Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A47 F60 I1 R1 14 120.0 4052012 98.6% 3995283 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C95.7% 209.7 / 2.8 69intergenic (+586/+56)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (33/33);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01
*NC_0009134,296,3802.G95.6% 209.0 / 2.9 69intergenic (+586/+56)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base G (33/32);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

GTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCA  >  NC_000913/4296308‑4296503
                                                                             ||                                                                                                                           
gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg                                                            >  1:1963825/1‑140 (MQ=255)
 tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTgg                                                           >  1:1677876/1‑140 (MQ=255)
   ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCATCTTATAAGGCCTATCTTAACCGtctgtc                                                         >  1:720029/1‑135 (MQ=11)
   ttATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCATGATGCGAGGCTTGGCGCGTCTTATGAGGCCGATCTTAACCGTTGGtt                                                         >  2:862210/1‑140 (MQ=11)
    tATCAGGCCTACGCCAGACAGCTCAATAGCCTGATTTTGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTa                                                        <  1:862210/140‑1 (MQ=11)
              aCGCCAGACAGCGCACTAGCCTCATCTAGTGTGATTTCGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  1:606195/140‑1 (MQ=14)
              aCGCCAGACAGCGCACTAGCCTCATCTAGCGTGATTTCGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  1:546869/140‑1 (MQ=14)
              aCGCCAGACAGCGCAATCGCCTTATTTAGCGAGATTTTGGAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  2:732915/140‑1 (MQ=14)
              aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  2:1312907/140‑1 (MQ=255)
              aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  1:344001/140‑1 (MQ=255)
              aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  1:1985540/140‑1 (MQ=255)
               cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGTCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc                                             >  2:1155193/1‑140 (MQ=255)
                gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAct                                            >  2:24865/1‑140 (MQ=255)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                        >  1:1629352/1‑140 (MQ=255)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                        >  1:344848/1‑140 (MQ=255)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                        >  1:213714/1‑140 (MQ=255)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                        >  1:1365325/1‑140 (MQ=255)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTc                                        >  1:1643039/1‑140 (MQ=255)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAAGCCTATCATAAACGATGGTTAGTTTTTCAAACTCTTc                                        >  1:596039/1‑140 (MQ=14)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCCCGTCTTATAAGGCCTAACTTAACCGATGGTCAATTTTTCAAACTCTTc                                        >  2:648830/1‑140 (MQ=14)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  2:1550716/134‑1 (MQ=255)
                    gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              >  1:1550716/1‑134 (MQ=255)
                         gagacaggCCTGATTTAGCGTGATTCTGTGGGTCGGAGAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   <  1:978122/133‑1 (MQ=17)
                         cgcAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              <  1:1241690/129‑1 (MQ=14)
                         cgcAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                              >  2:1241690/1‑129 (MQ=14)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                 >  2:496036/1‑126 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc                                                 <  1:496036/126‑1 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   >  1:1061592/1‑140 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   >  1:31410/1‑140 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   <  1:289295/140‑1 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   >  1:271204/1‑140 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   >  1:1781123/1‑140 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   >  2:1748537/1‑140 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   <  1:1396491/140‑1 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   >  1:1103562/1‑140 (MQ=255)
                         cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCGTATCAGGCCTATATTAGCCGTTGGTTAATTTTTCAAACTCTTCACAcc                                   >  2:892543/1‑140 (MQ=17)
                          gcAATAGCCTGATTTCGCGTGATTTCGTAGGTCGGATTAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                  <  1:648830/140‑1 (MQ=17)
                          gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                  <  1:24865/140‑1 (MQ=255)
                          gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCATTGATTAATTTTTTAAACTCTTCACAccc                                  >  1:749107/1‑140 (MQ=17)
                          gcAATAGCCTGATTTAGCGTGAATTTGTAGGTCGGTTAACGCGTT‑‑T‑‑ACTCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                  <  2:809671/140‑1 (MQ=18)
                          gcAATAGCCTGATTTAGCGTGAATTTGTAGGCCGGCTAAAGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc                                  <  2:596039/140‑1 (MQ=17)
                              tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa                              >  1:1671880/1‑140 (MQ=255)
                                   tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGTCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                         <  1:1155193/140‑1 (MQ=255)
                                   tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                         <  1:366792/140‑1 (MQ=255)
                                   tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                         <  1:1748537/140‑1 (MQ=255)
                                   tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGATGGTTAATTTTTCAAACTCTTAACACCCGGTATCaaa                         >  1:965528/1‑140 (MQ=18)
                                   tGATTTAGCGTGATTTTGTAGGGCGAATAAGGCGTT‑‑TCCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                         <  1:573058/140‑1 (MQ=18)
                                   tGATTTAGCGTGATTTTGTAGGACGGATAGGGCGTT‑‑TCCG‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa                         <  1:892543/140‑1 (MQ=18)
                                      tttAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAccc                      <  1:1438386/140‑1 (MQ=255)
                                        tAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                    <  1:472217/140‑1 (MQ=255)
                                        tAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                    <  2:213714/140‑1 (MQ=255)
                                        tAGCGTGATTTTGTACGTCGGAGAAAGCGCT‑‑T‑‑ACGCCTCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt                    <  1:575381/140‑1 (MQ=21)
                                           cGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAGCGTTGGTTCATTATTCCCACTCTTCGCACCGCGTATCAAAcctgtctc                 >  2:978122/1‑134 (MQ=14)
                                           cGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa                 >  1:1821106/1‑140 (MQ=255)
                                             tGATTTTGTAGGTCGGAGAAGGAGTT‑‑T‑‑ACGCCGCAGCCGTCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa               <  2:749107/140‑1 (MQ=21)
                                               aTTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                           <  1:296404/126‑1 (MQ=255)
                                               aTTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCa                           >  2:296404/1‑126 (MQ=255)
                                                ttttGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg            >  2:6936/1‑140 (MQ=255)
                                                  ttGTAGGTGGGATAAGGTGTT‑‑T‑‑ACGCCGCACCCGATATCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt          <  2:985173/140‑1 (MQ=25)
                                                  ttGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt          <  2:31410/140‑1 (MQ=255)
                                                  ttGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt          <  2:1671880/140‑1 (MQ=255)
                                                  ttGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt          <  2:1643039/140‑1 (MQ=255)
                                                  ttGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt          <  2:1379252/140‑1 (MQ=255)
                                                  ttGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt          <  1:420461/140‑1 (MQ=255)
                                                   tGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTc         >  1:148501/1‑140 (MQ=255)
                                                   tGTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTc         >  1:1086755/1‑140 (MQ=255)
                                                    gTAGGTCGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa        <  2:131823/140‑1 (MQ=255)
                                                       ggTCGGATGAGGCGTTAGC‑‑G‑‑CCGCATCCGACGTCACTGCCTGATGCGATGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt     <  2:965528/140‑1 (MQ=25)
                                                          cGGATAAGGCGTT‑‑T‑‑ACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCGCACCCGGAATCAAACCCTTCCCTACAGCTCAGGTTCa  >  1:985173/1‑140 (MQ=25)
                                                                             ||                                                                                                                           
GTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTT‑‑T‑‑A‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCA  >  NC_000913/4296308‑4296503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A