Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A29 F60 I1 R1	18	97.3	3347178	98.9%	3310359	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,532,949	C→T	A79T (GCG→ACG)	yjhP ←	KpLE2 phage‑like element; putative methyltransferase YjhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,532,949	0	C	T	100.0%	168.0 / NA	50	A79T (GCG→ACG)	yjhP	KpLE2 phage‑like element; putative methyltransferase YjhP
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/26); total (24/26)

TCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCG  >  NC_000913/4532813‑4533077

tcctccGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCcgtgcg                                                                                                                               >  1:1390942/1‑140 (MQ=255)

     cGCCCCGGTAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCt                                                                                                                          >  1:368727/1‑140 (MQ=255)

     cGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCt                                                                                                                          >  1:1250042/1‑140 (MQ=255)

              aaaCCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTgcg                                                                                                                 <  2:1250042/140‑1 (MQ=255)

                    aCCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGa                                                                                                           >  1:164902/1‑140 (MQ=255)

                    aCCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGa                                                                                                           >  2:1532945/1‑140 (MQ=255)

                       ggCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAaga                                                                                                        <  2:368727/140‑1 (MQ=255)

                          aaTCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCg                                                                                                     <  1:280164/140‑1 (MQ=255)

                            tCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCt                                                                                                                    <  1:1465363/123‑1 (MQ=255)

                            tCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCt                                                                                                                    >  2:1465363/1‑123 (MQ=255)

                               aTGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTc                                                                                                <  1:256463/140‑1 (MQ=255)

                                tGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCa                                                                                               >  2:1240164/1‑140 (MQ=255)

                                tGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCa                                                                                               >  2:1282529/1‑140 (MQ=255)

                                  tCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATg                                                                                             <  1:1242004/140‑1 (MQ=255)

                                   cgcgCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAagag                                                                                                       <  1:1355056/129‑1 (MQ=255)

                                   cgcgCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAagag                                                                                                       >  2:1355056/1‑129 (MQ=255)

                                       ccGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGAt                                                                                        <  1:1489935/140‑1 (MQ=255)

                                        cGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGt                                                                                            <  2:389945/135‑1 (MQ=255)

                                        cGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGt                                                                                            >  1:389945/1‑135 (MQ=255)

                                          aCGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGcc                                                                                     <  1:1316105/140‑1 (MQ=255)

                                          aCGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGcc                                                                                     >  2:68838/1‑140 (MQ=255)

                                                      aCGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAAt                                                                         <  1:1616186/140‑1 (MQ=255)

                                                          cacaTTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCa                                                                     <  2:1057888/140‑1 (MQ=255)

                                                          cacaTTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCa                                                                     >  1:407817/1‑140 (MQ=255)

                                                          cacaTTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCa                                                                     <  2:407817/140‑1 (MQ=255)

                                                          cacaTTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCa                                                                     >  1:1057888/1‑140 (MQ=255)

                                                          cacaTTTTTCATTTGCGACGTAACCGGCCACATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCa                                                                     >  2:1191653/1‑140 (MQ=255)

                                                           acaTTTTTCATTTGCCACGTAACCGCCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCAt                                                                    <  1:755881/140‑1 (MQ=255)

                                                            caTTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATg                                                                   <  1:27010/140‑1 (MQ=255)

                                                                  tCATTTGCGACGTAACCGGCCGTATCGTTATTAATAAAAAGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCt                                                             <  2:656504/140‑1 (MQ=255)

                                                                  tCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCt                                                             <  1:158168/140‑1 (MQ=255)

                                                                  tCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCt                                                             <  1:1532945/140‑1 (MQ=255)

                                                                      ttGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGcc                                                         >  1:1603856/1‑140 (MQ=255)

                                                                       tGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGccc                                                        <  2:1603856/140‑1 (MQ=255)

                                                                          gACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAgg                                                     <  1:230272/140‑1 (MQ=255)

                                                                                        cATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGTTGCAGAGCATCTCg                                       >  2:311749/1‑140 (MQ=255)

                                                                                          aggTTATGAATAAAACGTACGCATTAGCTTACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGcc                                     <  1:775101/138‑1 (MQ=255)

                                                                                              tATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATACCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAg                                 <  1:1240164/140‑1 (MQ=255)

                                                                                                  aaTAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATGCCTAGCCCAGGTGCAGACCATCTCGCCCGAGCCGc                             >  2:512077/1‑140 (MQ=255)

                                                                                                      aaaTGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGcc                         <  2:164902/140‑1 (MQ=255)

                                                                                                      aaaTGTACGCGTTCGCTGACGCCGACTTCTTCCGTGCCACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGcc                         <  1:521784/140‑1 (MQ=255)

                                                                                                       aaTGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATCCCATGATCGCTGGCCCGGGTGCAGACCATCCGGCCCGAGCCGCTGcct                        >  2:775101/1‑139 (MQ=255)

                                                                                                         tGTACGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAg                      <  1:1191653/140‑1 (MQ=255)

                                                                                                            aCGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTc                   >  2:266066/1‑140 (MQ=255)

                                                                                                            aCGCGTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTc                   >  1:820234/1‑140 (MQ=255)

                                                                                                             cgcgTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGGTCCCTGGCCCAGCTGCAGAGCATCTAGCCCAAGCCGCTGCCGAGGTCa                  >  2:582979/1‑140 (MQ=255)

                                                                                                             cgcgTTCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCa                  >  2:320402/1‑140 (MQ=255)

                                                                                                                 ttCGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCTTGGCCCAGCTGCAGAGAATCTCGCCTGAGCAGCTGCCGAGGTCAAGaa              >  2:521784/1‑140 (MQ=255)

                                                                                                                   cGCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATg            >  2:953625/1‑140 (MQ=255)

                                                                                                                    gCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGc           <  1:205277/140‑1 (MQ=255)

                                                                                                                    gCTGACGCCGAGTTCTTCCGTGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGc           >  1:1174577/1‑140 (MQ=255)

                                                                                                                             gAGTTCTTCCGTGCGTCGCTTTTCCGGCGCGGTGAAGCGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCg  <  1:582979/140‑1 (MQ=255)

TCCTCCGCCCCGGCAAACCCACCGGCAATCCATGTCGCGCCGACGCAGGCCGCCACGTCACATTTTTCATTTGCGACGTAACCGGCCGCATCGTTATGAATAAAATGTACGCGTTCGCTGACGCCGAGTTCTTCCGCGCGACGCTTTGCCTGCGCGGTGAAGAGCGAACTCATGTCGATGCCAGTCCCCGTAATGCCATGATCCCTGGCCCAGGTGCAGAGCATCTCGCCCGAGCCGCTGCCGAGGTCAAGAATGCGGGTGCCCG  >  NC_000913/4532813‑4533077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A