Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,398,693	C→T	L428F (CTT→TTT)	mutL →	DNA mismatch repair protein MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,398,693	0	C	T	100.0%	118.9 / NA	34	L428F (CTT→TTT)	mutL	DNA mismatch repair protein MutL
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/22); total (12/22)

TCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACGGCAACATTTCACTTTTATCCTTGCCAGTGGCAGAACGTTGGCTGCGTCAGGCACAATT  >  NC_000913/4398566‑4398826

tcCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGa                                                                                                                              >  2:760/1‑137 (MQ=255)

tcCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGa                                                                                                                              <  1:760/137‑1 (MQ=255)

  cGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGt                                                                                                                         >  2:575508/1‑140 (MQ=255)

  cGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGt                                                                                                                         >  2:366801/1‑140 (MQ=255)

              ggCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGt                                                                                                             <  1:767945/140‑1 (MQ=255)

              ggCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGt                                                                                                             <  1:366801/140‑1 (MQ=255)

              ggCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGt                                                                                                             <  1:575508/140‑1 (MQ=255)

               gCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTc                                                                                                            <  1:1138463/140‑1 (MQ=255)

                 cGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCgg                                                                                                          <  1:1170291/140‑1 (MQ=255)

                 cGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCgg                                                                                                          <  2:184485/140‑1 (MQ=255)

                 cGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCgg                                                                                                          <  2:1176081/140‑1 (MQ=255)

                    aTGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTa                                                                                                       >  1:260633/1‑140 (MQ=255)

                    aTGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTa                                                                                                       <  2:1042189/140‑1 (MQ=255)

                    aTGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTa                                                                                                       <  2:673143/140‑1 (MQ=255)

                        gcAGCCAGGCTACCAGAAACAGCAAGGTGAAGCGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGa                                                                                                   <  1:1044000/140‑1 (MQ=255)

                         cAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCgg                                                                                                          >  2:935272/1‑132 (MQ=255)

                         cAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCgg                                                                                                          <  1:935272/132‑1 (MQ=255)

                          aGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACt                                                                                                 <  1:400421/140‑1 (MQ=255)

                          aGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACt                                                                                                 >  2:907514/1‑140 (MQ=255)

                              aGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCg                                                                                             <  1:232336/140‑1 (MQ=255)

                                           cAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACtg                                                                                <  1:907514/140‑1 (MQ=255)

                                           cAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACtg                                                                                <  2:260633/140‑1 (MQ=255)

                                              cAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGc                                                                             >  2:131968/1‑140 (MQ=255)

                                                         gtATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAgc                                                                  <  2:208353/140‑1 (MQ=255)

                                                            tCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAgcgc                                                                <  1:55906/139‑1 (MQ=255)

                                                            tCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAgcgc                                                                >  2:55906/1‑139 (MQ=255)

                                                            tCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGcgcg                                                               >  1:207579/1‑140 (MQ=255)

                                                               ccAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACg                                                            >  1:216327/1‑140 (MQ=255)

                                                                        gCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACGGCAACAttt                                                   <  2:216327/140‑1 (MQ=255)

                                                                                   cgcCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACGGCAACATTTCACTTTTATcc                                        >  2:1210554/1‑140 (MQ=255)

                                                                                       gATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACGGCAACATTTCACTTTTATCCTTGc                                    <  1:1210554/140‑1 (MQ=255)

                                                                                       gATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACGGCAACATTTCACTTTTATCCTTGc                                    <  1:885660/140‑1 (MQ=255)

                                                                                                                    aGGAACCTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACGGCAACATTTCACTTTTATCCTTGCCAGTGGCAGAACGTTGGCTGCGTCAGGca       <  2:207579/140‑1 (MQ=255)

                                                                                                                         ccTGCATTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCCGGAGCGCGACGGCAACATTTCACTTTTATCCTTGCCAGTGGCAGAACGTTGGCTGCGTCAGGCACAAtt  >  1:232063/1‑140 (MQ=255)

TCCGGCTGCCCCCTGGCCGAATGCGCAGCCAGGCTACCAGAAACAGCAAGGTGAAGTGTATCGCCAGCTTTTGCAAACGCCCGCGCCGATGCAAAAATTAAAAGCGCCGGAACCGCAGGAACCTGCACTTGCGGCGAACAGTCAGAGTTTTGGTCGGGTACTGACTATCGTCCATTCCGACTGTGCGTTGCTGGAGCGCGACGGCAACATTTCACTTTTATCCTTGCCAGTGGCAGAACGTTGGCTGCGTCAGGCACAATT  >  NC_000913/4398566‑4398826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A