Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,391,437	A→G	S25P (TCT→CCT)	rsgA ←	ribosome small subunit‑dependent GTPase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,391,437	0	A	G	100.0%	387.9 / NA	104	S25P (TCT→CCT)	rsgA	ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (47/57); total (47/57)

Only 100 of 109 total aligned reads displayed.

ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGATCAA  >  NC_000913/4391301‑4391573

aTGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGt                                                                                                                                       >  2:1294920/1‑140 (MQ=255)

        gCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAgac                                                                                                                               >  2:771458/1‑140 (MQ=255)

         cGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAgacg                                                                                                                              <  2:1334739/140‑1 (MQ=255)

          gAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCGTCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAgacga                                                                                                                             <  2:646861/140‑1 (MQ=255)

          gAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAgacga                                                                                                                             <  2:829609/140‑1 (MQ=255)

              atTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCt                                                                                                                         >  2:28804/1‑140 (MQ=255)

              atTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCt                                                                                                                         >  2:616/1‑140 (MQ=255)

                   aGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTCTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGa                                                                                                                    >  2:1466871/1‑140 (MQ=255)

                       gTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTgg                                                                                                                >  1:486578/1‑140 (MQ=255)

                       gTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTgg                                                                                                                >  2:405086/1‑140 (MQ=255)

                       gTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTgg                                                                                                                >  1:598824/1‑140 (MQ=255)

                       gTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTgg                                                                                                                >  2:1420476/1‑140 (MQ=255)

                        tGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGc                                                                                                               >  1:155312/1‑140 (MQ=255)

                        cgAACGTCGCACTCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGc                                                                                                               <  2:695144/139‑1 (MQ=255)

                               cGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCAcg                                                                                                        >  2:1411682/1‑140 (MQ=255)

                               cGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAAGCCCGCCCAACAGATTGTCGTCGTAGACGGGCTTCTCCTTTAGCGTTTTAAAACGACGCCGGGGAATGGCGTTCAcg                                                                                                        >  2:952891/1‑140 (MQ=255)

                                 ccATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCAcgcg                                                                                                      >  2:1040953/1‑140 (MQ=255)

                                  cATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACgcgg                                                                                                     <  1:1264246/140‑1 (MQ=255)

                                  cATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACgcgg                                                                                                     <  1:170147/140‑1 (MQ=255)

                                  cATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACgcgg                                                                                                     <  2:1035026/140‑1 (MQ=255)

                                  cATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACgcgg                                                                                                     <  1:354134/140‑1 (MQ=255)

                                         ggATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCgctgct                                                                                              >  2:144848/1‑140 (MQ=255)

                                         ggATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCgctgct                                                                                              <  1:168131/140‑1 (MQ=255)

                                           aTTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTgg                                                                                            >  2:1236562/1‑140 (MQ=255)

                                                acaTCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCttt                                                                                       >  2:888932/1‑140 (MQ=255)

                                                 caTCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTg                                                                                      >  2:1434895/1‑140 (MQ=255)

                                                  aTCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTgg                                                                                     <  1:1236562/140‑1 (MQ=255)

                                                       cGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGt                                                                                >  2:271992/1‑140 (MQ=255)

                                                       cGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGt                                                                                >  2:1072225/1‑140 (MQ=255)

                                                       cGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGt                                                                                >  2:1487005/1‑140 (MQ=255)

                                                               ccAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTAttttt                                                                        >  1:462646/1‑140 (MQ=255)

                                                                cAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTa                                                                       >  1:255152/1‑140 (MQ=255)

                                                                cAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTa                                                                       <  1:376253/140‑1 (MQ=255)

                                                                cAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTa                                                                       <  1:571144/140‑1 (MQ=255)

                                                                  aaGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACt                                                                     <  1:426686/140‑1 (MQ=255)

                                                                    gCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCa                                                                   <  1:320720/140‑1 (MQ=255)

                                                                    gCGGCTGATGACGATACCTTCATCAGGCTCACCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCa                                                                   <  1:1299533/140‑1 (MQ=255)

                                                                     cGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCaa                                                                  >  1:468030/1‑140 (MQ=255)

                                                                        cTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCg                                                               >  2:498013/1‑140 (MQ=255)

                                                                        cTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCg                                                               >  2:336877/1‑140 (MQ=255)

                                                                        cTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCg                                                               >  1:1313195/1‑140 (MQ=255)

                                                                        cTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCg                                                               >  2:385100/1‑140 (MQ=255)

                                                                        cTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCg                                                               >  1:34897/1‑140 (MQ=255)

                                                                        cTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCg                                                               >  1:1197695/1‑140 (MQ=255)

                                                                          gatgaCGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTa                                                             >  1:335122/1‑140 (MQ=255)

                                                                            tgaCGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTGTTAAGACGACGCTGGGGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACa                                                           >  2:1270493/1‑140 (MQ=255)

                                                                             gaCGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAg                                                          <  2:1045135/140‑1 (MQ=255)

                                                                                 aTACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTc                                                      <  1:547745/140‑1 (MQ=255)

                                                                                  tACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTcc                                                     >  1:476626/1‑140 (MQ=255)

                                                                                    ccTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTg                                                   <  1:29779/140‑1 (MQ=255)

                                                                                     cTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGGGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTgg                                                  >  1:439435/1‑140 (MQ=255)

                                                                                     cTTCATCAGGCTCGCCAAACAGATTGTCGCCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGCCGCTGGCGATTGGCGTTCACGCGGCGCTGCAGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTgg                                                  >  2:1431410/1‑140 (MQ=255)

                                                                                      ttCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGt                                                 <  1:771458/140‑1 (MQ=255)

                                                                                      ttCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTACTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGt                                                 <  1:1044004/140‑1 (MQ=255)

                                                                                        catcaGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCg                                               >  1:78807/1‑140 (MQ=255)

                                                                                         atcaGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGc                                              >  1:402518/1‑140 (MQ=255)

                                                                                         atcaGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGc                                              >  2:1424866/1‑140 (MQ=255)

                                                                                              gCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTg                                         >  2:743424/1‑140 (MQ=255)

                                                                                              gCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTg                                         >  2:1466757/1‑140 (MQ=255)

                                                                                              gCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTg                                         >  1:118791/1‑140 (MQ=255)

                                                                                               cTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGTCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGa                                        <  1:1466871/140‑1 (MQ=255)

                                                                                               cTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGa                                        >  2:1043593/1‑140 (MQ=255)

                                                                                               cTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGa                                        >  1:831338/1‑140 (MQ=255)

                                                                                                   ccAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATggg                                    <  2:1267694/140‑1 (MQ=255)

                                                                                                      aaCAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCaa                                 <  1:405086/140‑1 (MQ=255)

                                                                                                      aaCAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCaa                                 <  2:486578/140‑1 (MQ=255)

                                                                                                       aCAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCaaa                                <  1:498013/140‑1 (MQ=255)

                                                                                                       aCAGATTGTCGGCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGGTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCaaa                                <  2:402518/140‑1 (MQ=255)

                                                                                                         aGATTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAAc                              <  1:144848/140‑1 (MQ=255)

                                                                                                           aTTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACAt                            <  1:952891/140‑1 (MQ=255)

                                                                                                           aTTGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACAt                            <  2:78807/140‑1 (MQ=255)

                                                                                                            ttGTCGTCGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATc                           >  1:995885/1‑140 (MQ=255)

                                                                                                              gtcgtcGTAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTa                         <  2:598824/140‑1 (MQ=255)

                                                                                                                   cgtAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATa                    <  2:468030/140‑1 (MQ=255)

                                                                                                                     tAGTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATaca                  <  1:1411682/140‑1 (MQ=255)

                                                                                                                       gTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACg                <  1:1067786/140‑1 (MQ=255)

                                                                                                                       gTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACg                <  1:1541044/140‑1 (MQ=255)

                                                                                                                       gTCGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACg                <  2:427009/140‑1 (MQ=255)

                                                                                                                         cGGGCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCa              >  2:485613/1‑140 (MQ=255)

                                                                                                                            gCTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAAtt           <  2:427324/140‑1 (MQ=255)

                                                                                                                             cTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATtg          <  1:1040953/140‑1 (MQ=255)

                                                                                                                             cTTCTCCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATtg          <  2:37900/140‑1 (MQ=255)

                                                                                                                               tctcCTTAGGCGTTTTAGGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATtgtg        <  2:157849/140‑1 (MQ=255)

                                                                                                                               tctcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATtgtg        <  1:1072225/140‑1 (MQ=255)

                                                                                                                               tctcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATtgtg        <  1:616/140‑1 (MQ=255)

                                                                                                                               tctcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATtgtg        <  1:743424/140‑1 (MQ=255)

                                                                                                                               tctcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATtgtg        <  1:1487005/140‑1 (MQ=255)

                                                                                                                                ctcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTgg       <  1:385100/140‑1 (MQ=255)

                                                                                                                                 tcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGa      <  2:462646/140‑1 (MQ=255)

                                                                                                                                 tcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGa      <  1:1294920/140‑1 (MQ=255)

                                                                                                                                 tcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGa      <  2:155312/140‑1 (MQ=255)

                                                                                                                                 tcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGa      <  1:1424866/140‑1 (MQ=255)

                                                                                                                                 tcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGa      <  2:335122/140‑1 (MQ=255)

                                                                                                                                 tcCTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGa      <  1:575889/140‑1 (MQ=255)

                                                                                                                                 tcCTTAGGCGTTTTAAGACGACGCTGGTGACTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGa      <  1:146323/140‑1 (MQ=255)

                                                                                                                                  ccTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGAt     <  2:679237/140‑1 (MQ=255)

                                                                                                                                   cTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGATc    <  1:336877/140‑1 (MQ=255)

                                                                                                                                   cTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGATc    <  2:385704/140‑1 (MQ=255)

                                                                                                                                   cTTAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGATc    <  2:255152/140‑1 (MQ=255)

                                                                                                                                     tAGGCGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGATCaa  <  1:1043593/140‑1 (MQ=255)

ATGGTACGGCGAATATTGCAGCGGTGAACGTCGCCATCGGCGGATTCCACATCAGCGTGCATACCAAAGCGGCTGATGACGATACCTTCATCAGGCTCGCCAAACAGATTGTCGTCGTAGTCGGGCTTCTCCTTAGACGTTTTAAGACGACGCTGGTGATTGGCGTTCACGCGGCGCTGCTGGCCTTTGGAGAGTTTATTTTTACTCAATCGTACAGACTCCTGGTCGCCCCTGATGGGCAAAACATCTATGATACACGCAATTGTGGATCAA  >  NC_000913/4391301‑4391573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A