Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F60 I1 R1 20 81.1 2806374 99.2% 2783923 139.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,150,664 T→C intergenic (+118/+154) yghA → / ← exbD NADP(+)‑dependent aldehyde reductase/Ton complex subunit ExbD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,150,6640TC100.0% 222.6 / NA 58intergenic (+118/+154)yghA/exbDNADP(+)‑dependent aldehyde reductase/Ton complex subunit ExbD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/35);  total (23/35)

CGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAG  >  NC_000913/3150528‑3150798
                                                                                                                                        |                                                                                                                                      
cggcgAGCATTTAGGTTAAAAATATTCCCGGTTGTGATAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTg                                                                                                                                     <  1:504242/140‑1 (MQ=255)
cggcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTg                                                                                                                                     <  1:945929/140‑1 (MQ=255)
cggcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTg                                                                                                                                     <  1:743875/140‑1 (MQ=255)
cggcgAGCATTTAGGATAAAAACATTCCCGGTTGTGAGAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTg                                                                                                                                     <  1:510629/140‑1 (MQ=38)
  gcgAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTa                                                                                                                                   <  2:902539/140‑1 (MQ=255)
  gcgAGCATTTAGGTTAAAAAAATGCCCGGCTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTa                                                                                                                                   <  1:536602/140‑1 (MQ=255)
     aGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGc                                                                                                                                >  2:1115405/1‑140 (MQ=255)
     aGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGc                                                                                                                                <  2:739362/140‑1 (MQ=255)
          ttAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGAt                                                                                                                           <  1:279393/140‑1 (MQ=255)
                  aaaaaaTGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAg                                                                                                                   >  1:80613/1‑140 (MQ=255)
                       aTGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCAt                                                                                                              <  1:835327/140‑1 (MQ=255)
                        tGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATc                                                                                                             >  1:1151075/1‑140 (MQ=255)
                             ggTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                                                        <  1:39185/140‑1 (MQ=255)
                                tgtgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTg                                                                                                     <  2:80613/140‑1 (MQ=255)
                                 gtgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGa                                                                                                    <  1:691617/140‑1 (MQ=255)
                                  tgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAg                                                                                                   <  2:976605/140‑1 (MQ=255)
                                  tgAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAg                                                                                                   <  1:329672/140‑1 (MQ=255)
                                   gAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGc                                                                                                  <  2:1151075/140‑1 (MQ=255)
                                         cAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAGGCGAAGGGCATCAGGCATTGAGCACCTaa                                                                                            >  1:362385/1‑140 (MQ=255)
                                                gCATCATTGTGAATTAACTCTTCCCCGATGTGGCGAAGACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGa                                                                                     <  1:437921/140‑1 (MQ=25)
                                                    catTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGa                                                                                     <  1:195477/136‑1 (MQ=255)
                                                    catTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGa                                                                                     >  2:195477/1‑136 (MQ=255)
                                                         tgAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTg                                                                            <  2:46311/140‑1 (MQ=255)
                                                         tgAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTg                                                                            >  1:968188/1‑140 (MQ=255)
                                                           aaTTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAACACCTAATGACGGATGCGTCGTGaa                                                                          >  1:446148/1‑140 (MQ=255)
                                                                  tctTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTa                                                                   >  2:1018799/1‑140 (MQ=255)
                                                                    ttACCTGATGTGGCGAATACGCCTTATCCGCATACAAACTCATGAAGACTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                 <  2:362385/140‑1 (MQ=37)
                                                                    ttACCTGATGTGGCGAAAACGCTTTATCCGCTTACCAACGCATGAAGAATCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                 <  2:446148/140‑1 (MQ=37)
                                                                    ttACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGGGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                 <  1:96534/140‑1 (MQ=255)
                                                                    ttACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                 <  1:1293069/140‑1 (MQ=255)
                                                                     tACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATcc                                                                >  2:699794/1‑140 (MQ=255)
                                                                     tACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTAATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATcc                                                                >  2:302886/1‑140 (MQ=255)
                                                                       ccTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCgg                                                              >  1:852154/1‑140 (MQ=255)
                                                                        cTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGc                                                             <  2:338489/140‑1 (MQ=255)
                                                                         tGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGcc                                                            <  1:1354567/140‑1 (MQ=255)
                                                                            tgtgGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                 <  1:1146400/132‑1 (MQ=255)
                                                                            tgtgGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATc                                                                 >  2:1146400/1‑132 (MQ=255)
                                                                            tgtgGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTAc                                                         >  2:118837/1‑140 (MQ=255)
                                                                                gCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAc                                                     >  2:549654/1‑140 (MQ=255)
                                                                                gCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAAc                                                     >  2:873022/1‑140 (MQ=255)
                                                                                 cGAAAACGCCTTATCCGTCTACCAACGCATGAAGAGTCACCATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCTTTATCCGGCCTACAAACg                                                    <  2:547932/140‑1 (MQ=37)
                                                                                 cGAAAACGCCTTATCCGCCTACGAACGCATGTAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACg                                                    <  2:589647/140‑1 (MQ=255)
                                                                                      aCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGa                                               <  1:1115405/140‑1 (MQ=255)
                                                                                      aCGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGa                                               >  1:718380/1‑140 (MQ=255)
                                                                                        gCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAg                                             <  1:1281615/140‑1 (MQ=255)
                                                                                          cTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGAt                                           >  1:848215/1‑140 (MQ=255)
                                                                                           ttATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGAtt                                          <  1:957188/140‑1 (MQ=255)
                                                                                             aTCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCa                                        >  1:946474/1‑140 (MQ=255)
                                                                                                  ccTACAAACGCATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATg                                   >  1:754866/1‑140 (MQ=255)
                                                                                                            cATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGa                         >  2:1308335/1‑140 (MQ=255)
                                                                                                            cATGAAGATTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGa                         <  2:718380/140‑1 (MQ=255)
                                                                                                                 aGATTCAACATGTTGCAATAGAACTAGTACGCCTGATCAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTg                    <  1:358811/140‑1 (MQ=38)
                                                                                                                   aTTCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTa                  <  1:1018799/140‑1 (MQ=255)
                                                                                                                     tCAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAgg                >  2:1174297/1‑140 (MQ=255)
                                                                                                                      cAACATGTTGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGc               <  2:946474/140‑1 (MQ=255)
                                                                                                                             ttGCAATAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCtgatga        >  2:1123956/1‑140 (MQ=255)
                                                                                                                                   tAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAg  <  2:1002005/140‑1 (MQ=255)
                                                                                                                                   tAGATCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAg  >  1:727412/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CGGCGAGCATTTAGGTTAAAAAAATGCCCGGTTGTGAAAAGCAACCGGGCATCATTGTGAATTCACTCTTACCTGATGTGGCGAAAACGCCTTATCCGCCTACAAACGCATGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATAAGCGTAGCGCATCAGGCATTGAGCACCTAATGCCGGATGCGTCGTGAACGCCTTATCCGGCCTACAAACGCACGAAGATTCAACATGTTGCAATAGATTTTGTAGGCCTGATGAGCGTAG  >  NC_000913/3150528‑3150798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A