Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A46 F60 I1 R1	13	84.7	2877034	99.3%	2856894	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,062,315	A→C	L23F (TTA→TTC)	yihN →	putative transporter YihN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,062,315	0	A	C	99.2%	457.0 / ‑5.1	134	L23F (TTA→TTC)	yihN	putative transporter YihN
Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (76/57); minor base T (1/0); total (77/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 137 total aligned reads displayed.

TTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGC  >  NC_000913/4062179‑4062446

ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCg                                                                                                                                  <  1:834010/140‑1 (MQ=255)

ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCg                                                                                                                                  >  2:43688/1‑140 (MQ=255)

ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCg                                                                                                                                  >  2:912021/1‑140 (MQ=255)

ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCg                                                                                                                                  >  1:389057/1‑140 (MQ=255)

ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCg                                                                                                                                  <  2:329005/140‑1 (MQ=255)

ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCg                                                                                                                                  <  2:389057/140‑1 (MQ=255)

 tCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGt                                                                                                                                 >  2:524746/1‑140 (MQ=255)

 tCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGt                                                                                                                                 >  2:1421047/1‑140 (MQ=255)

  ccATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTc                                                                                                                                >  2:1061302/1‑140 (MQ=255)

            ggTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGAt                                                                                                                      <  1:43688/140‑1 (MQ=255)

            ggTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGAt                                                                                                                      <  1:140915/140‑1 (MQ=255)

             gTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCg                                                                                                                               >  2:1329770/1‑130 (MQ=255)

             gTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCg                                                                                                                               >  2:739924/1‑130 (MQ=255)

             gTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATg                                                                                                                     >  1:591313/1‑140 (MQ=255)

             gTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATg                                                                                                                     >  2:1358161/1‑140 (MQ=255)

              tCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGc                                                                                                                    <  1:299152/140‑1 (MQ=255)

              tCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGc                                                                                                                    <  1:1170455/140‑1 (MQ=255)

              tCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGc                                                                                                                    >  1:1336526/1‑140 (MQ=255)

                    tCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTta                                                                                                              <  1:1317703/140‑1 (MQ=255)

                       tATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTatat                                                                                                           >  1:1186351/1‑140 (MQ=255)

                        aTTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTatatt                                                                                                          >  1:1415963/1‑139 (MQ=255)

                        aTTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATc                                                                                                          >  1:1241551/1‑140 (MQ=255)

                        aTTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATc                                                                                                          >  1:615658/1‑140 (MQ=255)

                         ttGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATcc                                                                                                         >  2:32980/1‑140 (MQ=255)

                            ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGAt                                                                                                                      >  1:218565/1‑124 (MQ=255)

                            ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGtt                                                                                                                 >  1:315181/1‑129 (MQ=255)

                            ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGa                                                                                                      >  1:1050807/1‑140 (MQ=255)

                            ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGa                                                                                                      >  1:871161/1‑140 (MQ=255)

                            ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGa                                                                                                      >  2:618823/1‑140 (MQ=255)

                            ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGa                                                                                                      >  2:1064107/1‑140 (MQ=255)

                            ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGa                                                                                                      <  2:871161/140‑1 (MQ=255)

                              tttAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATg                                                                                                    >  1:646858/1‑140 (MQ=255)

                              tttAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATg                                                                                                    >  1:1207290/1‑140 (MQ=255)

                                tAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCa                                                                                                  >  2:480514/1‑140 (MQ=255)

                                   aaCAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGa                                                                                               <  1:1064107/140‑1 (MQ=255)

                                      aGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAata                                                                                            >  1:1155954/1‑140 (MQ=255)

                                       gTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATg                                                                                                    <  2:1262015/131‑1 (MQ=255)

                                       gTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATg                                                                                                    >  1:1262015/1‑131 (MQ=255)

                                       gTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCa                                                                                                  >  2:369385/1‑133 (MQ=255)

                                       gTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCa                                                                                                  <  1:369385/133‑1 (MQ=255)

                                        tGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATAtt                                                                                          >  2:824093/1‑140 (MQ=255)

                                        tGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATAtt                                                                                          <  1:915522/140‑1 (MQ=255)

                                        tGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATAtt                                                                                          <  1:184170/140‑1 (MQ=255)

                                             ttAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCAt                                                                                     <  1:472240/140‑1 (MQ=255)

                                               aaaaaTCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCAttt                                                                                   <  1:187374/140‑1 (MQ=255)

                                                  aaTCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTc                                                                                        >  2:306086/1‑132 (MQ=255)

                                                              gATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAAt                                                                    <  1:824093/140‑1 (MQ=255)

                                                                 gTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTgg                                                                 <  2:1207290/140‑1 (MQ=255)

                                                                 gTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTgg                                                                 >  1:357505/1‑140 (MQ=255)

                                                                 gTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTgg                                                                 <  2:62702/140‑1 (MQ=255)

                                                                  ttATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCCCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGt                                                                >  1:1328089/1‑140 (MQ=255)

                                                                      gCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTaa                                                              <  1:925506/138‑1 (MQ=255)

                                                                      gCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTaa                                                              >  2:925506/1‑138 (MQ=255)

                                                                      gCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATg                                                            >  2:315542/1‑140 (MQ=255)

                                                                      gCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGGCAAATTGGTAATg                                                            >  2:1299213/1‑140 (MQ=255)

                                                                       cTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGc                                                           >  1:1239008/1‑140 (MQ=255)

                                                                         cACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTa                                                         <  2:422748/140‑1 (MQ=255)

                                                                          aCGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGt                                                                         <  1:931466/123‑1 (MQ=255)

                                                                          aCGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGt                                                                         >  2:931466/1‑123 (MQ=255)

                                                                          aCGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCAATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTAt                                                        >  2:286485/1‑140 (MQ=255)

                                                                            gAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTaa                                                              <  1:1172261/132‑1 (MQ=255)

                                                                            gAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTaa                                                              >  2:1172261/1‑132 (MQ=255)

                                                                                       gggCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCa                                           <  1:794828/140‑1 (MQ=255)

                                                                                         gCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAtt                                         <  1:873274/140‑1 (MQ=255)

                                                                                          cGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAttt                                        >  1:240168/1‑140 (MQ=255)

                                                                                                 tAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTcacca                                 <  1:32980/140‑1 (MQ=255)

                                                                                                   gTCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACcaca                               <  2:240168/140‑1 (MQ=255)

                                                                                                    tCTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAg                              >  2:1142228/1‑140 (MQ=255)

                                                                                                     cTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGt                             <  1:325285/140‑1 (MQ=255)

                                                                                                     cTATTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGt                             <  2:1014158/140‑1 (MQ=255)

                                                                                                       aTTAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTgg                           >  1:451642/1‑140 (MQ=255)

                                                                                                        ttAACACTGTGTGGCGGTACAATTTATAAATTCCCCTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTggg                          >  1:140990/1‑140 (MQ=255)

                                                                                                         tAACACTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGc                         >  2:1226093/1‑140 (MQ=255)

                                                                                                              cTGTGTGGCGGTACAATTTATAAATTTCCGTCGCTGAAAGATGCGTTTTTTATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAAATCATTTGTCACCACAGTGGGCttttt                    >  2:586056/1‑140 (MQ=255)

                                                                                                              cTGTGTGGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCttttt                    >  1:637792/1‑140 (MQ=255)

                                                                                                               tgtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCtttttt                   <  1:1013220/140‑1 (MQ=255)

                                                                                                                gtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGc                         >  2:1181879/1‑133 (MQ=255)

                                                                                                                gtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCttttttt                  >  1:584354/1‑139 (MQ=255)

                                                                                                                gtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCttttttt                  >  2:50535/1‑139 (MQ=255)

                                                                                                                gtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCttttttt                  >  2:140550/1‑139 (MQ=255)

                                                                                                                gtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCtttttt                   <  1:1127425/139‑1 (MQ=255)

                                                                                                                gtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCtttttt                   >  2:1127425/1‑139 (MQ=255)

                                                                                                                gtgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTc                  >  1:1054336/1‑140 (MQ=255)

                                                                                                                 tgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCt                 >  1:1299893/1‑140 (MQ=255)

                                                                                                                 tgtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCt                 >  1:1342895/1‑140 (MQ=255)

                                                                                                                  gtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTg                >  1:1285076/1‑140 (MQ=255)

                                                                                                                  gtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTg                >  1:1238196/1‑140 (MQ=255)

                                                                                                                  gtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTg                >  1:579415/1‑140 (MQ=255)

                                                                                                                  gtgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTg                <  1:1358161/140‑1 (MQ=255)

                                                                                                                   tgGCGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGGAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGt               >  2:203522/1‑140 (MQ=255)

                                                                                                                      cGGTACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCta            >  1:1080317/1‑140 (MQ=255)

                                                                                                                         tACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCtattt         >  2:971861/1‑140 (MQ=255)

                                                                                                                         tACAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCtattt         >  2:89036/1‑140 (MQ=255)

                                                                                                                         caCAATTTATAAATTCCCGCAGCTGAAAGAGGCGTTTTATATCCCGATGCAGGAATAATTTCACTTGACCAATGGCCAAATTGGTAATGCTATGTCGGGAAACTCATTTGTCACCACAGGGGGCCTTTTTCTGACtattt         >  2:859947/2‑140 (MQ=255)

                                                                                                                           cAATTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCtatttat       >  2:864330/1‑140 (MQ=255)

                                                                                                                            aaTTTATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCtatttatt      <  1:524746/140‑1 (MQ=255)

                                                                                                                              tttATAAATTCCCGTCGCTGGAAGAAGCGCTTTTTATCCCGATGCAGGAATATTTCCATTTGACCAATGGTAAAATTGGTAATGCTATGTTCGCAAACTCATTTTTCATCACAGAGGGCTTTTTTTTGTTTAtttttttt    >  2:451357/1‑140 (MQ=255)

                                                                                                                              tttATAAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTAtttt    <  1:99495/140‑1 (MQ=255)

                                                                                                                                tataAATTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGc  <  1:89036/140‑1 (MQ=255)

                                                                                                                                   aaaTTCCCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCtatttatt      >  2:1212807/1‑133 (MQ=255)

TTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGC  >  NC_000913/4062179‑4062446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A