Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F60 I1 R1 18 124.1 4246592 99.1% 4208372 139.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C96.7% 206.2 / ‑0.3 61intergenic (+586/+56)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base C (28/31);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
*NC_0009134,296,3802.G96.7% 204.9 / ‑0.2 61intergenic (+586/+56)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (28/31);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

AACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAC  >  NC_000913/4296303‑4296505
                                                                                 ||                                                                                                                             
aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAc                                                                   >  2:1087138/1‑140 (MQ=0)
      tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTATAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                             >  2:1315797/1‑140 (MQ=255)
       cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGGCTGATGCGCCGCTTGCGGCGTCTTAACAGGCCTATCTTAACCGTTGGt                                                            >  2:835428/1‑140 (MQ=11)
        ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGtt                                                           >  1:347295/1‑140 (MQ=255)
         tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                          <  2:14034/140‑1 (MQ=255)
          aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTaa                                                         >  1:1883858/1‑140 (MQ=255)
           tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                        <  2:166355/140‑1 (MQ=255)
           tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                        <  2:1149433/140‑1 (MQ=255)
           tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt                                                        <  1:62767/140‑1 (MQ=255)
              ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt                                                     <  2:1900166/140‑1 (MQ=255)
               gCCTACGCCAGACAGCGCAATAGCCAGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttttt                                                    >  2:1459700/1‑140 (MQ=255)
                 cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                   >  1:1600227/1‑140 (MQ=14)
                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                <  1:1087138/140‑1 (MQ=255)
                   aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGACTATCTTAACCATTGGTTAATTTTTCaaa                                                <  2:1469230/140‑1 (MQ=14)
                    cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                               >  2:1869465/1‑140 (MQ=255)
                    cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                               >  1:1849590/1‑140 (MQ=255)
                    cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCGTGCCGCGTGTTAGCAGGCCTATCTTAACCGGTGGTTAATTTTTCAAAc                                               >  2:613832/1‑140 (MQ=14)
                     gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAct                                              >  2:1367431/1‑140 (MQ=255)
                         gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                               <  1:1166989/135‑1 (MQ=255)
                         gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                               >  2:1166989/1‑135 (MQ=255)
                         gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  2:391721/1‑140 (MQ=255)
                         gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  2:1987360/1‑140 (MQ=255)
                         gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                          >  1:139432/1‑140 (MQ=255)
                          aCAGCGCAATAGCCTGATTTAGTGTGATTATGTAGGTGGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca                                         <  1:835428/140‑1 (MQ=17)
                            aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTcaca                                       <  1:1791086/140‑1 (MQ=255)
                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACTGTTGGTAAATTTTTCAAACTCTTCACAcc                                     >  1:694668/1‑140 (MQ=17)
                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACTGTTAGTAAATTTTACAAACTCTTCACAcc                                     >  1:747702/1‑140 (MQ=14)
                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     >  2:1954450/1‑140 (MQ=255)
                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  2:1883858/140‑1 (MQ=255)
                              cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACAGTTTGTGAATTTTGCAAACTCTTCACAcc                                     >  1:614372/1‑140 (MQ=14)
                               gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                    <  1:433893/140‑1 (MQ=255)
                               gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                    <  2:1455935/140‑1 (MQ=255)
                                cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCg                                   <  2:192831/140‑1 (MQ=255)
                                   tAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa                                >  1:363967/1‑140 (MQ=255)
                                   tAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa                                >  2:1757069/1‑140 (MQ=255)
                                      ccTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                             <  1:1954450/140‑1 (MQ=255)
                                       cTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaa                            <  2:363967/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:1821928/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:1278187/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:1459700/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:1987360/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           >  1:1478078/1‑140 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:1608008/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:1869465/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  2:1414033/140‑1 (MQ=255)
                                        tGATTTAGCGTGATTCTGTAGGTCGCAT‑ATGGCGTTTCCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:597487/140‑1 (MQ=18)
                                        tGATTTAGCGTGATTCTGTAGGGCGCAT‑AAGGCGTTTCCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  1:613832/140‑1 (MQ=18)
                                         gATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                          >  2:1984935/1‑140 (MQ=255)
                                             tAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           >  1:28193/1‑135 (MQ=255)
                                             tAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                           <  2:28193/135‑1 (MQ=255)
                                             tAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                      <  1:1190449/140‑1 (MQ=255)
                                                cGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTATTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     >  1:415/1‑122 (MQ=18)
                                                cGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTATTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                     <  2:415/122‑1 (MQ=18)
                                                    aTTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa               >  2:377376/1‑140 (MQ=255)
                                                    aTTTTGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa               >  2:1061816/1‑140 (MQ=255)
                                                       ttGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  1:1757069/140‑1 (MQ=255)
                                                       ttGTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  2:1478078/140‑1 (MQ=255)
                                                        tGTAGGTCGGATAAAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt            <  2:1600227/140‑1 (MQ=25)
                                                         gTAGGTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa          <  1:391721/140‑1 (MQ=255)
                                                            ggTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt       <  1:434398/140‑1 (MQ=255)
                                                            ggTCGGAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt       <  1:1707156/140‑1 (MQ=255)
                                                                 gAT‑AAGGCGTTT‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc  >  1:1700828/1‑140 (MQ=255)
                                                                                 ||                                                                                                                             
AACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAT‑AAGGCGTTT‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAC  >  NC_000913/4296303‑4296505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A