Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F60 I1 R1
|
18 |
54.8 |
1909210 |
98.2% |
1874844 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,296,381 |
+GC |
intergenic (+587/+55) |
gltP → / ← yjcO |
glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,296,380 | 1 | . | C | 91.3%
| 65.9
/ 0.3
| 23 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base C (12/9); total (12/11) |
| Fisher's exact test for biased strand distribution p-value = 2.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| * | NC_000913 | 4,296,380 | 2 | . | G | 91.3%
| 65.8
/ 0.4
| 23 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (12/9); total (12/11) |
| Fisher's exact test for biased strand distribution p-value = 2.17e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAC > NC_000913/4296305‑4296505
||
cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCg > 1:49148/1‑140 (MQ=9)
tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTgg > 2:908273/1‑140 (MQ=255)
tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTgg > 2:855133/1‑140 (MQ=255)
aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTAATGCGACGCTTGGCGCTTCTTATCATGCCTTTCTTAA‑CCGTTGGTTaa > 2:263257/1‑140 (MQ=11)
ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTc > 1:858751/1‑140 (MQ=255)
cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAAc > 2:392087/1‑140 (MQ=255)
cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCaaa < 2:474389/129‑1 (MQ=255)
cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCaaa > 1:474389/1‑129 (MQ=255)
cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACAcc > 2:829047/1‑140 (MQ=255)
tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGGTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:18000/140‑1 (MQ=255)
tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:564254/140‑1 (MQ=255)
tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa > 1:18881/1‑140 (MQ=255)
tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:823439/140‑1 (MQ=255)
tGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTATACA‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:365345/140‑1 (MQ=21)
tAGCGTGATTTTGTAAGTCGGATAATGCGCTT‑‑ACGC‑‑CCCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt < 1:410481/140‑1 (MQ=18)
gCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTATTTTTTCACACTATTAACACCCCGTATCAAACCCTTcc > 1:365345/1‑140 (MQ=18)
ttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt < 2:18881/140‑1 (MQ=255)
ttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt < 2:621248/140‑1 (MQ=255)
ttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCt < 1:218558/140‑1 (MQ=255)
ggTCGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt < 1:516750/140‑1 (MQ=255)
cGGATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCa > 2:635631/1‑140 (MQ=255)
gATAAGGCGTTT‑‑ACGC‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa > 1:635824/1‑140 (MQ=25)
gATAAGGCGTTT‑‑A‑‑CGACGCATCCGACATCGATGCCTGGTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc < 1:421715/140‑1 (MQ=37)
||
CGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAA‑CCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAC > NC_000913/4296305‑4296505
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
