Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A30 F60 I1 R1	16	47.7	1604874	98.7%	1584010	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,715,038	T→G	I453L (ATC→CTC)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,715,038	0	T	G	100.0%	176.1 / NA	48	I453L (ATC→CTC)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/31); total (17/31)

CCACTTGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGATCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCG  >  NC_000913/3714903‑3715159

ccacTTGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGcacc                                                                                                                       <  2:11765/140‑1 (MQ=255)

    ttGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCAccacca                                                                                                                   <  2:439738/140‑1 (MQ=255)

       cTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCt                                                                                                                <  2:9339/140‑1 (MQ=255)

        ttCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTc                                                                                                               <  1:651503/140‑1 (MQ=255)

              ggCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGttt                                                                                                         >  1:7977/1‑140 (MQ=255)

                 aCCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTg                                                                                                      <  2:76214/140‑1 (MQ=255)

                 aCCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTg                                                                                                      <  2:638020/140‑1 (MQ=255)

                  ccAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGc                                                                                                     >  2:529362/1‑140 (MQ=255)

                     gATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGAATTGAGAGAGCAACACCAGCTCCGGTTTTTGCCAg                                                                                                  >  2:277192/1‑140 (MQ=255)

                             ttACTGTAATCACCGGTGATGGCGAGATCATGACGATCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGAt                                                                                          <  2:269635/140‑1 (MQ=255)

                             ttACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGAt                                                                                          <  1:558983/140‑1 (MQ=255)

                              tACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGc                                                                                                     >  2:778658/1‑128 (MQ=255)

                              tACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGc                                                                                                     <  1:778658/128‑1 (MQ=255)

                               aCTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACGACCAGCTCCGGTTTCTGCCAGGAACGGATCa                                                                                        >  2:360359/1‑140 (MQ=255)

                                  gTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGc                                                                                     <  2:7977/140‑1 (MQ=255)

                                  gTAATCACCGGTCATGGTGAGATCAATACTCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGc                                                                                     <  1:277192/140‑1 (MQ=255)

                                  gTAATCACCGGTCATGCTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGc                                                                                     <  2:480852/140‑1 (MQ=255)

                                     aTCACCGGTCATGGTGAGATCATTACGCTTAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGAt                                                                                  <  1:378029/140‑1 (MQ=255)

                                     aTCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGAt                                                                                  <  2:568505/140‑1 (MQ=255)

                                          cGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTa                                                                             >  2:679269/1‑140 (MQ=255)

                                           ggTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTgg                                                                               <  1:526967/137‑1 (MQ=255)

                                           ggTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTgg                                                                               >  2:526967/1‑137 (MQ=255)

                                               aTGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCtg                                                                        <  1:6354/140‑1 (MQ=255)

                                                 ggTGAGATCATTACGCTCAAAATAGGTAGTCCCAGGCAGTACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCtgat                                                                      <  2:269788/140‑1 (MQ=255)

                                                 ggTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCtgat                                                                      <  1:529362/140‑1 (MQ=255)

                                                 ggTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTAGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCtgat                                                                      <  2:757143/140‑1 (MQ=255)

                                                  gTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCtgatg                                                                     <  1:631104/140‑1 (MQ=255)

                                                    gagaTCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCtgatgat                                                                   >  1:104530/1‑140 (MQ=255)

                                                           ttACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTaaa                                                            >  1:610320/1‑140 (MQ=255)

                                                            tACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAg                                                           >  1:763942/1‑140 (MQ=255)

                                                            tACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCAAGGAACAGGCGATCGGTATCCTGATGATGAGTAAAg                                                           >  2:301497/1‑140 (MQ=255)

                                                            tACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGAACGGATCCGGCGATTGGTATCATGATGATGAGTAAAg                                                           >  1:269788/1‑140 (MQ=255)

                                                                 tCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGc                                                      >  2:165174/1‑140 (MQ=255)

                                                                 tCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGc                                                      >  1:721993/1‑140 (MQ=255)

                                                                    aaaGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCAcc                                                   <  1:360359/140‑1 (MQ=255)

                                                                      agagGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCAccgc                                                 <  1:165174/140‑1 (MQ=255)

                                                                        agGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCAccgccc                                               <  2:610320/140‑1 (MQ=255)

                                                                             gTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCcca                                          <  2:721993/140‑1 (MQ=255)

                                                                             gTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGAACAGGCGATCGGTATCCCGATGATGACTAAACTTGGCACCGCCCGCcca                                          >  2:281163/1‑140 (MQ=255)

                                                                               cGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGTTATCCTGATGATGAGTCAAGTAGGCACCGCCCGCccacc                                        >  2:279815/1‑140 (MQ=255)

                                                                                    gcagAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATa                                   <  2:552203/140‑1 (MQ=255)

                                                                                         aCGATATCCGCGTGTATTGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACg                              <  1:301497/140‑1 (MQ=255)

                                                                                                       ttttGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAAt                >  2:769535/1‑140 (MQ=255)

                                                                                                        tttGCCGCCGCCGTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATg               <  1:769535/140‑1 (MQ=255)

                                                                                                             cgccgccgTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGt          <  1:679269/140‑1 (MQ=255)

                                                                                                                cgccgTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCa       <  1:279815/140‑1 (MQ=255)

                                                                                                                  ccgTCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATa     <  2:734849/140‑1 (MQ=255)

                                                                                                                     tCCAGAAGCATTCAGAGAGCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCg  <  2:305897/140‑1 (MQ=255)

CCACTTGCTTCATCGGCACCAGATGCTGATTACTGTAATCACCGGTCATGGTGAGATCATTACGCTCAAAAGAGGTAGTCGCAGGCAGAACGATATCCGCGTGTTTTGCCGCCGCCGTCCAGAAGCATTCAGAGATCACCACCAGCTCCGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCG  >  NC_000913/3714903‑3715159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A