Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A21 F60 I1 R1	12	98.5	3402390	98.5%	3351354	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,214,853	T→C	S192P (TCG→CCG)	metA →	homoserine O‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,214,853	0	T	C	100.0%	357.7 / NA	95	S192P (TCG→CCG)	metA	homoserine O‑succinyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (45/50); total (45/50)

GCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCC  >  NC_000913/4214718‑4214987

gCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGGGTGGCTTCGATCATGCATTCCTGGCACCGCATCcgcg                                                                                                                                    >  1:783356/1‑140 (MQ=255)

 ccGCGCTCAATATCCTCTACGGAATCCCTTAGCAAACCCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCgcgc                                                                                                                                   <  1:519740/140‑1 (MQ=255)

  cgcgCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCt                                                                                                                                  >  1:43361/1‑140 (MQ=255)

     gcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCcgcg                                                                                                                                    <  2:911246/135‑1 (MQ=255)

     gcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCcgcg                                                                                                                                    >  1:911246/1‑135 (MQ=255)

     gcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCcgcg                                                                                                                                    >  2:232992/1‑135 (MQ=255)

     gcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCcgcg                                                                                                                                    <  1:232992/135‑1 (MQ=255)

     gcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCt                                                                                                                                  <  1:1336454/137‑1 (MQ=255)

     gcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCt                                                                                                                                  >  2:1336454/1‑137 (MQ=255)

     gcTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATg                                                                                                                               >  1:1374872/1‑140 (MQ=255)

       tCAATATCCTCTACGTCATTCCCAACCAACCTCGCACAGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCt                                                                                                                             <  1:451721/140‑1 (MQ=255)

       tCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCt                                                                                                                             <  2:901192/140‑1 (MQ=255)

       tCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCt                                                                                                                             <  2:1110953/140‑1 (MQ=255)

                tctACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGCCACAGCATCCGCGTTATGCTGACTTTCCg                                                                                                                    >  1:826684/1‑140 (MQ=255)

                 ctACGGCATTCCTAAGTAAACTTGCACCGAGTAACACTCGGGCGTTTAGGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCgg                                                                                                                   <  2:787102/140‑1 (MQ=255)

                 ctACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCgg                                                                                                                   <  2:5443/140‑1 (MQ=255)

                   aCGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCTTTCCTGGCACCGCATCAGCGCTATGCTGACTTTCCGGCa                                                                                                                 >  2:623701/1‑140 (MQ=255)

                   aCGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCa                                                                                                                 >  1:1160297/1‑140 (MQ=255)

                   aCGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGCCACCGCATCCGCGCTATGCTGACTTTCCGGCa                                                                                                                 <  2:1160297/140‑1 (MQ=255)

                     ggCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGc                                                                                                               >  2:837108/1‑140 (MQ=255)

                     ggCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGc                                                                                                               >  1:1131835/1‑140 (MQ=255)

                      gCATTCCTAAGCAAACTTGCACAGAAAAACTCTCTAGCGTTTACGAGCTTCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCg                                                                                                              <  2:710115/140‑1 (MQ=255)

                      gCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCg                                                                                                              <  2:43361/140‑1 (MQ=255)

                        aTTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGtt                                                                                                            <  1:36619/140‑1 (MQ=255)

                         ttCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTg                                                                                                           >  1:1114170/1‑140 (MQ=255)

                         ttCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTg                                                                                                           >  1:230940/1‑140 (MQ=255)

                           ccTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGAt                                                                                                         >  2:1194255/1‑140 (MQ=255)

                            cTAAGCAAGCTCGCACCGAAAAACTCTCTGTAGTTAACGGGCATCATAATCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGAtt                                                                                                        <  2:783356/140‑1 (MQ=255)

                            cTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGAtt                                                                                                        <  2:230940/140‑1 (MQ=255)

                            cTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGAtt                                                                                                        <  2:274789/140‑1 (MQ=255)

                                 cAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGa                                                                                                   <  2:249617/140‑1 (MQ=255)

                                    aCTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTa                                                                                                >  2:1305002/1‑140 (MQ=255)

                                    aCTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTa                                                                                                >  1:228891/1‑140 (MQ=255)

                                    aCTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTa                                                                                                >  2:888005/1‑140 (MQ=255)

                                      tCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTaca                                                                                              >  1:1682124/1‑140 (MQ=255)

                                      tCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCGTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTaca                                                                                              <  2:1682124/140‑1 (MQ=255)

                                           ccGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGAt                                                                                         >  1:7597/1‑140 (MQ=255)

                                                      tctGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCt                                                                              >  2:1426146/1‑140 (MQ=255)

                                                          gCGTTTACGAGCATCATTTTCTTCATCCTCCCGTGTTTCCGACGCGTGTCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCa                                                                          <  2:826684/140‑1 (MQ=255)

                                                          gCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCa                                                                          <  1:346875/140‑1 (MQ=255)

                                                              ttACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACCCCGATCTGGAAATTCTGGCAgaga                                                                      <  2:1131835/140‑1 (MQ=255)

                                                                     catcatATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGgaaga                                                               <  2:228891/140‑1 (MQ=255)

                                                                      atcatATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGgaagaa                                                              >  2:211186/1‑140 (MQ=255)

                                                                         atatTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAggg                                                           <  2:1114170/140‑1 (MQ=255)

                                                                          tatTCTCCATCCTCATGCGCTTATGACGCGTGGCTCTGATGATTCATTGCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAgggg                                                          <  2:628419/140‑1 (MQ=255)

                                                                          tatTCTCCATCCTCAAGCGCTTCTGCCGCGTGGCTTTTATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAgggg                                                          <  1:623701/140‑1 (MQ=255)

                                                                           atTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGa                                                         <  2:7597/140‑1 (MQ=255)

                                                                               tcCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGa                                                         >  2:1501468/1‑136 (MQ=255)

                                                                               tcCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGa                                                         <  1:1501468/136‑1 (MQ=255)

                                                                               tcCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGAGATTACACCGATTTGGCAATTCTGGAAGAGCCGGAAGAAGGGGATGCa                                                     >  2:670380/1‑140 (MQ=255)

                                                                                 cATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCata                                                   >  2:121895/1‑140 (MQ=255)

                                                                                  aTCCTCATGCGCTTCTGTCGCGTGGCTTTGAAGATCCATCCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCatat                                                  <  2:515538/140‑1 (MQ=255)

                                                                                   tCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATc                                                 >  2:168472/1‑140 (MQ=255)

                                                                                    ccTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGTAAATTCTGGCACAGGCGGAAGTAGGGGATGCATATCt                                                >  1:515538/1‑140 (MQ=255)

                                                                                    ccTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCt                                                >  2:1130246/1‑140 (MQ=255)

                                                                                    ccTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCAGGACATTCTGGCAGAGGCGGAAGAAGGGGATGCATATCt                                                >  2:672913/1‑140 (MQ=255)

                                                                                      tCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGt                                              >  2:837159/1‑140 (MQ=255)

                                                                                       cATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGtt                                             >  2:321476/1‑140 (MQ=255)

                                                                                       cATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACAACGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGtt                                             >  2:632115/1‑140 (MQ=255)

                                                                                        aTGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGttt                                            <  2:325081/140‑1 (MQ=255)

                                                                                        aTGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGttt                                            >  2:970224/1‑140 (MQ=255)

                                                                                        aTGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGttt                                            <  1:168472/140‑1 (MQ=255)

                                                                                         tGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGttt                                            <  1:339483/139‑1 (MQ=255)

                                                                                         tGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGttt                                            >  2:339483/1‑139 (MQ=255)

                                                                                         tGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTg                                           <  2:198943/140‑1 (MQ=255)

                                                                                         tGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTg                                           >  1:198943/1‑140 (MQ=255)

                                                                                          gcgcTTCTGACGGGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGc                                          <  2:1048235/140‑1 (MQ=255)

                                                                                          gcgcTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGc                                          <  1:229123/140‑1 (MQ=255)

                                                                                             cTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAg                                       >  1:181222/1‑140 (MQ=255)

                                                                                             cTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAg                                       >  2:347861/1‑140 (MQ=255)

                                                                                             cTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAg                                       >  2:75421/1‑140 (MQ=255)

                                                                                                        tGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGTCAGAGACGGAAGGAGGGGATGCATAGCTGTGTGCCAGTAAAGATAAgc                            >  2:438457/1‑140 (MQ=255)

                                                                                                        tGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAgc                            >  2:1185756/1‑140 (MQ=255)

                                                                                                        tGGCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAgc                            >  2:1381149/1‑140 (MQ=255)

                                                                                                          gCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGTGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAgcgc                          >  2:1661717/1‑140 (MQ=255)

                                                                                                          gCTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAgcgc                          <  2:181222/140‑1 (MQ=255)

                                                                                                           cTTTGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCa                         >  1:1293085/1‑140 (MQ=255)

                                                                                                            tttGATGATTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCAt                        <  1:1381149/140‑1 (MQ=255)

                                                                                                                 tgatTCATTCCTGGCTCCGCATCCGGGCTATGCTGACGTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCt                   <  1:670380/140‑1 (MQ=255)

                                                                                                                 tgatTCATTCCTGGCTCCGCATCCGCGCTTTGCTGACATTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCt                   <  1:438457/140‑1 (MQ=255)

                                                                                                                 tgatTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCt                   <  1:75421/140‑1 (MQ=255)

                                                                                                                 tgatTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCt                   <  2:1086133/140‑1 (MQ=255)

                                                                                                                  gatTCATTCCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCtt                  <  1:1305002/140‑1 (MQ=255)

                                                                                                                    ttcattcCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtg                >  2:1184858/1‑140 (MQ=255)

                                                                                                                      cattcCTGGCACCGCTTCCGCGCTAAGCTAACTTTCCTGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgtg              <  1:569042/140‑1 (MQ=255)

                                                                                                                      cattcCTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTtgtg              <  2:1374872/140‑1 (MQ=255)

                                                                                                                           cTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACggg         <  1:347861/140‑1 (MQ=255)

                                                                                                                           cTGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACggg         <  1:970224/140‑1 (MQ=255)

                                                                                                                            tGGCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGc        >  2:26615/1‑140 (MQ=255)

                                                                                                                             ggCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCAtt                       >  1:412007/1‑124 (MQ=255)

                                                                                                                             ggCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCAtt                       <  2:412007/124‑1 (MQ=255)

                                                                                                                             ggCACCGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGcc       <  1:26615/140‑1 (MQ=255)

                                                                                                                             gagacagCATCCGCGCTATGCTAACTTTCCGGCAGCGCTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGcc       <  1:556828/134‑1 (MQ=255)

                                                                                                                                 ccGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATcc   <  1:632115/140‑1 (MQ=255)

                                                                                                                                  cGCATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATccc  >  2:1414559/1‑140 (MQ=255)

                                                                                                                                    cATCCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATcc   <  1:1252449/137‑1 (MQ=255)

GCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCC  >  NC_000913/4214718‑4214987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A