Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F60 I1 R1 14 51.9 1820130 99.3% 1807389 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,307,710 T→G L30L (CTA→CTC)  alsK ← D‑allose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,307,7100TG98.1% 185.6 / NA 52L30L (CTA→CTC) alsKD‑allose kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (24/27);  minor base C (0/1);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTGCGCGGTCATAA  >  NC_000913/4307576‑4307844
                                                                                                                                      |                                                                                                                                      
aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGGAATCACTCCTGCGGTCTGCTTTTTTTCGCAGTGGAGCGt                                                                                                                                   >  2:372022/1‑140 (MQ=255)
aCCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGt                                                                                                                                   >  1:109779/1‑140 (MQ=255)
 ccAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGtt                                                                                                                                  >  2:377683/1‑140 (MQ=255)
 ccAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGtt                                                                                                                                  <  1:61536/140‑1 (MQ=255)
 ccAGCGCCGGAAATCCCATCACCAGACCATGACAACGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGtt                                                                                                                                  <  2:593908/140‑1 (MQ=255)
  cAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGttt                                                                                                                                 >  1:553802/1‑140 (MQ=255)
     cgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGTAATGACTTCTGCGGGCCGTTTATTTTCGCAGTGGAGCGTTTCAc                                                                                                                              >  1:260736/1‑140 (MQ=255)
     cgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCAc                                                                                                                              >  1:808863/1‑140 (MQ=255)
     cgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCAc                                                                                                                              >  2:147917/1‑140 (MQ=255)
            aaTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTctgc                                                                                                                       <  2:833468/140‑1 (MQ=255)
               cccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTc                                                                                                                                >  2:705161/1‑128 (MQ=255)
               cccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTc                                                                                                                                <  1:705161/128‑1 (MQ=255)
               cccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGt                                                                                                                    <  1:49367/140‑1 (MQ=255)
                     aCCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTATTTCGCACTGGTGCGTTTCACGTTCTGCTGTCCGCAg                                                                                                              >  1:245210/1‑140 (MQ=255)
                      ccAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGa                                                                                                             <  1:744064/140‑1 (MQ=255)
                            cATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAg                                                                                                       <  2:601080/140‑1 (MQ=255)
                            cATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAg                                                                                                       <  1:88321/140‑1 (MQ=255)
                              tGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCg                                                                                                     >  2:348809/1‑140 (MQ=255)
                                 cAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                                                  >  2:510541/1‑140 (MQ=255)
                                 cAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                                                  <  1:147917/140‑1 (MQ=255)
                                    cgagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCTTTTCACCATCTGCTGTCCGCAGACAAAAGCGGATATg                                                                                               >  1:243237/1‑140 (MQ=255)
                                      agcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCTTTTCAGCCCCTGCTGTCCGCACACAAAAGCGGATATGCg                                                                                             >  2:441412/1‑140 (MQ=255)
                                      agcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCg                                                                                             <  1:650827/140‑1 (MQ=255)
                                        cgTTAAAGCGCCTGAGTCGCTCCTCAATCATCTCGTCGACACCCGACACCAGGCCGGGAGTAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGtt                                                                                           <  2:260736/140‑1 (MQ=255)
                                         gTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTg                                                                                          <  2:820914/140‑1 (MQ=255)
                                         gTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGGGGAGCGTTGCACCTTCTGCTGTCCGGAGACAAAAGCGGATATGCGTTg                                                                                          >  2:357820/1‑140 (MQ=255)
                                         gTTAAAGCACCTGAGTCTCTCGCCAATCATTTCACGGATCCCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTg                                                                                          <  2:440308/140‑1 (MQ=255)
                                           tAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGcc                                                                                        >  1:777135/1‑140 (MQ=255)
                                           tAAAGCGCCTGAGTTGCGCGTCAATCATTTCCCCGATACCCGACACCAAGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGcc                                                                                        <  2:296357/140‑1 (MQ=255)
                                                     gAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCAGCTTCTGCTGTCCTCAGCAAAAAGCGAATATCCGTTGCCCTCATATCCa                                                                              >  2:432247/1‑140 (MQ=255)
                                                     gAGTTGCTCGTCAATCATTTCGCCGATACCCGACACC‑GGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCAc                                                                             >  2:179549/1‑140 (MQ=255)
                                                          gCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGccc                                                                         >  2:842053/1‑140 (MQ=255)
                                                          gCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGccc                                                                         >  1:823846/1‑140 (MQ=255)
                                                          gCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGccc                                                                         >  2:81329/1‑140 (MQ=255)
                                                              gTCAATCATTTCGCCAATACCCGACGCCATGCCGGGAACAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTa                                                                     <  1:441412/140‑1 (MQ=255)
                                                                        tCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATg                                                           >  1:635837/1‑140 (MQ=255)
                                                                        tCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATAGGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATg                                                           <  1:348809/140‑1 (MQ=255)
                                                                        tCGCCGATACCCGACACCAGGCCGGGAGCAACGACCTCTCCGGTCCGCCTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATg                                                           <  2:245210/140‑1 (MQ=255)
                                                                             gATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGtt                                                      >  2:80256/1‑140 (MQ=255)
                                                                                   cGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCa                                                <  2:651352/140‑1 (MQ=255)
                                                                                     acacCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATg                                              <  2:777135/140‑1 (MQ=255)
                                                                                                                   tCCGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAAt                <  2:188972/140‑1 (MQ=255)
                                                                                                                     cGCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCt              >  1:209871/1‑140 (MQ=255)
                                                                                                                      gCTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTg             <  1:80256/140‑1 (MQ=255)
                                                                                                                       cTTTTTTTCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTgc            <  2:635837/140‑1 (MQ=255)
                                                                                                                       cTTTTTTTCGCAGTGCTGCGTTTCAGCTTTTGCTGTCGGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACCTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTgc            <  1:432247/140‑1 (MQ=255)
                                                                                                                        tttttttCGCAGTGGAGCGTTTCACCTTCTGATGTTCGCTGACAAAAGTGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTgcg           <  2:243237/140‑1 (MQ=255)
                                                                                                                         ttttttCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTgcgc          <  1:510541/140‑1 (MQ=255)
                                                                                                                         ttttttCGCAGTGGACCGTTTCACCATCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTgcgc          <  1:302270/140‑1 (MQ=255)
                                                                                                                          tttttCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTGcgcg         <  1:842053/140‑1 (MQ=255)
                                                                                                                            tttCGCAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTGCGCGGt       <  1:81329/140‑1 (MQ=255)
                                                                                                                                 cAGTGGAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAACCAGAATCTGCGCGGTCATaa  >  2:787023/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTTTTTGCAGCCAGAATCTGCGCGGTCATAA  >  NC_000913/4307576‑4307844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A