Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F60 I1 R1	12	98.5	3402390	98.5%	3351354	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,033,551	G→A	V163M (GTG→ATG)	idi →	isopentenyl‑diphosphate Delta‑isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,033,551	0	G	A	98.7%	260.8 / NA	77	V163M (GTG→ATG)	idi	isopentenyl‑diphosphate Delta‑isomerase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (41/35); minor base C (0/1); total (41/36)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAA  >  NC_000913/3033421‑3033683

ggTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAgg                                                                                                                             >  2:146218/1‑140 (MQ=255)

     ttGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATGGATGCCACGCCGCGGGGGTTCAGTCCGTGGATGATGATGCAGGCGACa                                                                                                                        >  2:658971/1‑140 (MQ=255)

      tGCCGCACGCAACACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACaa                                                                                                                       <  2:378013/140‑1 (MQ=255)

           cacgcacCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATcgc                                                                                                                  >  1:1624014/1‑140 (MQ=255)

            acgcacCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATcgcg                                                                                                                 >  2:1478068/1‑140 (MQ=255)

             cgcacCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTCATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGa                                                                                                                <  2:1036323/140‑1 (MQ=255)

                accacTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGc                                                                                                             <  1:146218/140‑1 (MQ=255)

                  cacTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGc                                                                                                             <  2:1647157/138‑1 (MQ=255)

                  cacTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGc                                                                                                             >  1:1647157/1‑138 (MQ=255)

                          cGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGa                                                                                                         <  2:1611124/134‑1 (MQ=255)

                          cGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGa                                                                                                         >  1:1611124/1‑134 (MQ=255)

                          cGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGa                                                                                                   >  1:1035651/1‑140 (MQ=255)

                          cGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGa                                                                                                   <  2:423581/140‑1 (MQ=255)

                          cGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGa                                                                                                   >  1:1240641/1‑140 (MQ=255)

                            ttACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACg                                                                                                    <  2:1127154/137‑1 (MQ=255)

                            ttACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACg                                                                                                    >  1:1127154/1‑137 (MQ=255)

                            ttACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGAtt                                                                                                 >  2:911810/1‑140 (MQ=255)

                              aCAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTAt                                                                                               <  2:1240641/140‑1 (MQ=255)

                               cAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATc                                                                                              <  1:130787/140‑1 (MQ=255)

                               cAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATc                                                                                              <  1:962778/140‑1 (MQ=255)

                                  aTCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGa                                                                                                   >  1:1464937/1‑132 (MQ=255)

                                  aTCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGa                                                                                                   <  2:1464937/132‑1 (MQ=255)

                                  aTCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGc                                                                                           >  1:1639763/1‑140 (MQ=255)

                                      atgatgatgAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCAttt                                                                                       >  1:1454714/1‑140 (MQ=255)

                                         atgatgaAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTAcc                                                                                    <  2:405029/140‑1 (MQ=255)

                                         atgatgaAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTAcc                                                                                    >  1:359478/1‑140 (MQ=255)

                                            atgaAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAg                                                                                 >  2:1529608/1‑140 (MQ=255)

                                            atgaAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAg                                                                                 >  1:75944/1‑140 (MQ=255)

                                            atgaAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATCCAGGCGACAAATAGCGCAGCCAGAAGACGAATATCTGCATTTACCCAg                                                                                 >  2:576796/1‑140 (MQ=255)

                                             tgaATTGATGGATTAGGCATGTCGTGATTTACCAGCTGTGTTACACGGAATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAATTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGc                                                                                <  2:640475/140‑1 (MQ=255)

                                                aGTGATGGATTATCAATGGTGTGATTTAGGAGATGTACTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTta                                                                             <  1:658971/140‑1 (MQ=255)

                                                 gTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTta                                                                             <  1:953998/139‑1 (MQ=255)

                                                 gTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTta                                                                             >  2:953998/1‑139 (MQ=255)

                                                  tGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTtaaa                                                                           <  1:842750/140‑1 (MQ=255)

                                                      ggATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCAt                                                                                         >  1:1597512/1‑122 (MQ=255)

                                                      ggATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCAt                                                                                         <  2:1597512/122‑1 (MQ=255)

                                                           aTCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAAc                                                                  >  1:307764/1‑140 (MQ=255)

                                                            tCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATTTGCGTTTACCCAGATTAAATAAAAAAAcc                                                                 >  1:805902/1‑140 (MQ=255)

                                                            tCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATTTGCATTCACCCAGTTTAAATAAAAAAAcc                                                                 >  1:817570/1‑140 (MQ=255)

                                                             cAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAAccc                                                                <  1:1478068/140‑1 (MQ=255)

                                                                 ngtttgATTTAGCAGATGTATTACACGGTATTTATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGAc                                                            <  2:248448/136‑1 (MQ=255)

                                                                 ggTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGAc                                                            <  2:1514654/140‑1 (MQ=255)

                                                                 ggTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATGATCTGCATTTAGCCGGCTTAAATAAAAAAACCCCGAc                                                            >  1:477376/1‑140 (MQ=255)

                                                                     tgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAttt                                                        >  1:149879/1‑140 (MQ=255)

                                                                      gATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACa                                                           <  2:1403227/136‑1 (MQ=255)

                                                                      gATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACa                                                           >  1:1403227/1‑136 (MQ=255)

                                                                       aTTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCAATTAACCAGCTTAGATAAAAAAACCCCGACATTTGc                                                      >  2:506122/1‑140 (MQ=255)

                                                                         ttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCg                                                    <  2:149879/140‑1 (MQ=255)

                                                                           aGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCggg                                                  <  1:911810/140‑1 (MQ=255)

                                                                                aTGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                             <  2:1639763/140‑1 (MQ=255)

                                                                                     ttACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                             <  1:224375/135‑1 (MQ=255)

                                                                                     ttACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                             >  2:224375/1‑135 (MQ=255)

                                                                                             tATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                             >  2:89348/1‑127 (MQ=255)

                                                                                             tATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                             <  1:89348/127‑1 (MQ=255)

                                                                                              aTTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAAt                               >  2:1398898/1‑140 (MQ=255)

                                                                                               ttGATGCCACGCCGTGGCCGTTAAGTCCGTGCATGCTGATGCAGGCGATAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATg                              <  2:477376/140‑1 (MQ=255)

                                                                                                tGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                             <  2:205546/124‑1 (MQ=255)

                                                                                                tGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTtgt                                             >  1:205546/1‑124 (MQ=255)

                                                                                                  aTGCCACGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCtt                           >  2:1410761/1‑140 (MQ=255)

                                                                                                       aCGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAtttt                      >  1:1022791/1‑140 (MQ=255)

                                                                                                       aCGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAtttt                      >  2:1075919/1‑140 (MQ=255)

                                                                                                       aCGCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTAtttt                      >  2:1090329/1‑140 (MQ=255)

                                                                                                         gCCGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAGACCCCGACGTTTACGGGGGTTGTGAACATAGCGTAATGCTTATTTTAc                    >  2:727358/1‑140 (MQ=255)

                                                                                                           cGTGGGCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGGTGTGAGCATAACGTAATGCTTATTTTACCg                  <  2:57102/140‑1 (MQ=255)

                                                                                                               ggCGTTCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACg              >  2:1376352/1‑140 (MQ=255)

                                                                                                                   ttCAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATc          >  1:1651171/1‑140 (MQ=255)

                                                                                                                     cAGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGc        >  1:1445338/1‑140 (MQ=255)

                                                                                                                      aGTCCGTGGATGATGTTGCAGGGGATAAACCGCGAAGTCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGcc       <  1:576796/140‑1 (MQ=255)

                                                                                                                      aGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGcc       <  2:1651171/140‑1 (MQ=255)

                                                                                                                      aGTCCGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGcc       <  1:1398898/140‑1 (MQ=255)

                                                                                                                         ccGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg    >  1:1102375/1‑140 (MQ=255)

                                                                                                                         ccGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCggg    <  1:1529608/140‑1 (MQ=255)

                                                                                                                          cGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGa   >  2:1212314/1‑140 (MQ=255)

                                                                                                                          cGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGa   >  2:1611523/1‑140 (MQ=255)

                                                                                                                          cGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGa   <  2:1022791/140‑1 (MQ=255)

                                                                                                                          cGTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGa   >  2:1471856/1‑140 (MQ=255)

                                                                                                                           gTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGaa  <  2:359478/140‑1 (MQ=255)

                                                                                                                           gTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGaa  <  1:1212314/140‑1 (MQ=255)

                                                                                                                           gTGGATGATGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGaa  <  1:1603214/140‑1 (MQ=255)

GGTATTTGCCGCACGCACCACTAGTGCGTTACAGATCAATGATGATGAAGTGATGGATTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAA  >  NC_000913/3033421‑3033683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A