Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F60 I1 R1	18	124.1	4246592	99.1%	4208372	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,244,007	C→T	V357M (GTG→ATG)	malF ←	maltose ABC transporter membrane subunit MalF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,244,007	0	C	T	99.3%	473.7 / NA	136	V357M (GTG→ATG)	malF	maltose ABC transporter membrane subunit MalF
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (71/64); minor base G (1/0); total (72/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

GCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGC  >  NC_000913/4243871‑4244143

gCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGcc                                                                                                                                       >  1:288427/1‑140 (MQ=255)

gCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGAGAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGcc                                                                                                                                       >  2:1735048/1‑140 (MQ=255)

gCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGA‑AACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGAGGTGGTCGGATAGCTGAACCAGGCGGGCTTCATGCCa                                                                                                                                      >  1:695980/1‑140 (MQ=255)

  ttCATACAAATCGTCCGGAATCCCTTTCAGCGAGCGCATGCAGAGGATCATCATGTACGGATATCCCAGCCATGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGGTTCATGCCaa                                                                                                                                     <  2:861317/140‑1 (MQ=255)

   tCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCaaa                                                                                                                                    >  1:2084168/1‑140 (MQ=255)

       aCAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgc                                                                                                                                <  2:21943/140‑1 (MQ=255)

       aCAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgc                                                                                                                                <  2:2021945/140‑1 (MQ=255)

       aCAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgc                                                                                                                                <  2:1469028/140‑1 (MQ=255)

        cAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgcg                                                                                                                               <  2:1492535/140‑1 (MQ=255)

          aaTCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgcgcg                                                                                                                             >  1:256813/1‑140 (MQ=255)

          aaTCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgcgcg                                                                                                                             >  1:1502813/1‑140 (MQ=255)

          aaTCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgcgcg                                                                                                                             >  2:19356/1‑140 (MQ=255)

           aTCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgcgcgc                                                                                                                            <  1:182247/140‑1 (MQ=255)

            tCGTCCGGAATCGCTGTCAGCACGCTCATTCAGAGGACCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCt                                                                                                                           <  1:1032058/140‑1 (MQ=255)

              gTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGATGGTCGGAACGCGGAAGCAGGCGGGATTCAGGCCAAACAGCGCGCTCa                                                                                                                         >  2:589065/1‑140 (MQ=255)

                ccGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgcgcg                                                                                                                             <  2:1957036/134‑1 (MQ=255)

                ccGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAgcgcg                                                                                                                             >  1:1957036/1‑134 (MQ=255)

                ccGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAAc                                                                                                                       <  1:2090723/140‑1 (MQ=255)

                   gAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAAcatc                                                                                                                    >  2:392864/1‑140 (MQ=255)

                         cTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTg                                                                                                              >  1:1148969/1‑140 (MQ=255)

                           ttCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGAt                                                                                                            <  1:1057842/140‑1 (MQ=255)

                             cAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGAttt                                                                                                          <  1:483788/140‑1 (MQ=255)

                                   gCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGa                                                                                                    >  2:95437/1‑140 (MQ=255)

                                   gCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGa                                                                                                    >  1:1512676/1‑140 (MQ=255)

                                       aTGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGct                                                                                                <  2:1840413/140‑1 (MQ=255)

                                        tGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAACCAGCGCGGTCAGCAGCATGTTTATTTCACCGAAGctc                                                                                               >  1:546350/1‑140 (MQ=255)

                                        tGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGctc                                                                                               >  2:1360561/1‑140 (MQ=255)

                                        tGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGctc                                                                                               >  1:1095641/1‑140 (MQ=255)

                                        tGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGctc                                                                                               >  2:1730156/1‑140 (MQ=255)

                                        tGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGctc                                                                                               >  2:1787714/1‑140 (MQ=255)

                                           agagGATCATCATGTACGGATATCCTAGCTAGGTATTAACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTgg                                                                                            <  1:1001928/140‑1 (MQ=255)

                                           agagGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTgg                                                                                            <  1:1360561/140‑1 (MQ=255)

                                           agagGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTgg                                                                                            <  1:1920340/140‑1 (MQ=255)

                                              ggATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCGCCGAAGCTCTGGTTa                                                                                         >  1:868819/1‑140 (MQ=255)

                                               gATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGAttt                                                                                                          >  2:2093581/1‑122 (MQ=255)

                                               gATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTaa                                                                                        >  1:441258/1‑140 (MQ=255)

                                                 tcatcaTGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAaac                                                                                      <  1:5804/140‑1 (MQ=255)

                                                 tcatcaTGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAaac                                                                                      <  1:1342182/140‑1 (MQ=255)

                                                  catcatGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAaaca                                                                                     <  2:1257165/140‑1 (MQ=255)

                                                   atcatGTACGGATAACCCAGCCAGGTATTGACGATAAATAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAaacaa                                                                                    <  1:842369/140‑1 (MQ=255)

                                                       tGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCt                                                                                >  1:231226/1‑140 (MQ=255)

                                                       tGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACAGCATGTTGCTTTGACTGAAGCTCTGGTTAAACAACCCt                                                                                >  1:744264/1‑140 (MQ=255)

                                                         tACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGGGCTCAACAACATGTTGTTTTCACCGAAGCTGTGGTTAAACAACCCttt                                                                              >  2:557279/1‑140 (MQ=255)

                                                         tACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCAGCGTAGCTCTCGTTAAACAACCCttt                                                                              >  1:821506/1‑140 (MQ=255)

                                                         tACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCttt                                                                              >  2:1093454/1‑140 (MQ=255)

                                                          aCGGATAACCCAGCCCGGTATTCACCATACTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTg                                                                             <  1:579069/140‑1 (MQ=255)

                                                             gATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGaaa                                                                          >  1:1226882/1‑140 (MQ=255)

                                                              aTAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTaaa                                                                                       >  1:236005/1‑126 (MQ=255)

                                                              aTAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGaaaa                                                                         >  2:413967/1‑140 (MQ=255)

                                                               tAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAAt                                                                        <  2:288427/140‑1 (MQ=255)

                                                               tAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAAt                                                                        <  1:392864/140‑1 (MQ=255)

                                                                aaCCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGAGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATc                                                                       >  2:1745018/1‑140 (MQ=255)

                                                                 aCCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCa                                                                      <  1:1730156/140‑1 (MQ=255)

                                                                 aCCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCa                                                                      <  1:1787714/140‑1 (MQ=255)

                                                                  cccAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCaa                                                                     >  1:1044162/1‑140 (MQ=255)

                                                                  cccAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCaa                                                                     >  1:1248044/1‑140 (MQ=255)

                                                                  cccAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCaa                                                                     >  1:1083922/1‑140 (MQ=255)

                                                                  cccAGCCAGGGATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCCGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCaa                                                                     >  2:1143156/1‑140 (MQ=255)

                                                                      gccagGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGAtt                                                                 >  1:127960/1‑140 (MQ=255)

                                                                       ccagGTATTGACGATCATTAGCGTCATGCTGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTg                                                                <  1:976926/140‑1 (MQ=255)

                                                                        cagGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGa                                                               >  1:1635924/1‑140 (MQ=255)

                                                                        cagGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGATTAGACGACCCTTGGAAAATCAAGATTGa                                                               >  1:611669/1‑140 (MQ=255)

                                                                           gTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAAt                                                            >  2:1170266/1‑140 (MQ=255)

                                                                            tATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATg                                                           <  2:253410/140‑1 (MQ=255)

                                                                            tATTGACGATAATTACCATCGTCCGTGCGTTGGTCGGGTCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATg                                                           <  1:557279/140‑1 (MQ=255)

                                                                                gACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACg                                                       >  1:1904755/1‑140 (MQ=255)

                                                                                 aCGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGa                                                      <  2:1612143/140‑1 (MQ=255)

                                                                                     tAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGc                                                  >  1:169749/1‑140 (MQ=255)

                                                                                     tAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGc                                                  >  2:1072181/1‑140 (MQ=255)

                                                                                      aaTTAGCATCGTGCGGGGGGTGTTCGGATCGATGACCCAGGCGGGCTTGATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCa                                                 <  2:819499/140‑1 (MQ=255)

                                                                                      aaTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATg                                                           >  1:2023278/1‑130 (MQ=255)

                                                                                      aaTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCa                                                 <  2:1635924/140‑1 (MQ=255)

                                                                                      aaTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCa                                                 <  2:749956/140‑1 (MQ=255)

                                                                                      aaTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCa                                                 >  1:1646682/1‑140 (MQ=255)

                                                                                      aaTTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCa                                                 <  2:2084168/140‑1 (MQ=255)

                                                                                          aGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACcgc                                             <  1:533462/140‑1 (MQ=255)

                                                                                           gCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACcgcg                                            <  2:149225/140‑1 (MQ=255)

                                                                                           gCATCGTGCGGGCGGTGCTCGGCTCGCTGAAGCAGTCGGCCTTCATGCTAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACcgcg                                            <  2:568243/140‑1 (MQ=255)

                                                                                               cGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTTAAAATCACGATGGATATGAACGATGGCACCGCGTAgg                                        >  1:758617/1‑140 (MQ=255)

                                                                                               cGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAgg                                        <  2:441258/140‑1 (MQ=255)

                                                                                               cGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAgg                                        <  2:1904755/140‑1 (MQ=255)

                                                                                               cGTGCCGGACGTTGTCGTATCGCTGAACCAGGTGCGCTTCATCTGAAACACCGCGTTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAgg                                        <  1:1295869/140‑1 (MQ=255)

                                                                                                gTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGTAAATCAATATTCAACTGAACGCTGGCACCGCGTAggg                                       >  1:883405/1‑140 (MQ=255)

                                                                                                 tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGCTTGTAATGAACGACGGCACCGCGTAGGGc                                      >  1:819499/1‑140 (MQ=255)

                                                                                                 tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACcgcg                                            >  2:1872611/1‑134 (MQ=255)

                                                                                                 tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACcgcg                                            <  1:1872611/134‑1 (MQ=255)

                                                                                                 tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                      >  2:45/1‑140 (MQ=255)

                                                                                                 tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                      <  1:1072948/140‑1 (MQ=255)

                                                                                                 tGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAAAAACCCTTTGAAAATCAAGATTGAAAAGAACAATGGCACCGCGTAGGGc                                      >  2:927290/1‑140 (MQ=255)

                                                                                                 tGCGGGCGGTGGTCGCATCGCTTAAGCAGCCGGGCTTTATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGc                                      <  1:646520/140‑1 (MQ=255)

                                                                                                     ggcggTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACACTTTGAAATTCAGGATTGAAATGAAAGATGTCACCGCGTAGGGCAGaa                                  >  2:677912/1‑140 (MQ=255)

                                                                                                      gcggTGGTCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAAt                                 >  1:1373904/1‑140 (MQ=255)

                                                                                                         gtggtCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATcag                              <  1:153497/140‑1 (MQ=255)

                                                                                                         gtggtCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATcag                              <  2:1076924/140‑1 (MQ=255)

                                                                                                          tggtCGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATcagc                             >  1:165906/1‑140 (MQ=255)

                                                                                                              cGGATCGCTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGa                         <  2:1127712/140‑1 (MQ=255)

                                                                                                                     cTGAACCAGGCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATa                  <  2:253214/140‑1 (MQ=255)

                                                                                                                             ggCGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCtt          <  1:1867949/140‑1 (MQ=255)

                                                                                                                               cGGGCTTCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAAGGATGGCACAGCGTAGGCCAGAATCAGCAGGGCGCGATAGACCGCTTTg        >  2:843898/1‑140 (MQ=255)

                                                                                                                                     tCATGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCgcgc  <  2:1044162/140‑1 (MQ=255)

GCTTCATACAAATCGTCCGGAATCGCTTTCAGCAAGCCCATGCAGAGGATCATCATGTACGGATAACCCAGCCAGGTATTGACGATAATTAGCATCGTGCGGGCGGTGGTCGGATCGCTGAACCAGGCGGGCTTCACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGC  >  NC_000913/4243871‑4244143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A