Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	139,245	C→A	G661C (GGT→TGT)	gcd ←	quinoprotein glucose dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	139,245	0	C	A	95.3%	143.7 / ‑1.8	43	G661C (GGT→TGT)	gcd	quinoprotein glucose dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (21/20); total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTT  >  NC_000913/139107‑139380

tatTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCa                                                                                                                                        >  1:561928/1‑140 (MQ=255)

       cGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTg                                                                                                                                 >  1:310070/1‑140 (MQ=255)

       cGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTg                                                                                                                                 >  2:257918/1‑140 (MQ=255)

          cACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTgaga                                                                                                                              >  1:509154/1‑140 (MQ=255)

          cACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTgaga                                                                                                                              >  2:511557/1‑140 (MQ=255)

            ccGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAgg                                                                                                                            <  1:386767/140‑1 (MQ=255)

              ggAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGaa                                                                                                                          <  1:117767/140‑1 (MQ=255)

                     acATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTg                                                                                                                   >  1:424832/2‑140 (MQ=255)

                         cGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCg                                                                                                               <  1:233913/140‑1 (MQ=255)

                         cGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCg                                                                                                               <  2:61797/140‑1 (MQ=255)

                         cGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCg                                                                                                               <  2:281401/140‑1 (MQ=255)

                           ggAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTg                                                                                                             <  2:509154/140‑1 (MQ=255)

                                ggCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACAcc                                                                                                        <  2:402218/140‑1 (MQ=255)

                                     aCTGTCCTGCGGCGTACCAATACGTTTCTTCCACAACACTTCAATAGGTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACg                                                                                                   <  2:285910/140‑1 (MQ=255)

                                      atGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACgg                                                                                                  <  2:561928/139‑1 (MQ=255)

                                       tGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAGCCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGt                                                                                                 <  2:383466/140‑1 (MQ=255)

                                       tGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGt                                                                                                 <  1:156267/140‑1 (MQ=255)

                                       tGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGt                                                                                                 <  1:124316/140‑1 (MQ=255)

                                            tGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACAcc                                                                                            >  2:129783/1‑140 (MQ=255)

                                                   tACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACtgt                                                                                     >  2:75645/1‑140 (MQ=255)

                                                   tACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACtgt                                                                                     >  2:270216/1‑140 (MQ=255)

                                                                cttcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCgg                                                                        >  1:131871/1‑140 (MQ=255)

                                                                  tcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGAt                                                                      >  1:12418/1‑140 (MQ=255)

                                                                      caccacTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                                                  >  2:63815/1‑140 (MQ=255)

                                                                           cTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTAccc                                                             <  2:466988/140‑1 (MQ=255)

                                                                             tCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGt                                                           <  2:310070/140‑1 (MQ=255)

                                                                                   gTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCttt                                                     >  2:501367/1‑140 (MQ=255)

                                                                                   gTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCttt                                                     >  2:137761/1‑140 (MQ=255)

                                                                                     tttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTgg                                                   <  1:148187/140‑1 (MQ=255)

                                                                                     tttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTgg                                                   <  1:531197/140‑1 (MQ=255)

                                                                                      ttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGc                                                  >  2:125148/1‑140 (MQ=255)

                                                                                      ttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGc                                                  >  1:29522/1‑140 (MQ=255)

                                                                                               aGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCgg                                         <  2:47008/140‑1 (MQ=255)

                                                                                                           taACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACCCCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCgg                             <  2:534824/140‑1 (MQ=255)

                                                                                                           taACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCgg                             <  1:501367/140‑1 (MQ=255)

                                                                                                           taACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCgg                             <  2:131871/140‑1 (MQ=255)

                                                                                                           taACCCCATGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCgg                             <  1:257918/140‑1 (MQ=255)

                                                                                                                  aTGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCa                      >  1:46901/1‑140 (MQ=255)

                                                                                                                  aTGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCa                      >  1:27374/1‑140 (MQ=255)

                                                                                                                   tGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTTCATCGGGTTGCCAg                     >  2:365309/1‑140 (MQ=255)

                                                                                                                   tGCTGGCTGTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAg                     >  2:371470/1‑140 (MQ=255)

                                                                                                                      tggctgTTTACATGGCAGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGAc                  <  1:125148/140‑1 (MQ=255)

                                                                                                                                      aGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGttt  >  2:437277/1‑140 (MQ=255)

                                                                                                                                      aGACAAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGGGCCTTTTGCATCGTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGttt  >  1:461263/1‑140 (MQ=255)

TATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCTCCATCGGGTTGCCAGGACCACGCGGGATCAGTTT  >  NC_000913/139107‑139380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A