Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	124	90.1	3003320	99.2%	2979293	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,334,450	T→C	T381A (ACC→GCC)	eptA ←	phosphoethanolamine transferase EptA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,334,450	0	T	C	100.0%	440.4 / NA	115	T381A (ACC→GCC)	eptA	phosphoethanolamine transferase EptA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (54/61); total (54/61)

Only 100 of 118 total aligned reads displayed.

AGCGTGTTGTCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGTGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAGGCACG  >  NC_000913/4334317‑4334586

aGCGTGTTGTCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTaa                                                                                                                                    <  1:777917/140‑1 (MQ=255)

  cGTGTTGTCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGa                                                                                                                                  >  1:325026/1‑140 (MQ=255)

  cGTGTTGTCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGa                                                                                                                                  >  2:763125/1‑140 (MQ=255)

   gtgtTGTCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGAc                                                                                                                                 >  2:132662/1‑140 (MQ=255)

      ttgtCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAAt                                                                                                                              >  1:1055604/1‑140 (MQ=255)

      ttgtCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAAt                                                                                                                              <  1:1493755/140‑1 (MQ=255)

      ttgtCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAAt                                                                                                                              <  2:1337913/140‑1 (MQ=255)

           gTAAGTGTTCACCAGTTGCTCTTTGGTTCAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGc                                                                                                                         <  1:707545/140‑1 (MQ=255)

              aGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCAt                                                                                                                      <  1:710402/140‑1 (MQ=255)

                tgtTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCa                                                                                                                    <  1:42528/140‑1 (MQ=255)

                tgtTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCa                                                                                                                    <  1:1465155/140‑1 (MQ=255)

                  ttCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCAcc                                                                                                                  >  2:963015/1‑140 (MQ=255)

                   tCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCt                                                                                                                 >  2:677823/1‑140 (MQ=255)

                   tCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCt                                                                                                                 >  2:456105/1‑140 (MQ=255)

                   tCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCt                                                                                                                 >  1:905151/1‑140 (MQ=255)

                     cccAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTg                                                                                                               <  2:791972/139‑1 (MQ=255)

                     aCCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTg                                                                                                               <  2:388779/140‑1 (MQ=255)

                     aCCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTg                                                                                                               <  2:251849/140‑1 (MQ=255)

                     aCCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTg                                                                                                               <  2:1050970/140‑1 (MQ=255)

                      ccAGTTGCTCTTTGGTTCAGGTCTGGATCTCATTGGTGTCGCAGGTTGGTGTAAATTTCCTTAACTGGGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGc                                                                                                              <  2:1472631/140‑1 (MQ=255)

                          ttGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGt                                                                                                          <  1:68630/140‑1 (MQ=255)

                          ttGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGt                                                                                                          <  2:500659/140‑1 (MQ=255)

                          ttGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGt                                                                                                          >  2:95574/1‑140 (MQ=255)

                            gCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTa                                                                                                        >  2:86507/1‑140 (MQ=255)

                            gCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTa                                                                                                        >  1:568962/1‑140 (MQ=255)

                                 ttGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGAt                                                                                                   >  2:1255135/1‑140 (MQ=255)

                                  tGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATg                                                                                                  <  2:1435403/140‑1 (MQ=255)

                                    gTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTa                                                                                                <  2:1288589/140‑1 (MQ=255)

                                    gTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTa                                                                                                <  2:139086/140‑1 (MQ=255)

                                    gTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTa                                                                                                <  2:325026/140‑1 (MQ=255)

                                    gTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTa                                                                                                <  1:739278/140‑1 (MQ=255)

                                    gTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTa                                                                                                <  2:932536/140‑1 (MQ=255)

                                    gTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTa                                                                                                <  2:115031/140‑1 (MQ=255)

                                    gTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTa                                                                                                <  1:1454914/140‑1 (MQ=255)

                                          gTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTTTTGATGGACTCTTc                                                                                          >  2:540062/1‑140 (MQ=255)

                                            cTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCaa                                                                                        >  2:213291/1‑140 (MQ=255)

                                               gATCTCGTTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGcc                                                                                     >  1:1134140/1‑140 (MQ=255)

                                               gATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGcc                                                                                     >  2:295838/1‑140 (MQ=255)

                                                 tctcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCg                                                                                   <  1:584375/140‑1 (MQ=255)

                                                 tctcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCg                                                                                   <  1:1009749/140‑1 (MQ=255)

                                                 tctcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCg                                                                                   <  1:1065852/140‑1 (MQ=255)

                                                 tctcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCg                                                                                   <  1:277081/140‑1 (MQ=255)

                                                 tctcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCg                                                                                   <  2:1055604/140‑1 (MQ=255)

                                                 tctcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCg                                                                                   <  2:1352111/140‑1 (MQ=255)

                                                 tctcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCg                                                                                   <  1:1486693/140‑1 (MQ=255)

                                                   tcATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTg                                                                                 <  2:356205/140‑1 (MQ=255)

                                                    cATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTgg                                                                                >  1:908651/1‑140 (MQ=255)

                                                    cATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTgg                                                                                >  2:809804/1‑140 (MQ=255)

                                                    cATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTgg                                                                                >  2:135419/1‑140 (MQ=255)

                                                    cATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTgg                                                                                >  2:317715/1‑140 (MQ=255)

                                                    cATTGGTGTCGCAGGGTGGGGGAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTCTTGATGTACTCTTCAAGCCCGTgg                                                                                >  2:1160882/1‑140 (MQ=255)

                                                    cATTGGTGACGCAGGTTGGGGGAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGGTGCCGATGGCGTGATAGACAATCACGCCATCACCATGCAGGTAATTGATGCACTCTTCAAGCCCGTgg                                                                                >  2:679689/1‑140 (MQ=255)

                                                      ttGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGaa                                                                              <  1:40326/140‑1 (MQ=255)

                                                         gtgtCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGGCCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAg                                                                           <  2:290433/140‑1 (MQ=255)

                                                         gtgtCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAg                                                                           >  1:1058976/1‑140 (MQ=255)

                                                         gtgtCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAg                                                                           <  1:1335393/140‑1 (MQ=255)

                                                            tCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCAc                                                                        <  1:665514/140‑1 (MQ=255)

                                                            tCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCAc                                                                        <  1:1476563/140‑1 (MQ=255)

                                                            tCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCAc                                                                        >  1:1181427/1‑140 (MQ=255)

                                                            tCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCAc                                                                        >  2:679474/1‑140 (MQ=255)

                                                             cGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACt                                                                       <  1:1083772/140‑1 (MQ=255)

                                                             cGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACt                                                                       <  2:224561/140‑1 (MQ=255)

                                                               cAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTc                                                                     >  2:472332/1‑140 (MQ=255)

                                                                     ggggTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATa                                                               >  1:227612/1‑140 (MQ=255)

                                                                            aTTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCg                                                        >  2:852782/1‑140 (MQ=255)

                                                                             tttCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGc                                                       <  2:1172395/140‑1 (MQ=255)

                                                                             tttCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGc                                                       <  1:38805/140‑1 (MQ=255)

                                                                                  tGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTg                                                  <  2:154945/140‑1 (MQ=255)

                                                                                  tGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTg                                                  <  2:374141/140‑1 (MQ=255)

                                                                                     aCTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATg                                               <  1:132662/140‑1 (MQ=255)

                                                                                     aCTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATg                                               >  1:1256528/1‑140 (MQ=255)

                                                                                      cTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGc                                              <  2:573223/140‑1 (MQ=255)

                                                                                        gAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCAc                                            <  2:926345/140‑1 (MQ=255)

                                                                                        gAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCACCGTTGATGCAc                                            <  1:963015/140‑1 (MQ=255)

                                                                                            cGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGAt                                        <  1:677823/140‑1 (MQ=255)

                                                                                            cGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGAt                                        <  1:312385/140‑1 (MQ=255)

                                                                                            cGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATCCGCCGTTGATGCACTGAt                                        <  1:1379768/140‑1 (MQ=255)

                                                                                              gATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCa                                      >  1:1221015/1‑140 (MQ=255)

                                                                                                tAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAgg                                    >  1:866768/1‑140 (MQ=255)

                                                                                                   cGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAg                                 >  2:300358/1‑140 (MQ=255)

                                                                                                   cGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAg                                 >  2:965280/1‑140 (MQ=255)

                                                                                                   cGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAg                                 >  1:552291/1‑140 (MQ=255)

                                                                                                        gTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCa                            >  2:625716/1‑140 (MQ=255)

                                                                                                        gTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCa                            >  1:173869/1‑140 (MQ=255)

                                                                                                        gTAATAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCa                            >  2:1278344/1‑140 (MQ=255)

                                                                                                           aTAGGTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAgcg                         <  1:1255135/140‑1 (MQ=255)

                                                                                                              ggTCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCgg                      >  1:922501/1‑140 (MQ=255)

                                                                                                                tCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTg                    >  2:1023064/1‑140 (MQ=255)

                                                                                                                tCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTg                    >  1:1074676/1‑140 (MQ=255)

                                                                                                                tCGGACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTg                    >  2:671153/1‑140 (MQ=255)

                                                                                                                   gACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTGCTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACg                 <  2:1092836/140‑1 (MQ=255)

                                                                                                                   gACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACg                 >  2:478559/1‑140 (MQ=255)

                                                                                                                   gACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACg                 >  2:297666/1‑140 (MQ=255)

                                                                                                                   gACCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACg                 >  1:132141/1‑140 (MQ=255)

                                                                                                                    aCCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGt                >  2:1300045/1‑140 (MQ=255)

                                                                                                                    aCCGTGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGt                <  1:1286807/140‑1 (MQ=255)

                                                                                                                        tGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGTTCTg            >  2:812417/1‑140 (MQ=255)

                                                                                                                        tGGCTGCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGTTCTg            >  2:289222/1‑140 (MQ=255)

                                                                                                                             gCCGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAg       <  1:135419/140‑1 (MQ=255)

                                                                                                                              ccGATGGCGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAgg      >  1:920187/1‑140 (MQ=255)

AGCGTGTTGTCGTAAGTGTTCACCAGTTGCTCTTTGGTACAGGTCTGGATCTCATTGGTGTCGCAGGTTGGGGTAAATTTCCTGAACTGAGGCGGATAGCGGTTGTAATAGGTCGGACCGTGGCTGCCGATGGTGTGTAAGACAATCACGCCATCACCTTGCAGGTTATTGATGTACTCTTCAAGCCCGTGGAACAGCACTTCGTCATAGCATTCGCCGTTGATGCACTGATCAGGTAGATTCAGCGCGGTGACGTTCTGGTGAGGCACG  >  NC_000913/4334317‑4334586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A