Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A38 F60 I1 R1	15	18.7	632828	98.9%	625866	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,289	G→A	A116T (GCG→ACG)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,289	0	G	A	100.0%	121.1 / NA	35	A116T (GCG→ACG)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/13); total (22/13)

CCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCT  >  NC_000913/3240162‑3240419

ccaGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATAttt                                                                                                                        >  2:185140/1‑140 (MQ=255)

   gCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTcgc                                                                                                                     <  2:13302/140‑1 (MQ=255)

     aCGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTcgccg                                                                                                                   <  2:242033/140‑1 (MQ=255)

        ggCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGccgccg                                                                                                                >  1:224038/1‑140 (MQ=255)

                     tatCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCg                                                                                                   >  1:229506/1‑140 (MQ=255)

                            ccTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGAt                                                                                            <  1:185140/140‑1 (MQ=255)

                                    gTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGc                                                                                    <  2:204138/140‑1 (MQ=255)

                                           tatctaCTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTg                                                                                  >  2:69785/1‑135 (MQ=255)

                                           tatctaCTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTg                                                                                  <  1:69785/135‑1 (MQ=255)

                                                    ggCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTa                                                                               >  1:25127/1‑129 (MQ=255)

                                                    ggCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTa                                                                               <  2:25127/129‑1 (MQ=255)

                                                    ggCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGt                                                                    >  1:301558/1‑140 (MQ=255)

                                                    ggCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGt                                                                    >  2:269877/1‑140 (MQ=255)

                                                     gCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAAAGAGCATGGtt                                                                   >  1:216415/1‑140 (MQ=255)

                                                      cACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGttt                                                                  >  1:222250/1‑140 (MQ=255)

                                                       aCCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTc                                                                 >  2:238973/1‑140 (MQ=255)

                                                       aCCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTc                                                                 >  1:291642/1‑140 (MQ=255)

                                                              ctgctgCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAAcccc                                                          >  2:286786/1‑140 (MQ=255)

                                                                   gctCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGa                                                     >  2:50875/1‑140 (MQ=255)

                                                                    ctctGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTcc                                                                >  2:302362/1‑128 (MQ=255)

                                                                    ctctGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTcc                                                                <  1:302362/128‑1 (MQ=255)

                                                                     tctGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGAcg                                                   <  1:269877/140‑1 (MQ=255)

                                                                                   tCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGa                                          >  2:259647/1‑135 (MQ=255)

                                                                                   tCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGa                                          <  1:259647/135‑1 (MQ=255)

                                                                                     ttCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTaa                                   >  1:297808/1‑140 (MQ=255)

                                                                                           tttGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACAt                             <  2:222250/140‑1 (MQ=255)

                                                                                                 ttcccttcTACCCTGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGAt                       <  1:238973/140‑1 (MQ=255)

                                                                                                              tGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCg          >  1:215281/1‑140 (MQ=255)

                                                                                                              tGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCg          >  2:10367/1‑140 (MQ=255)

                                                                                                              tGCATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCg          >  1:41162/1‑140 (MQ=255)

                                                                                                                cATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGAt        >  2:224004/1‑140 (MQ=255)

                                                                                                                cATTTATCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGAt        >  2:177266/1‑140 (MQ=255)

                                                                                                                     aTCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGcc   >  1:91887/1‑140 (MQ=255)

                                                                                                                      tCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCt  <  2:224038/140‑1 (MQ=255)

                                                                                                                      tCCAGTAGCACGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCt  <  1:50875/140‑1 (MQ=255)

CCAGCACGGGCAGAAAACCAATATCCGCCCTATTTTGTTCCTCTATCTACTGGGCACCTTCTCTGCTGCTCTGGCCGCAGTAGTCTTCAGCTTTGCCTTCCCTTCTACCCTGCATTTATCCAGTAGCGCGGGTGATATTTCGCCGCCGTCAGGCATTGTCGAAGTGATGCGCGGGCTGGTAATGAGCATGGTTTCCAACCCCATCGACGCGCTGCTGAAAGGTAACTACATCGGGATTCTGGTGTGGGCGATCGGCCT  >  NC_000913/3240162‑3240419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A