                     >  2:508165/1‑140 (MQ=255)

tCCAGCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGGTAAAGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCa                                                                                                                                     >  2:917194/1‑140 (MQ=255)

 ccAGCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTAT‑GCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcg                                                                                                                                   <  1:1399820/140‑1 (MQ=255)

  cAGCTCAGACGCCAGTTATCGCTAGCCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcg                                                                                                                                   <  1:611767/140‑1 (MQ=255)

  cAGCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcg                                                                                                                                   <  1:216005/140‑1 (MQ=255)

  cAGCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcg                                                                                                                                   <  2:1413535/140‑1 (MQ=255)

   aGCTCAGACGCCAGATATCGCCTGGCGTCAATCGGGGCTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcgc                                                                                                                                  <  1:414965/140‑1 (MQ=255)

    gCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcgcg                                                                                                                                 <  2:57789/140‑1 (MQ=255)

    gCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcgcg                                                                                                                                 <  2:394414/140‑1 (MQ=255)

    gCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcgcg                                                                                                                                 >  1:1519375/1‑140 (MQ=255)

    gCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCAcgcg                                                                                                                                 <  2:90221/140‑1 (MQ=255)

     cTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCgg                                                                                                                                >  2:1086276/1‑140 (MQ=255)

     cTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCgg                                                                                                                                >  2:1273528/1‑140 (MQ=255)

       cAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGt                                                                                                                              <  1:1136620/140‑1 (MQ=255)

        aGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTc                                                                                                                             <  2:848241/140‑1 (MQ=255)

                     cGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTcagccag                                                                                                                <  2:232271/140‑1 (MQ=255)

                      gCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCGAGCGGTTCTTCATGGAGGAAGAGCCACCCGAGTCTGATTTcagccagc                                                                                                               >  2:665869/1‑140 (MQ=255)

                          ggCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGt                                                                                                           >  1:929829/1‑140 (MQ=255)

                          ggCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGt                                                                                                           <  1:1273528/140‑1 (MQ=255)

                          ggCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGt                                                                                                           <  1:1086276/140‑1 (MQ=255)

                            cGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTa                                                                                                         >  1:955034/1‑140 (MQ=255)

                              tCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTaaa                                                                                                       >  1:880279/1‑140 (MQ=255)

                              tCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTaaa                                                                                                       >  1:846921/1‑140 (MQ=255)

                                gATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAAcc                                                                                                     >  2:1244238/1‑140 (MQ=255)

                                    ggggTTGCCGGATTAATGACCAATCCGGCTCGTACGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAgcg                                                                                                 <  2:750142/140‑1 (MQ=255)

                                    ggggTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAgcg                                                                                                 <  2:846921/140‑1 (MQ=255)

                                     gggTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTTCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCCCGCGGGTCTGATTTCAGCCAGCGCGTTAAACACgcgc                                                                                                >  2:87320/1‑140 (MQ=255)

                                       gTTGCCGGATTAAATACCAGTCTGGCTCGTGTGCCCGTACGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCtg                                                                                              <  2:509198/140‑1 (MQ=255)

                                         tGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCtgtg                                                                                            >  1:1433944/1‑140 (MQ=255)

                                               aTTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCggg                                                                                      <  1:1356078/140‑1 (MQ=255)

                                                ttAAATACCAGTCCGACTCGTGCGCCGGTAGGGCGTATCACAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGc                                                                                     <  2:771924/140‑1 (MQ=255)

                                                           tCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaat                                                                          >  1:199227/1‑140 (MQ=255)

                                                           tCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGAGCGTTAATCCATCGACGTGGCCGGGCAAGCatgaaat                                                                          >  1:517028/1‑140 (MQ=255)

                                                           tCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGGCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaat                                                                          >  1:1338434/1‑140 (MQ=255)

                                                             cGGCTCGTGCGCCGGTATGGTGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatga                                                                        >  2:1461232/1‑140 (MQ=255)

                                                             cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCggg                                                                                      >  2:1429994/1‑126 (MQ=255)

                                                             cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCggg                                                                                      <  1:1429994/126‑1 (MQ=255)

                                                             cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatga                                                                        >  1:889716/1‑140 (MQ=255)

                                                             cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatga                                                                        >  2:295209/1‑140 (MQ=255)

                                                             cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatga                                                                        <  2:199227/140‑1 (MQ=255)

                                                             cGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGGCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatga                                                                        >  2:1302482/1‑140 (MQ=255)

                                                              ggCTCGTGCGCCGGTATTGCGGATCTCAGTTTACGTTTCTGAAGGATATTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatgaa                                                                       <  2:712441/140‑1 (MQ=255)

                                                              ggCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCatgaaatgaa                                                                       <  1:126163/140‑1 (MQ=255)

                                                                   gTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGAc                                                                  <  1:1011921/140‑1 (MQ=255)

                                                                   gTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGAc                                                                  <  2:1338434/140‑1 (MQ=255)

                                                                    tGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGGTAGTCCAGCGGTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAGACCCGAGCGGTGGCCGGGGAAGCATGAAATGAAAGGACa                                                                 >  2:779398/1‑140 (MQ=255)

                                                                      cgcCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCGGCGCTGTTGCCGGGAAAGCATTAAATGAAAGGACAAg                                                               >  1:583320/1‑140 (MQ=255)

                                                                             aTGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAg                                                        >  2:1467584/1‑140 (MQ=255)

                                                                                   gATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCgtct                                                  >  1:814492/1‑140 (MQ=255)

                                                                                    aTCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCACTGTGGCCGGGCAAGCGTGAAATGAAAGGAGAAGGGATGAGGCggctg                                                 >  2:604498/1‑140 (MQ=255)

                                                                                     tctcAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCgtctgt                                                <  2:1519375/140‑1 (MQ=255)

                                                                                     tctcAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGCGCAAGAATGAAATGAAAGGACGAAGGATGAGGCgtctgt                                                >  2:724922/1‑140 (MQ=255)

                                                                                      ctcAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCgtctgtc                                               <  1:1302482/140‑1 (MQ=255)

                                                                                      ctcAGTTAACGTTTCCGAAGGATAGTCCAGCGCTTCTGCATGAACGAAGAGCCACGCGGGGCTGATTTCTGCCCGCGCGTTAAACCAGCGCGGTGGCCGGGCAAGCATGAAATGAAAAGACAAGGGATGAGGCggccgtc                                               >  2:1583615/1‑140 (MQ=255)

                                                                                      ctcAGTTAACGTTTCAGAAGGAAAGCCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCgtctgtc                                               <  1:604498/140‑1 (MQ=255)

                                                                                           ttAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCg                                          <  1:1583615/140‑1 (MQ=255)

                                                                                           ttAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCg                                          >  2:941877/1‑140 (MQ=255)

                                                                                              aCGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACg                                       >  2:1741/1‑140 (MQ=255)

                                                                                                gTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGc                                     >  2:388143/1‑140 (MQ=255)

                                                                                                   tCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGACGCGTCTGTCTGGCGACGGCCTg                                  >  2:720729/1‑140 (MQ=255)

                                                                                                     cGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCCTGAAATGAAAGGGCAATGGATGAGGCGTCTTTCTGGCGACGGCCTGCa                                >  2:550516/1‑140 (MQ=255)

                                                                                                     cGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCa                                <  2:955034/140‑1 (MQ=255)

                                                                                                        aGGATAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGATAAGGGATGAGGGGTATGTCTGGCGACGGCCTGCACCg                             >  1:557368/1‑140 (MQ=255)

                                                                                                           aTAGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGttt                          <  2:814492/140‑1 (MQ=255)

                                                                                                             aGTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTc                        <  2:1433944/140‑1 (MQ=255)

                                                                                                              gTCCAGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCa                       >  2:1506331/1‑140 (MQ=255)

                                                                                                                 cAGCGTTTCTGCATGAACGAAGCGCCACGCGTGTCGGATATCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATcc                    <  2:557368/140‑1 (MQ=255)

                                                                                                                  aGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATccc                   <  1:1244238/140‑1 (MQ=255)

                                                                                                                  aGCGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATccc                   <  1:295209/140‑1 (MQ=255)

                                                                                                                    cGTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCaa                 >  2:921160/1‑140 (MQ=255)

                                                                                                                     gTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATcc                    >  1:1206123/1‑136 (MQ=255)

                                                                                                                     gTTTCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATcc                    <  2:1206123/136‑1 (MQ=255)

                                                                                                                      tttCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAAc               >  2:1475204/1‑140 (MQ=255)

                                                                                                                      tttCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGACTGTCTGTCGATGGTCTGCACCGTTTTCATCCCAAAc               >  1:549151/1‑140 (MQ=255)

                                                                                                                       ttCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACg              <  1:1506331/140‑1 (MQ=255)

                                                                                                                        tCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGt             >  1:1067453/1‑140 (MQ=255)

                                                                                                                        tCTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGt             >  2:1134698/1‑140 (MQ=255)

                                                                                                                         cTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTa            >  2:1585975/1‑140 (MQ=255)

                                                                                                                         cTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTa            >  2:158337/1‑140 (MQ=255)

                                                                                                                         cTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTa            <  1:921160/140‑1 (MQ=255)

                                                                                                                         cTGCATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTa            <  1:1134698/140‑1 (MQ=255)

                                                                                                                            cATGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAAtg         <  1:917194/140‑1 (MQ=255)

                                                                                                                              tGAACGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGAATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGACGACGGCGTGCACCGTTTTCAGCCCAAACGTAAtgtt       >  1:522783/1‑140 (MQ=255)

                                                                                                                                aaCGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATccc                   <  2:1231836/126‑1 (MQ=255)

                                                                                                                                aaCGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATccc                   >  1:1231836/1‑126 (MQ=255)

                                                                                                                                 aCGAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATgttgtt    >  1:88086/1‑140 (MQ=255)

                                                                                                                                   gAAGAGCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTaa  <  1:1461232/140‑1 (MQ=255)

TCCAGCTCAGACGCCAGATATCGCCAGGCGTCGATCGGGGTTGCCGGATTAAATACCAGTCCGGCTCGTGCGCCGGTATGGCGGATCTCAGTTAACGTTTCCGAAGGATAGTCCAGCGTTTCTGCATGAACGAAGATCCACGCGGGTCTGATTTCAGCCAGCGCGTTAAACCAGCGCTGTGGCCGGGCAAGCATGAAATGAAAGGACAAGGGATGAGGCGTCTGTCTGGCGACGGCCTGCACCGTTTTCATCCCAAACGTAATGTTGTTAA  >  NC_000913/4527149‑4527419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A