Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A31 F60 I1 R1 15 64.1 2187582 98.8% 2161331 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,592,741 C→A A233S (GCG→TCG)  livF ← branched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,592,7410CA100.0% 263.4 / NA 80A233S (GCG→TCG) livFbranched chain amino acid/phenylalanine ABC transporter ATP binding subunit LivF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (48/32);  total (48/32)

TGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGCACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGT  >  NC_000913/3592605‑3592873
                                                                                                                                        |                                                                                                                                    
tGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACt                                                                                                                                   >  2:589646/1‑140 (MQ=255)
tGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACG‑GTTATTACCCGCCTAAATACGAACtt                                                                                                                                  >  2:733588/1‑140 (MQ=255)
  cAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTtc                                                                                                                                 >  2:81650/1‑140 (MQ=255)
             tatTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCa                                                                                                                      <  1:589646/140‑1 (MQ=255)
                  ccACTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATGACCCGCCCAAAAACGAACTTCCCACCGCTTCATTCGc                                                                                                                 >  1:344774/1‑140 (MQ=38)
                     cTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCcag                                                                                                              >  1:967032/1‑140 (MQ=255)
                     cTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCcag                                                                                                              >  2:774226/1‑140 (MQ=255)
                     cTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCcag                                                                                                              >  1:429058/1‑140 (MQ=255)
                     cTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCcag                                                                                                              >  2:209382/1‑140 (MQ=255)
                      tCACCCAGTAGGCCTCATAAGCACACCGCATCAGGCACTTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCcagc                                                                                                             <  1:314298/140‑1 (MQ=255)
                             gTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCGCCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCAt                                                                                                      <  1:1002388/140‑1 (MQ=255)
                             gTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCAt                                                                                                      >  2:984675/1‑140 (MQ=255)
                               aGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACG‑GTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCAc                                                                                                   <  1:733588/140‑1 (MQ=255)
                                ggCCTGATAAGCGCACCGCATCAGGTAATCTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCAc                                                                                                   <  1:326248/140‑1 (MQ=255)
                                 gCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCAcc                                                                                                  >  1:247651/1‑140 (MQ=255)
                                 gCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCAc                                                                                                   >  1:1077530/1‑139 (MQ=255)
                                 gCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCAc                                                                                                   <  2:1077530/139‑1 (MQ=255)
                                 gCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACAAACTTCTCACCGCTGCATTCGTCAGCAGCGCATCAcc                                                                                                  >  1:532278/1‑140 (MQ=255)
                                  ccTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTGCCCGCCTAAATACGAACTTCTCACCGCTTCAATCGCCAGCAGCGCATCACCa                                                                                                 <  2:1038872/140‑1 (MQ=255)
                                    tGATAAGCGCAGCGCATCAGGCAATGTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGt                                                                                               >  2:746260/1‑140 (MQ=255)
                                     gATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTa                                                                                              <  1:774226/140‑1 (MQ=255)
                                     gATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTa                                                                                              >  2:676417/1‑140 (MQ=255)
                                        aaGCGCAGCGCATCATGCAATTATAGATTCGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCg                                                                                           <  1:467640/140‑1 (MQ=255)
                                        aaGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTa                                                                                              >  1:597811/1‑137 (MQ=255)
                                        aaGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTa                                                                                              <  2:597811/137‑1 (MQ=255)
                                        aaGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCg                                                                                           <  2:247651/140‑1 (MQ=255)
                                           cgcagcgcaTCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGaa                                                                                        >  2:144747/1‑140 (MQ=255)
                                             cagcgcaTCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAg                                                                                      <  1:209382/140‑1 (MQ=255)
                                             cagcgcaTCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAg                                                                                      <  1:754328/140‑1 (MQ=255)
                                             cagcgcaTCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAg                                                                                      <  1:984675/140‑1 (MQ=255)
                                                cgcaTCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCAc                                                                                   <  1:746260/140‑1 (MQ=255)
                                                cgcaTCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCAc                                                                                   >  2:947826/1‑140 (MQ=255)
                                                                    ttGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCa                                                               <  2:1028271/140‑1 (MQ=255)
                                                                     tGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCAc                                                              >  2:593622/1‑140 (MQ=255)
                                                                      gTCACCTGTCTCAATGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACg                                                             >  1:1050483/1‑140 (MQ=255)
                                                                      gTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCg                                                                         <  2:795048/128‑1 (MQ=255)
                                                                      gTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCg                                                                         >  1:795048/1‑128 (MQ=255)
                                                                              tctcAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCa                                                                  >  1:123407/1‑127 (MQ=255)
                                                                              tctcAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCa                                                                  <  2:123407/127‑1 (MQ=255)
                                                                              tctcAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCcgcg                                                     >  1:63203/1‑140 (MQ=255)
                                                                                tcAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGATAGCACTATATGACCATTTTCCAGCACGTAGCCGCGAt                                                   >  1:463545/1‑140 (MQ=255)
                                                                                    tggAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGc                                               <  2:1050483/139‑1 (MQ=255)
                                                                                        gTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGc                                           >  2:613339/1‑140 (MQ=255)
                                                                                        gTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGc                                           >  1:815602/1‑140 (MQ=255)
                                                                                        gTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGc                                           >  2:154010/1‑140 (MQ=255)
                                                                                         tCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCt                                          >  1:664267/1‑140 (MQ=255)
                                                                                               gACTCCCTACCAATCACCGTGTTATTACCCGCCTAAATACGACCTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAgcg                                    <  2:63203/140‑1 (MQ=255)
                                                                                                aCTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAgcgc                                   >  2:543821/1‑140 (MQ=255)
                                                                                                  tCCCTATCAATCAACTTGTTATTACCCGCTTAAATACAAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCt                                 <  1:426882/140‑1 (MQ=255)
                                                                                                    ccTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTgg                               >  2:143738/1‑140 (MQ=255)
                                                                                                     cTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAgcgc                                   >  2:146131/1‑135 (MQ=255)
                                                                                                     cTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAgcgc                                   <  1:146131/135‑1 (MQ=255)
                                                                                                     cTATCAATCAACGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGt                              <  1:651582/140‑1 (MQ=255)
                                                                                                           atcaaCGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGt                        <  1:676417/140‑1 (MQ=255)
                                                                                                              aaCGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCt                     >  1:614471/1‑140 (MQ=255)
                                                                                                                cGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGc                   >  1:722222/1‑140 (MQ=255)
                                                                                                                cGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGc                   >  1:593179/1‑140 (MQ=255)
                                                                                                                cGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGc                   >  2:155405/1‑140 (MQ=255)
                                                                                                                cGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGAGATGCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGc                   >  2:333450/1‑140 (MQ=255)
                                                                                                                cGTGTTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCAGCGATCCACCAGCTGTAGCGCCTGGTTGGCGTTCTGc                   >  1:453145/1‑140 (MQ=255)
                                                                                                                  tgtTATTACCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAACACGTAGCCGCGATCCACCAGCTTTTGCGCCTGGTTGGCGTTCTGCTc                 >  2:378598/1‑140 (MQ=255)
                                                                                                                    ttattaCCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTcga               >  1:264535/1‑140 (MQ=255)
                                                                                                                     tattaCCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGAACCGCCAGTTTTAGCCCCTGGTGGGCGTTCTGCTcgac              >  1:500840/1‑140 (MQ=255)
                                                                                                                      attaCCCGCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACATAGCCGCGTTCCCCTAACTTTAGCGCCTGGTTGGCGTTCTGCTcgacg             >  2:474692/1‑140 (MQ=255)
                                                                                                                        taCCCGCCTAAATACAAACTTCGCAACGCTTCATTCGGCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACgag           <  1:474692/140‑1 (MQ=255)
                                                                                                                         aCCCGCCTAAATACGAAATTCTTACCGCTTCTTTCGCCACCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACgaga          <  2:429058/140‑1 (MQ=255)
                                                                                                                           ccGCCTAAGTACGAACTACTCACCGCTTCATTCCCCAGCTGCGCATCATCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGaaa        <  2:500840/140‑1 (MQ=255)
                                                                                                                           ccGCCTAAATACGAACTTCTCATCGATTCTTTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGaaa        <  1:428639/140‑1 (MQ=255)
                                                                                                                             gCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACgaga          >  2:888258/1‑136 (MQ=255)
                                                                                                                             gCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACgaga          <  1:888258/136‑1 (MQ=255)
                                                                                                                             gCCTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGa      <  1:144747/140‑1 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCTGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTTTACTCGACGAGaaaaac     >  2:1071755/1‑137 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCGTGGATGGCGTTCTGCTCGACGAGAAAGAt     >  1:381589/1‑140 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGa      >  2:251590/1‑139 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGa      >  1:997838/1‑139 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGa      <  1:251590/139‑1 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGa      <  2:997838/139‑1 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCACCTTCAGCACCTGGTTGGCGTTCTGCTCGACGAGAAAGAt     >  2:428639/1‑140 (MQ=255)
                                                                                                                              ccTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACAT‑GCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATa    >  2:822964/1‑140 (MQ=255)
                                                                                                                               cTAAATACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATa    >  1:707986/1‑140 (MQ=255)
                                                                                                                                 aaaTACGAACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGt  <  1:154010/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGCAAATTCAACATATTGCCACTCACCCAGTAGGCCTGATAAGCGCAGCGCATCAGGCAATTTTACATTTGTCACCTGTCTCAAAGGAGTCTTTTGACTCCCTATCAATCAACGTGTTATTACCCGCCTAAATACGCACTTCTCACCGCTTCATTCGCCAGCAGCGCATCACCAGTATCGGAAAGCACTACATGGCCGTTTTCCAGCACGTAGCCGCGATCCGCCAGCTTTAGCGCCTGGTTGGCGTTCTGCTCGACGAGAAAGATAGT  >  NC_000913/3592605‑3592873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A