Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A42 F60 I1 R1	18	124.9	4351958	99.3%	4321494	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,577,264	T→A	D232V (GAT→GTT)	mcrC ←	5‑methylcytosine‑specific restriction enzyme subunit McrC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,577,264	0	T	A	100.0%	521.1 / NA	147	D232V (GAT→GTT)	mcrC	5‑methylcytosine‑specific restriction enzyme subunit McrC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (71/76); total (71/76)

Only 100 of 153 total aligned reads displayed.

TTTGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGATCCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGA  >  NC_000913/4577127‑4577399

tttGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAAcc                                                                                                                                       >  1:1840563/1‑140 (MQ=255)

tttGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAAcc                                                                                                                                       >  1:1889756/1‑140 (MQ=255)

 ttGCGAATGAAATTTTTTTGTTCCCATTCGTTGTGTAAATATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCg                                                                                                                                      <  2:678850/140‑1 (MQ=255)

 ttGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCg                                                                                                                                      <  1:1961875/140‑1 (MQ=255)

  tGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCg                                                                                                                                      >  2:449257/1‑139 (MQ=255)

  tGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGa                                                                                                                                     >  1:360126/1‑140 (MQ=255)

  tGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTAAGTGTCCATTCGAGGTTACAAATTAAGTGACTGAACCGa                                                                                                                                     >  2:784537/1‑140 (MQ=255)

   gCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGat                                                                                                                                    <  2:1257375/140‑1 (MQ=255)

   gCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGat                                                                                                                                    <  1:1339091/140‑1 (MQ=255)

        tGAAATTTTTCTGTTCCCATTCGTCGTGAAATAATCCTCCTATAGTATCTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACt                                                                                                                               <  2:647397/140‑1 (MQ=255)

        tGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACt                                                                                                                               <  2:2037662/140‑1 (MQ=255)

        tGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACt                                                                                                                               <  1:72035/140‑1 (MQ=255)

        tGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACt                                                                                                                               <  2:454489/140‑1 (MQ=255)

        tGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACt                                                                                                                               <  1:1382088/140‑1 (MQ=255)

        tGAAATTTTTCTGTTCCCATTCGTCATGACAAAATGCCCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACt                                                                                                                               <  1:575193/140‑1 (MQ=255)

                ttCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCAt                                                                                                                       <  1:1146491/140‑1 (MQ=255)

                 tCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATc                                                                                                                      <  1:2066188/140‑1 (MQ=255)

                 tCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATc                                                                                                                      <  1:1513473/140‑1 (MQ=255)

                 tCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATc                                                                                                                      <  2:1357686/140‑1 (MQ=255)

                  cTGTTCCCATTCGTCGTGAAAAAATTCTCGTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATcc                                                                                                                     <  1:784537/140‑1 (MQ=255)

                     ttCCCATTCGTCTTTAAAAAATGCTCTTATAGTGTTGGTTGTCTTCGGTAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGAAACAAATTAAGAGACTGAAGCGATATACTCGATCCATCCCAt                                                                                                                  >  2:1035956/1‑140 (MQ=255)

                     ttCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCAt                                                                                                                  >  2:327292/1‑140 (MQ=255)

                     ttCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCAt                                                                                                                  >  1:1295828/1‑140 (MQ=255)

                      tCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCAtt                                                                                                                 <  1:75372/140‑1 (MQ=255)

                         cATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTa                                                                                                              <  1:1880295/140‑1 (MQ=255)

                           ttCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTaaa                                                                                                            <  1:1751237/140‑1 (MQ=255)

                               tcgtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAag                                                                                                        <  2:1889756/140‑1 (MQ=255)

                               tcgtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAag                                                                                                        >  2:1957382/1‑140 (MQ=255)

                               tcgtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAag                                                                                                        >  1:1332447/1‑140 (MQ=255)

                               tcgtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAag                                                                                                        <  2:1840563/140‑1 (MQ=255)

                               tcgtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAag                                                                                                        <  1:410528/140‑1 (MQ=255)

                                 gtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAagag                                                                                                      <  2:1558121/140‑1 (MQ=255)

                                 gtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAagag                                                                                                      <  2:1170341/140‑1 (MQ=255)

                                 gtGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAagag                                                                                                      <  1:1611291/140‑1 (MQ=255)

                                      aaaaTGCTCTTATAGTATTTGGGGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGt                                                                                                 <  1:960757/140‑1 (MQ=255)

                                      aaaaTGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGt                                                                                                 <  1:327292/140‑1 (MQ=255)

                                                 ataGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAg                                                                                      >  2:169441/1‑140 (MQ=255)

                                                 ataGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGAGGGTTGTGTTTGCAg                                                                                      >  1:792291/1‑140 (MQ=255)

                                                      aTTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATAATCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCa                                                                                       >  2:1842/1‑134 (MQ=255)

                                                      aTTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATAATCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCa                                                                                       <  1:1842/134‑1 (MQ=255)

                                                       tttGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCGCATTTTAGATAGGAACGGGTTTTGTTTGCAGACGTTa                                                                                >  1:642967/1‑140 (MQ=255)

                                                       tttGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGt                                                                                                 <  1:2119175/123‑1 (MQ=255)

                                                       tttGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGt                                                                                                 >  2:2119175/1‑123 (MQ=255)

                                                       tttGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTa                                                                                >  1:1180504/1‑140 (MQ=255)

                                                       tttGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATAATCGATGCAGCCCCTTTCAAATAAGACCGGGGTGTGTTTGCAGACGTTa                                                                                >  2:725408/1‑140 (MQ=255)

                                                         tGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTTGATGCATCACATTTTAGATAAGAGCGGGTTGTGTTTGCAGACGTTAAt                                                                              >  2:854710/1‑140 (MQ=255)

                                                         tGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAAt                                                                              >  2:1789636/1‑140 (MQ=255)

                                                         tGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAAt                                                                              >  2:1869171/1‑140 (MQ=255)

                                                         tGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAAt                                                                              >  1:1157031/1‑140 (MQ=255)

                                                         tGGCGTCAACGATAAGCATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAACTTAAGTGACTGAACCGATATACTGGATGCATCTCATTTCAAATAAGAGCCGGTTACGTTTGCAGACGTTAAt                                                                              >  2:930578/1‑140 (MQ=255)

                                                               cAATGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAGATAAGAGGGGGATGTGTTTGCAGACGTTAATTCacga                                                                        >  1:785077/1‑140 (MQ=255)

                                                               cAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCacga                                                                        <  1:169441/140‑1 (MQ=255)

                                                               cAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCacga                                                                        >  1:1204043/1‑140 (MQ=255)

                                                               cAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCacga                                                                        >  2:1170310/1‑140 (MQ=255)

                                                               cAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGAGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGACCGGGTTGTGTTTGGAGACTGTAATTCacga                                                                        >  2:1930105/1‑140 (MQ=255)

                                                                           ttttttCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCa                                                            >  1:1261248/1‑140 (MQ=255)

                                                                                   gatgaGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCa                                                                   <  2:383733/125‑1 (MQ=255)

                                                                                   gatgaGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCa                                                                   >  1:383733/1‑125 (MQ=255)

                                                                                   gatgaGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGaaa                                                    >  1:1183724/1‑140 (MQ=255)

                                                                                   gatgaGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGaaa                                                    >  1:1415011/1‑140 (MQ=255)

                                                                                   gatgaGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGaaa                                                    >  2:1724416/1‑140 (MQ=255)

                                                                                    atgaGCGAATGGTGATGTCAGTTTCCATTCGTGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAAc                                                   <  2:792291/140‑1 (MQ=255)

                                                                                        gCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGAAGTTAATTTACGACGGCAAAATACATAAAGAAACtttt                                               >  1:1046327/1‑140 (MQ=255)

                                                                                         cGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTg                                              >  2:304745/1‑140 (MQ=255)

                                                                                         cGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTg                                              >  2:1409880/1‑140 (MQ=255)

                                                                                         cGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTg                                              >  2:1469046/1‑140 (MQ=255)

                                                                                         cGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTg                                              >  1:1456099/1‑140 (MQ=255)

                                                                                          gAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGa                                             <  2:2051461/140‑1 (MQ=255)

                                                                                          gAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGa                                             <  1:1469046/140‑1 (MQ=255)

                                                                                          gAATGGTGATGTCAGTCTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGa                                             <  2:1323818/140‑1 (MQ=255)

                                                                                          gAATGGGGATGTCAGTTTCCATTCGGGGTTACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGa                                             <  1:884069/140‑1 (MQ=255)

                                                                                           aaTGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGAt                                            <  2:46139/140‑1 (MQ=255)

                                                                                           aaTGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGAt                                            <  1:1391228/140‑1 (MQ=255)

                                                                                           aaTGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGAt                                            <  2:360126/140‑1 (MQ=255)

                                                                                            aTGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATa                                           <  2:1360793/140‑1 (MQ=255)

                                                                                               gTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAg                                        >  1:2053731/1‑140 (MQ=255)

                                                                                                  aTGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTaa                                     >  2:221948/1‑140 (MQ=255)

                                                                                                   tGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAAt                                    <  2:1969270/140‑1 (MQ=255)

                                                                                                      cAGTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTAAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGAc                                 <  1:470634/140‑1 (MQ=255)

                                                                                                        gTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACAt                               >  1:36470/1‑140 (MQ=255)

                                                                                                        gTTTCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACAt                               >  1:170870/1‑140 (MQ=255)

                                                                                                         tttCCATTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACAtc                              >  1:1940602/1‑140 (MQ=255)

                                                                                                              aTTCGAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATCCATAAAGCAACATTCGATAAAATAATGACATCTCttt                         >  1:757905/1‑140 (MQ=255)

                                                                                                                  gAGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCg                     <  1:1992643/140‑1 (MQ=255)

                                                                                                                   aGGTAACAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGt                    >  1:1362412/1‑140 (MQ=255)

                                                                                                                       aaCAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGtttct                >  1:69732/1‑140 (MQ=255)

                                                                                                                        aCAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAAATCATAAAGTAACATTTCATAAAGTACTGACTTCTCTTTTTCGtttctt               >  2:857030/1‑140 (MQ=255)

                                                                                                                         cAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGtttcttt              <  2:58191/140‑1 (MQ=255)

                                                                                                                         cAAATTAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAAATTTTGATAAAGTAAGGACATCGCTTTTTCGtttcttt              >  1:1053705/1‑140 (MQ=255)

                                                                                                                              tAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGGCAGCTCTTTATCGTTTCTTTCaaaa         >  1:892273/1‑140 (MQ=255)

                                                                                                                              tAAGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCaaaa         >  2:389831/1‑140 (MQ=255)

                                                                                                                                aGTGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATc       <  1:304745/140‑1 (MQ=255)

                                                                                                                                  tGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCAt     <  2:1295828/140‑1 (MQ=255)

                                                                                                                                  tGACTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCAt     <  2:1934162/140‑1 (MQ=255)

                                                                                                                                    aCTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCaa           >  2:1201133/1‑132 (MQ=255)

                                                                                                                                    aCTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCaa           <  1:1201133/132‑1 (MQ=255)

                                                                                                                                    aCTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAg   >  1:1913716/1‑140 (MQ=255)

                                                                                                                                    aCTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTGTTCGTTTCTTTCAAAATCATAg   >  2:740423/1‑140 (MQ=255)

                                                                                                                                    aCTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAATAATGACATCTGTTGTTCGTTGCTTTCAAAATCATAg   >  1:633664/1‑140 (MQ=255)

                                                                                                                                     cTGAACCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGa  <  2:1752848/140‑1 (MQ=255)

TTTGCGAATGAAATTTTTCTGTTCCCATTCGTCGTGAAAAAATGCTCTTATAGTATTTGGCGTCAACGATAAGTATTTTTTCTGATGAGCGAATGGTGATGTCAGTTTCCATTCGAGGTAACAAATTAAGTGACTGATCCGATATACTCGATGCATCCCATTTTAAATAAGAGCGGGTTGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGA  >  NC_000913/4577127‑4577399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A