Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F60 I1 R1	17	198.2	6851676	99.0%	6783159	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,174,965	G→T	A21E (GCA→GAA)	coaA ←	pantothenate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,174,965	0	G	T	99.7%	1077.2 / NA	306	A21E (GCA→GAA)	coaA	pantothenate kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (168/137); minor base A (0/1); total (168/138)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

ATATAGAAGTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTGCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATG  >  NC_000913/4174830‑4175100

atataGAAGTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCa                                                                                                                                     >  2:93559/1‑140 (MQ=255)

atataGAAGTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCa                                                                                                                                     <  1:2132467/140‑1 (MQ=255)

     gAAGTTCAGCAAACGTGACAAATGTAAATAGATCTAGGCAACTTCTTCCAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGt                                                                                                                                <  2:1256661/140‑1 (MQ=255)

      aaGTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGtt                                                                                                                               >  1:465811/1‑140 (MQ=255)

      aaGTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGtt                                                                                                                               >  1:2464634/1‑140 (MQ=255)

      aaGTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGtt                                                                                                                               >  1:290929/1‑140 (MQ=255)

        gTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGc                                                                                                                             <  1:39968/140‑1 (MQ=255)

        gTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGc                                                                                                                             >  2:2135233/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCTTAGATTCTTAATCACGCAGAGCTTCCCACTCGTTGCGGt                                                                                                                          >  2:1664668/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCTCACTGGTTGCGGt                                                                                                                          >  1:2808295/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCa                                                                                                                                     >  1:3057255/1‑129 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  1:2877808/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  1:800225/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          <  2:3148088/140‑1 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  1:3364108/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  2:1687437/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  2:2187425/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  1:622291/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  1:1778722/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGt                                                                                                                          >  2:406367/1‑140 (MQ=255)

           cAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCCATAACGTCCTAGTTACGGAATCACGCAGAGATTCCCACTGGTTGCGGt                                                                                                                          >  2:1441380/1‑140 (MQ=255)

            aGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTc                                                                                                                         <  1:2988458/140‑1 (MQ=255)

             gCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCa                                                                                                                        <  2:3364108/140‑1 (MQ=255)

             gCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCa                                                                                                                        <  2:715256/140‑1 (MQ=255)

               aaaCGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCaaa                                                                                                                      <  2:1884917/140‑1 (MQ=255)

                aaCGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAAc                                                                                                                     <  1:554217/140‑1 (MQ=255)

                   gTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGt                                                                                                                  >  2:2880729/1‑140 (MQ=255)

                       cAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGt                                                                                                              <  2:2816887/140‑1 (MQ=255)

                        aaaGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTa                                                                                                             >  2:814432/1‑140 (MQ=255)

                          aGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAg                                                                                                           <  1:2019115/140‑1 (MQ=255)

                           ggTAAATAGATCTCGGCAACTCCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAgg                                                                                                          >  2:23560/1‑140 (MQ=255)

                               aaTAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTc                                                                                                      >  1:431974/1‑140 (MQ=255)

                                  aGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCAtt                                                                                                   <  1:1848773/140‑1 (MQ=255)

                                  aGATCTCGGCAACTTCTGCTAATGAGAGATCGTCAATAAGACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCAtt                                                                                                   <  2:1107557/140‑1 (MQ=255)

                                    aTCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTACGTAAGGCGTCATTaa                                                                                                 >  1:1565392/1‑140 (MQ=255)

                                      ctcGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCAGTGGTTGCGGTCTAACTGTAGGCAAGGCGTCATTAACg                                                                                               >  1:1527741/1‑140 (MQ=255)

                                      ctcGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACg                                                                                               >  2:149552/1‑140 (MQ=255)

                                      ctcGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACg                                                                                               >  2:1300395/1‑140 (MQ=255)

                                      ctcGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACg                                                                                               >  2:609301/1‑140 (MQ=255)

                                      ctcGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACg                                                                                               >  1:2387830/1‑140 (MQ=255)

                                      ctcGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACg                                                                                               >  2:3279273/1‑140 (MQ=255)

                                      ctcGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTAACACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGTCGTCATTacct                                                                                               >  2:2522993/1‑137 (MQ=255)

                                         ggCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTGAAAATGGAGGTAAAGCGTCATTAACGttt                                                                                            >  1:1252489/1‑140 (MQ=255)

                                         ggCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGATAAGGCGTCATTAACGttt                                                                                            >  1:3251704/1‑140 (MQ=255)

                                         ggCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGATTGCGGTGAAATTGGAGGTAAAGCGTCATTAACgctg                                                                                            >  1:1002841/1‑137 (MQ=255)

                                         ggCAACTTCTTCTAACGAGAGAACTACATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGttt                                                                                            <  1:1446815/140‑1 (MQ=255)

                                          gCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTg                                                                                           <  1:367896/140‑1 (MQ=255)

                                          gCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTg                                                                                           <  2:1778722/140‑1 (MQ=255)

                                          gCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTg                                                                                           <  1:2187425/140‑1 (MQ=255)

                                           cAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGc                                                                                          <  2:3071950/140‑1 (MQ=255)

                                            aaCTTCTTCTAACGAGACATCTCCATTAATAGCTTCGAGCCGGGCGATGTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGct                                                                                         <  2:1352765/140‑1 (MQ=255)

                                            aaCTTCTTATAACGAGTCATCTTCATTAATACCTTGGAGGCGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGct                                                                                         <  2:1565392/140‑1 (MQ=255)

                                              cttcttCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCg                                                                                                        <  2:633482/123‑1 (MQ=255)

                                                   tctAACGAGAGATCTTAGTTAATACCTTTGATAAGCGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTtat                                                                                  <  2:1527741/140‑1 (MQ=255)

                                                    ctAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTtata                                                                                 <  1:847275/140‑1 (MQ=255)

                                                              aTCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCAt                                                                            >  2:577129/1‑135 (MQ=255)

                                                                   cATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGttt                                                                  >  1:2075124/1‑140 (MQ=255)

                                                                      tAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTg                                                               <  1:149552/140‑1 (MQ=255)

                                                                       aaTACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTgg                                                              <  1:3275930/140‑1 (MQ=255)

                                                                       aaTACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTgg                                                              <  2:2626479/140‑1 (MQ=255)

                                                                        aTACCTTTGAGACGGGCGATCTCATTTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGc                                                             <  1:1092060/140‑1 (MQ=255)

                                                                        aTACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTgg                                                              >  1:2585713/1‑139 (MQ=255)

                                                                        aTACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTgg                                                              <  2:2585713/139‑1 (MQ=255)

                                                                        aTACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGc                                                             >  2:2025097/1‑140 (MQ=255)

                                                                         tACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAGCGTTTGCACTTATAGACTCATGAGCATGTTTCTGGCg                                                            >  1:996404/1‑140 (MQ=255)

                                                                         tACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCAt                                                                      >  1:2540530/1‑130 (MQ=255)

                                                                           ccTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGt                                                          >  1:23138/1‑140 (MQ=255)

                                                                           ccTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACATTTGCTCCTTTTTACTCATAATCATGTTTCTGGCGGt                                                          >  1:1675996/1‑140 (MQ=255)

                                                                              ttGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGt                                                          >  1:577297/1‑137 (MQ=255)

                                                                              ttGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCAt                                                       >  1:3417164/1‑140 (MQ=255)

                                                                               tGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATa                                                      >  1:697581/1‑140 (MQ=255)

                                                                                gagaCGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAg                                                     >  1:1994361/1‑140 (MQ=255)

                                                                                gagaCGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAg                                                     >  2:482983/1‑140 (MQ=255)

                                                                                      ggCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGGATGTTTCTGGCGGTCATAGCTCTAt                                               >  1:1339028/1‑140 (MQ=255)

                                                                                      ggCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTCATACTCAAAAGTATATTTCTGGCGGTCATAGCTCTAt                                               >  1:1281345/1‑140 (MQ=255)

                                                                                                 cTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAg                                       >  1:2659850/1‑137 (MQ=255)

                                                                                                  ttCCGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGat                                   <  2:3417164/140‑1 (MQ=255)

                                                                                                    gcGATAACGTCATAGATACGGAGTCCCGCCGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGatat                                 <  1:1121331/139‑1 (MQ=255)

                                                                                                    ccGATAACGTCATAGCTACGGACTCGCGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGatat                                 <  1:1624818/140‑1 (MQ=255)

                                                                                                     cGATAACGTCATAGGTACGTAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAACGataat                                >  2:2258650/1‑138 (MQ=255)

                                                                                                     cGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATAtt                                >  2:1983413/1‑140 (MQ=255)

                                                                                                     cGATAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATAtt                                >  2:740260/1‑140 (MQ=255)

                                                                                                       aTAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTGAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTGTCTGGCGTTCATAGATCTATCCGCAAAGCGATATTTg                              >  1:1387770/1‑140 (MQ=255)

                                                                                                       aTAACGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTGTCTGGCGGTCAGAGGTCTATCGGCAAAGCGATATTTg                              >  1:936220/1‑140 (MQ=255)

                                                                                                         aaCGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGcc                            >  1:2850749/1‑140 (MQ=255)

                                                                                                           cGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGTGATATTTGCCAt                          >  2:384360/1‑140 (MQ=255)

                                                                                                           cGTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCAt                          >  2:3019984/1‑140 (MQ=255)

                                                                                                            gTCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTACCGTTTGCTCTCTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATg                         <  2:2857019/140‑1 (MQ=255)

                                                                                                             tCATAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATgg                        <  2:768693/140‑1 (MQ=255)

                                                                                                               aTAGGTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGgcg                      <  2:2974515/140‑1 (MQ=255)

                                                                                                                  ggTACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGt                   <  2:1734769/140‑1 (MQ=255)

                                                                                                                    tACGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTca                 <  1:749139/140‑1 (MQ=255)

                                                                                                                      cGGAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTcaca               >  2:2779851/1‑140 (MQ=255)

                                                                                                                       ggAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTGGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAACTCAATCAGCAAAGCGATATTAGCCATGGCGCGTCACAg              >  2:1058145/1‑140 (MQ=255)

                                                                                                                       ggAATCACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAg              <  2:2677265/140‑1 (MQ=255)

                                                                                                                            cACGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTg         <  2:2470098/140‑1 (MQ=255)

                                                                                                                              cGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGtt       >  1:1907625/1‑140 (MQ=255)

                                                                                                                              cGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGtt       >  1:1714938/1‑140 (MQ=255)

                                                                                                                              cGCAGAGCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGtt       >  1:90132/1‑140 (MQ=255)

                                                                                                                                   agCTTCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTGTATCGGCACAGCGATATTTGCCATGGCGCGGCACAGTTCTGTTGAATg  >  2:1215898/1‑140 (MQ=255)

ATATAGAAGTTCAGCAAACGTGACAAAGGTAAATAGATCTCGGCAACTTCTTCTAACGAGAGATCTTCATTAATACCTTTGAGACGGGCGATCTCATCTTCCGATAACGTCATAGGTACGGAATCACGCAGAGCTGCCCACTGGTTGCGGTCAAACTGTAGGTAAGGCGTCATTAACGTTTGCTCTTTTATACTCATAAGCATGTTTCTGGCGGTCATAGCTCTATCGGCAAAGCGATATTTGCCATGGCGCGTCACAGTTCTGTTGAATG  >  NC_000913/4174830‑4175100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A