Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A28 F60 I1 R1 11 82.5 2813942 99.0% 2785802 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,959,035 G→T D356Y (GAT→TAT)  ilvC → ketol‑acid reductoisomerase (NADP(+))

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,0350GT100.0% 283.5 / NA 83D356Y (GAT→TAT) ilvCketol‑acid reductoisomerase (NADP(+))
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (38/45);  total (38/45)

GAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCGATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCAACACCAT  >  NC_000913/3958900‑3959171
                                                                                                                                       |                                                                                                                                        
gAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATaa                                                                                                                                      <  2:1045807/140‑1 (MQ=255)
  aTTCTCTTCCGGTATGATGGCGTACGGGGGCAACGATCATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAg                                                                                                                                    <  1:453065/140‑1 (MQ=255)
  aTTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAg                                                                                                                                    <  1:47924/140‑1 (MQ=255)
  aTTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAg                                                                                                                                    <  2:351849/140‑1 (MQ=255)
      tctTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGt                                                                                                                                <  1:1171664/140‑1 (MQ=255)
      tctTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGt                                                                                                                                <  2:1114104/140‑1 (MQ=255)
        ttCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATaa                                                                                                                                      <  1:1069665/132‑1 (MQ=255)
        ttCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATaa                                                                                                                                      >  2:1069665/1‑132 (MQ=255)
                      cGGACTGGGCCAACGATGATAACAAACTGCTGACCCGGCTTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATg                                                                                                                <  2:548151/140‑1 (MQ=255)
                        gACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGTAGTACTTATATGAATGCGTACAGATGATTGCGATggg                                                                                                              >  1:659954/1‑139 (MQ=255)
                        gACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTGTATGAATGCGTACTGATGATTGCGATGGt                                                                                                              >  1:516093/1‑140 (MQ=255)
                            gggCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGaaa                                                                                                          >  2:178286/1‑140 (MQ=255)
                             ggCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAg                                                                                                         >  1:340934/1‑140 (MQ=255)
                             ggCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAg                                                                                                         >  2:973446/1‑140 (MQ=255)
                              gCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGc                                                                                                        >  2:100461/1‑140 (MQ=255)
                                   cGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCg                                                                                                   >  2:922424/1‑140 (MQ=255)
                                        atAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGaa                                                                                              >  1:730158/1‑140 (MQ=255)
                                            gAAACTGCTGACCTGGTGTGAAGAGACCGGCCAAAGCGCATTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTgg                                                                                          <  2:659954/140‑1 (MQ=255)
                                            gAAACTGCTGACCTGGCTTGAACAGACCGGCAAATCCGCATTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTgg                                                                                          <  2:516093/140‑1 (MQ=255)
                                            gAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTgg                                                                                          <  2:340934/140‑1 (MQ=255)
                                            gAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTgg                                                                                          <  1:318805/140‑1 (MQ=255)
                                             aaaCTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGc                                                                                         >  2:1081399/1‑140 (MQ=255)
                                                ctgctgACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGtt                                                                                      <  1:1322956/140‑1 (MQ=255)
                                                  gctgACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCg                                                                                    >  1:1006894/1‑140 (MQ=255)
                                                  gctgACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATAAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGACGGTGAAAGCGGGCATTGAACTGGCGTTCg                                                                                    >  1:460662/1‑140 (MQ=255)
                                                   ctgACCTGGCGTGAAGAGACCGTCAAAACCGCGTTTGAACCCGCGCCGTAGTATAAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGa                                                                                   <  2:460662/140‑1 (MQ=255)
                                                   ctgACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGGAAACGGGCGATGAACTGGCGTTCGa                                                                                   >  1:407213/1‑140 (MQ=255)
                                                   ctgACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGa                                                                                   >  2:990283/1‑140 (MQ=255)
                                                   ctgACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGa                                                                                   <  2:1006894/140‑1 (MQ=255)
                                                      aCCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAAc                                                                                >  2:339919/1‑140 (MQ=255)
                                                      aCCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAAc                                                                                >  1:966036/1‑140 (MQ=255)
                                                          ggCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCCATGGTGATAGCGGGCGCTGAACTGGCGTTCGAAACCATg                                                                            >  2:542121/1‑140 (MQ=255)
                                                                  gagaCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTc                                                                    >  1:225631/1‑140 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTc                                                                         <  2:789938/132‑1 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTc                                                                         >  1:789938/1‑132 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 <  1:178286/140‑1 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 <  2:273852/140‑1 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 >  2:313325/1‑140 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 >  2:1218681/1‑140 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 >  1:273852/1‑140 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 >  2:708955/1‑140 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 >  2:712607/1‑140 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCgg                                                                 >  1:721402/1‑140 (MQ=255)
                                                                     aCCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGACGGTGAAAGGGGGGGTTCAACTGGCGATCGAAACCATGGTCGATTCCgg                                                                 >  2:579808/1‑140 (MQ=255)
                                                                       cGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGca                                                               >  1:959158/1‑140 (MQ=255)
                                                                       cGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGca                                                               <  1:339919/140‑1 (MQ=255)
                                                                       cGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGca                                                               <  2:1131624/140‑1 (MQ=255)
                                                                       cGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGca                                                               <  2:959158/140‑1 (MQ=255)
                                                                       cGGCAAAACCGCGTTTGAAACCCCGCCGCAGTATGCAGGGAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGca                                                               <  2:490609/140‑1 (MQ=255)
                                                                          cAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGcatca                                                            >  1:289150/1‑140 (MQ=255)
                                                                          cAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGcatca                                                            >  2:753603/1‑140 (MQ=255)
                                                                          cAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGcatca                                                            <  2:875681/140‑1 (MQ=255)
                                                                          cAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTAGAAACCATGGTCGATTCCGGcatca                                                            >  1:490609/1‑140 (MQ=255)
                                                                              aCCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGa                                                        <  2:289150/140‑1 (MQ=255)
                                                                               ccGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGaa                                                       <  1:100461/140‑1 (MQ=255)
                                                                               ccGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGaa                                                       <  1:973446/140‑1 (MQ=255)
                                                                                  cgTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAagag                                                    <  1:1257560/140‑1 (MQ=255)
                                                                                   gTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGt                                                   <  1:110536/140‑1 (MQ=255)
                                                                                    tttGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTc                                                  <  1:1218681/140‑1 (MQ=255)
                                                                                      tGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTg                                                <  1:990283/140‑1 (MQ=255)
                                                                                        aaaCCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCa                                              <  2:15468/140‑1 (MQ=255)
                                                                                        aaaCCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCa                                              <  1:753603/140‑1 (MQ=255)
                                                                                        aaaCCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCa                                              <  1:922424/140‑1 (MQ=255)
                                                                                        aaaCCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCa                                              <  2:846121/140‑1 (MQ=255)
                                                                                               gccgcAGTATGAAGGCAAAATCCGCCAGCCGGAGTACCTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATg                                       <  1:397893/140‑1 (MQ=255)
                                                                                                ccgcAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGa                                      >  1:66225/1‑140 (MQ=255)
                                                                                                   cAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCa                                              <  2:756002/129‑1 (MQ=255)
                                                                                                   cAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCa                                              >  1:756002/1‑129 (MQ=255)
                                                                                                        tGAAGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGc                              >  2:51337/1‑140 (MQ=255)
                                                                                                           aGGCAAAATCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACg                           >  1:1321911/1‑140 (MQ=255)
                                                                                                                aaaTCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTg                      >  2:826684/1‑140 (MQ=255)
                                                                                                                aaaTCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTg                      >  2:253653/1‑140 (MQ=255)
                                                                                                                aaaTCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTg                      >  1:838842/1‑140 (MQ=255)
                                                                                                                aaaTCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTg                      >  1:1375298/1‑140 (MQ=255)
                                                                                                                 aaTCGGCGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGc                     <  2:1321911/140‑1 (MQ=255)
                                                                                                                       cGAGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGa               >  2:289861/1‑140 (MQ=255)
                                                                                                                       agacagggAGTACTTCTTTAAACGCGTACTGCTGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGa               <  2:494188/134‑1 (MQ=255)
                                                                                                                         aGCAGGAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGAtt             <  1:51337/140‑1 (MQ=255)
                                                                                                                             ggAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCa         <  2:838842/140‑1 (MQ=255)
                                                                                                                             ggAGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCa         <  2:1375298/140‑1 (MQ=255)
                                                                                                                               aGTACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCAac       <  1:826684/140‑1 (MQ=255)
                                                                                                                                 tACTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCAacac     <  1:1081399/140‑1 (MQ=255)
                                                                                                                                   cTTCTATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCAACACCa   <  1:708955/140‑1 (MQ=255)
                                                                                                                                    ttCTATAAAGGCGTACTGATGATTGGGATTGTGAAAGTGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCAACACCAt  <  1:542121/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GAATTCTCTTCCGGTATGATGGCGGACTGGGCCAACGATGATAAGAAACTGCTGACCTGGCGTGAAGAGACCGGCAAAACCGCGTTTGAAACCGCGCCGCAGTATGAAGGCAAAATCGGCGAGCAGGAGTACTTCGATAAAGGCGTACTGATGATTGCGATGGTGAAAGCGGGCGTTGAACTGGCGTTCGAAACCATGGTCGATTCCGGCATCATTGAAGAGTCTGCATATTATGAATCACTGCACGAGCTGCCGCTGATTGCCAACACCAT  >  NC_000913/3958900‑3959171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A