Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,521,565	(A)_6→7	coding (456/1350 nt)	yjhF ←	KpLE2 phage‑like element; putative transporter YjhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,521,559	1	.	A	100.0%	190.1 / NA	56	L154F (TTG→TTT)	yjhF	KpLE2 phage‑like element; putative transporter YjhF
Reads supporting (aligned to +/- strand): ref base . (0/0); new base A (47/9); total (47/9)

CAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGC‑AAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCGA  >  NC_000913/4521431‑4521692

cAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGC‑aaaaaaaccgt                                                                                                                            <  1:772308/140‑6 (MQ=255)

   aaTTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGtgtg                                                                                                                          <  1:362659/140‑1 (MQ=255)

     tttATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCg                                                                                                                        >  1:974438/1‑140 (MQ=255)

         ttCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCa                                                                                                                    >  2:802235/1‑140 (MQ=255)

                   ggCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGa                                                                                                          >  2:531131/1‑140 (MQ=255)

                   ggCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGa                                                                                                          >  2:289892/1‑140 (MQ=255)

                      cGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAg                                                                                                       >  1:302238/1‑140 (MQ=255)

                        cAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCa                                                                                                     >  1:14928/1‑140 (MQ=255)

                        cAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCa                                                                                                     >  1:190851/1‑140 (MQ=255)

                            gAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCgg                                                                                                 <  1:484720/140‑1 (MQ=255)

                               tAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGcac                                                                                              >  2:452029/1‑140 (MQ=255)

                                aaaGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGcaca                                                                                             <  1:47202/140‑1 (MQ=255)

                                       ggAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATaa                                                                                      >  2:856808/1‑140 (MQ=255)

                                       ggAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATaa                                                                                      >  2:562043/1‑140 (MQ=255)

                                              cGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCgg                                                                               >  1:879141/1‑140 (MQ=255)

                                                aCAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTg                                                                             >  1:139722/1‑140 (MQ=255)

                                                          cGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGc                                                                   >  2:878675/1‑140 (MQ=255)

                                                            tataTCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCtt                                                                 >  2:899655/1‑140 (MQ=255)

                                                            tataTCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCtt                                                                 >  2:899656/1‑140 (MQ=255)

                                                               atCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCAc                                                              <  1:289892/140‑1 (MQ=255)

                                                                   gTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGc                                                          >  2:710248/1‑140 (MQ=255)

                                                                    tACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCa                                                         >  1:650049/1‑140 (MQ=255)

                                                                    tACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCa                                                         >  2:411685/1‑140 (MQ=255)

                                                                    tACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCa                                                         >  1:44501/1‑140 (MQ=255)

                                                                         aTCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGc                                                    >  2:168941/1‑140 (MQ=255)

                                                                         aTCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGc                                                    >  1:807998/1‑140 (MQ=255)

                                                                         aTCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGc                                                    >  2:487360/1‑140 (MQ=255)

                                                                           ccTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGa                                                  >  1:192427/1‑140 (MQ=255)

                                                                           ccTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGACAATAGCGa                                                  >  2:471866/1‑140 (MQ=255)

                                                                              aCATCAGCGCCATATTCCCTGGCGATAACAGTCGTGCCGGGGGGCGGCGGCAAAAAAAACGTGTGCGACCAGCAAACCAGAAAGCATCGGCGCACACATAAACATCGGCGATATTTTTGCTTCACGGGCAATAGCGAATa                                               >  2:26074/1‑140 (MQ=255)

                                                                              aCATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATa                                               <  1:802235/140‑1 (MQ=255)

                                                                                 tCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATaaaa                                            >  2:694530/1‑140 (MQ=255)

                                                                                  cAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAAt                                           >  2:554098/1‑140 (MQ=255)

                                                                                    gcgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAg                                         >  1:513081/1‑140 (MQ=255)

                                                                                    gcgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAg                                         >  1:116431/1‑140 (MQ=255)

                                                                                     cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:119269/1‑140 (MQ=255)

                                                                                     cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  2:91826/1‑140 (MQ=255)

                                                                                     cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:193995/1‑140 (MQ=255)

                                                                                     cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  2:228090/1‑140 (MQ=255)

                                                                                     cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:454312/1‑140 (MQ=255)

                                                                                     cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:757839/1‑140 (MQ=255)

                                                                                     cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:700443/1‑140 (MQ=255)

                                                                                       ccATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTa                                      >  1:440816/1‑140 (MQ=255)

                                                                                           atTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCag                                  <  2:757839/140‑1 (MQ=255)

                                                                                                  ggCGATAACAGTCTGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACAAATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  2:465461/1‑140 (MQ=255)

                                                                                                  ggCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  2:418016/1‑140 (MQ=255)

                                                                                                  ggCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  2:580427/1‑140 (MQ=255)

                                                                                                  ggCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  1:606035/1‑140 (MQ=255)

                                                                                                    cGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTaa                         >  1:170765/1‑140 (MQ=255)

                                                                                                    cGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAAGATTaa                         >  2:750424/1‑140 (MQ=255)

                                                                                                      aTAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAAc                       >  2:360340/1‑140 (MQ=255)

                                                                                                        aaCAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCg                     >  2:406541/1‑140 (MQ=255)

                                                                                                             tCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTc                >  1:394457/1‑140 (MQ=255)

                                                                                                             tCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGGACCAGAAGAATTAAACCGACTTc                >  1:172448/1‑140 (MQ=255)

                                                                                                               gggCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGa              >  1:847275/1‑140 (MQ=255)

                                                                                                               gggCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGa              >  1:985097/1‑140 (MQ=255)

                                                                                                                        tGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTg     <  2:974438/140‑1 (MQ=255)

                                                                                                                          cggcggCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCg   <  1:899655/140‑1 (MQ=255)

                                                                                                                          cggcggCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCg   <  1:899656/140‑1 (MQ=255)

                                                                                                                           ggcggcaAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCGa  >  2:45030/1‑140 (MQ=255)

CAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGC‑AAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCGA  >  NC_000913/4521431‑4521692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A