Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	330,549	C→A	L363M (CTG→ATG)	betT →	choline:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	330,549	0	C	A	100.0%	85.0 / NA	25	L363M (CTG→ATG)	betT	choline:H(+) symporter
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/13); total (12/13)

ACTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGGCGCAGTATCCGGCGTTTAC  >  NC_000913/330415‑330682

aCTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTa                                                                                                                                  >  1:581210/1‑140 (MQ=255)

      cGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGctc                                                                                                                            >  2:503482/1‑140 (MQ=255)

      cGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGctc                                                                                                                            >  2:14127/1‑140 (MQ=255)

      cGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTAACGATGTTATGGctc                                                                                                                            >  1:560168/1‑140 (MQ=255)

            tcttctGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTAGGGCTCTCGgtg                                                                                                                      <  1:504625/140‑1 (MQ=255)

              ttctGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGtt                                                                                                                    <  2:451101/140‑1 (MQ=255)

                     cATGGAGGGTGGCATGGTCGCCGCTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTTGTGCTGGGCACGGTGATTATTCCGTTTACCTTCACGATGTTAGGGCTCTCGGTGTTCGGCAAt                                                                                                             >  1:408822/1‑140 (MQ=255)

                           gggtggCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAgcgcg                                                                                                       <  1:398719/140‑1 (MQ=255)

                                                tCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCAcggc                                                                                  >  2:578451/1‑140 (MQ=255)

                                                              gcgcGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGc                                                                    <  1:57934/140‑1 (MQ=255)

                                                                         gcgTGGGCGTACCATTCGCCAGTTCGTGCTGGGCAC‑‑TGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATg                                                       >  1:437409/1‑140 (MQ=255)

                                                                          cgTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGAt                                                        <  1:578451/140‑1 (MQ=255)

                                                                              ggCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTc                                                    <  2:216811/140‑1 (MQ=255)

                                                                              ggCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTc                                                    >  1:117242/1‑140 (MQ=255)

                                                                                        tCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAgc                                          >  1:430627/1‑140 (MQ=255)

                                                                                          gCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAgcgc                                        <  1:333960/140‑1 (MQ=255)

                                                                                           ccAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGcgcg                                       <  1:306463/140‑1 (MQ=255)

                                                                                            cAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCgg                                      <  1:278171/140‑1 (MQ=255)

                                                                                               ttCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCtt                                   >  1:501862/1‑140 (MQ=255)

                                                                                               ttCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCtt                                   >  2:222886/1‑140 (MQ=255)

                                                                                                      tGGGCACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGc                            >  1:432986/1‑140 (MQ=255)

                                                                                                          cACGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCctgc                        <  1:107093/140‑1 (MQ=255)

                                                                                                           aCGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCctgct                       <  1:162179/140‑1 (MQ=255)

                                                                                                            cGTTGATTATTCCGTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCctgctg                      <  2:363089/140‑1 (MQ=255)

                                                                                                                         gTTTACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCTCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGGCGCAGTATCCgg         <  1:189109/140‑1 (MQ=255)

                                                                                                                           ttACCTTCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCTGCTTCTACAGCCTGCTGGCTCAGTATCCGGct       >  2:238960/1‑139 (MQ=255)

                                                                                                                                ttCACGATGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGGCGCAGTATCCGGCGTTTAc  >  2:514234/1‑140 (MQ=255)

ACTGGACGCTCTTCTTCTGGGCATGGTGGGTGGCATGGTCGCCGTTTGTCGGCTTGTTCCTGGCGCGTATCTCGCGTGGGCGTACCATTCGCCAGTTCGTGCTGGGCACGTTGATTATTCCGTTTACCTTCACGCTGTTATGGCTCTCGGTGTTCGGCAATAGCGCGCTGTATGAAATCATCCACGGCGGCGCGGCATTTGCCGAGGAAGCGATGGTCCATCCGGAGCGCGGCTTCTACAGCCTGCTGGCGCAGTATCCGGCGTTTAC  >  NC_000913/330415‑330682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A