Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A36 F60 I1 R1
|
15 |
27.9 |
999172 |
98.4% |
983185 |
132.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,144,239 |
A→C |
D82A (GAC→GCC) |
pflD → |
putative formate acetyltransferase 2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,144,239 | 0 | A | C | 100.0%
| 86.3
/ NA
| 24 | D82A (GAC→GCC) | pflD | putative formate acetyltransferase 2 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (10/14); total (10/14) |
ATATTGCGCCGGGCGAAAGCAACAGCGTATATCCTTGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGACCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGA > NC_000913/4144112‑4144364
|
atatTGCGCCGGGCGAAAGCAACAGCGTATATCCTTGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCtt > 2:71937/1‑133 (MQ=255)
ccGGGCGAAAGCAACAGCGTATATCCTTGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGgctg > 2:79884/1‑133 (MQ=255)
ggCGAAAGCAACAGCGTATATCCTTGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCACGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGgctg < 2:350930/130‑1 (MQ=255)
ggCGAAAGCAACAGCGTATATCCTTGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCACGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGgctg > 1:350930/1‑130 (MQ=255)
aGCAACAGCGTATATCCTTGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCTCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAagag < 2:119846/133‑1 (MQ=255)
cAGCGTATATCCTTGAACATGTTGCAATTTCGCTTCGTGATCAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGa < 2:185238/133‑1 (MQ=255)
tGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGAcg > 1:373990/1‑133 (MQ=255)
aaCATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGAcgcg > 2:301753/1‑133 (MQ=255)
gTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGTAAATGGCCCCTTACTGGCTGCTGAAAAAGCTGGATAAATTCCCGACGCGTctgt > 2:4394/1‑130 (MQ=255)
gTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGc > 1:287396/1‑133 (MQ=255)
gTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGc > 2:46469/1‑133 (MQ=255)
gATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGTATCCATTTCCGACGCGACCGCGGGACCGCttt > 2:139483/1‑133 (MQ=255)
gaagaaCTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCg < 1:123169/133‑1 (MQ=255)
agaaCTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGACGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCgaa < 1:325299/133‑1 (MQ=255)
aCTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCgaagaa > 1:288471/1‑133 (MQ=255)
tGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAagaaga < 2:61361/133‑1 (MQ=255)
tGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATaaa > 1:415807/1‑133 (MQ=255)
gTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTAt < 1:46469/133‑1 (MQ=255)
cGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGaa < 1:79884/133‑1 (MQ=255)
gcgcgcCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTc < 1:71937/133‑1 (MQ=255)
cgcgcCGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTcc > 2:82882/1‑133 (MQ=255)
ccGGGATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTa < 2:373990/133‑1 (MQ=255)
gggATTATGTCGCCGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACt < 1:82882/133‑1 (MQ=255)
ccGGAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGtt < 2:415807/133‑1 (MQ=255)
gAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGa < 1:338210/133‑1 (MQ=255)
gAAATGGCCCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGa < 1:301753/133‑1 (MQ=255)
|
ATATTGCGCCGGGCGAAAGCAACAGCGTATATCCTTGAACATGTTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAAATGGACCCTTACTGGCTGCTGAAAGAGCTGGATCAATTCCCGACGCGTCCGCAGGACCGCTTTGCTATCAGCGAAGAAGATAAACGTATCTACCGTGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGA > NC_000913/4144112‑4144364
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
