Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F48 I1 R1	66	101.5	3442112	98.9%	3404248	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	334,247	C→A	E63* (GAG→TAG)	yahB ←	putative LysR‑type DNA‑binding transcriptional regulator YahB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	334,247	0	C	A	100.0%	381.0 / NA	109	E63* (GAG→TAG)	yahB	putative LysR‑type DNA‑binding transcriptional regulator YahB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (54/55); total (54/55)

Only 100 of 109 total aligned reads displayed.

GGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGC  >  NC_000913/334111‑334383

ggCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGt                                                                                                                                       <  2:1598102/140‑1 (MQ=255)

  cGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTa                                                                                                                                     <  1:1701707/140‑1 (MQ=255)

   gTATAAAGCAGCTGATTAATACAAATGCG‑‑CACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACt                                                                                                                                  <  1:1385937/140‑1 (MQ=255)

    tataAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAAc                                                                                                                                   >  1:1366043/1‑140 (MQ=255)

       aaaGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCa                                                                                                                                <  2:954844/140‑1 (MQ=255)

       aaaGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCa                                                                                                                                <  2:448396/140‑1 (MQ=255)

              cTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAAtgctgc                                                                                                                         >  2:1261612/1‑140 (MQ=255)

               tGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCg                                                                                                                        <  2:861196/140‑1 (MQ=255)

               tGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCg                                                                                                                        >  2:1270650/1‑140 (MQ=255)

               tGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCg                                                                                                                        >  1:1602621/1‑140 (MQ=255)

               tGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCg                                                                                                                        >  2:1598499/1‑140 (MQ=255)

               tGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCg                                                                                                                        <  1:1371691/140‑1 (MQ=255)

                  ttAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGt                                                                                                                     >  2:596083/1‑140 (MQ=255)

                     aTACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACt                                                                                                                  >  2:1155898/1‑140 (MQ=255)

                         aaaTGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGa                                                                                                              <  1:646742/140‑1 (MQ=255)

                           aTGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGt                                                                                                            >  1:590842/1‑140 (MQ=255)

                           aTGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGt                                                                                                            >  2:441166/1‑140 (MQ=255)

                           aTGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGt                                                                                                            >  2:129527/1‑140 (MQ=255)

                               gcACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAAc                                                                                                        <  1:441166/140‑1 (MQ=255)

                                cacaCGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGa                                                                                                              >  1:817890/1‑133 (MQ=255)

                                cacaCGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGa                                                                                                              <  2:817890/133‑1 (MQ=255)

                                cacaCGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACa                                                                                                       >  2:356272/1‑140 (MQ=255)

                                cacaCGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACa                                                                                                       >  2:641577/1‑140 (MQ=255)

                                    cgcgCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCTTAAAAATCATTCAGCAGGTCGGTAGTTTTGCGGAATAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGcac                                                                                                   <  1:1651721/140‑1 (MQ=255)

                                     gcgcTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAGAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGcacc                                                                                                  <  2:1309225/140‑1 (MQ=255)

                                     gcgcTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGcacc                                                                                                  >  2:111946/1‑140 (MQ=255)

                                           cTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATcc                                                                                            >  1:1533911/1‑140 (MQ=255)

                                             aTGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAg                                                                                          >  2:82369/1‑140 (MQ=255)

                                                cccTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGccc                                                                                       <  1:1348634/140‑1 (MQ=255)

                                                cccTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGccc                                                                                       <  1:356272/140‑1 (MQ=255)

                                                 ccTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGcccc                                                                                      <  2:1366043/140‑1 (MQ=255)

                                                 ccTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGcccc                                                                                      <  1:104538/140‑1 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:971155/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    <  1:790656/140‑1 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:333604/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    <  1:82369/140‑1 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:415662/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  1:939006/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:107351/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:1096113/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:426173/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:559882/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  1:1665489/1‑140 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    <  1:1155898/140‑1 (MQ=255)

                                                   tGCGAAATAGTATCGATTCTGCGATTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGt                                                                                    >  2:15276/1‑140 (MQ=255)

                                                     cGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGtct                                                                                  >  2:662822/1‑140 (MQ=255)

                                                     cGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGtct                                                                                  >  2:1079743/1‑140 (MQ=255)

                                                     cGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGtct                                                                                  >  1:415854/1‑140 (MQ=255)

                                                     cGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGtcc                                                                                  >  1:466956/1‑139 (MQ=255)

                                                       aaaTAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTcc                                                                                <  1:1668686/140‑1 (MQ=255)

                                                        aaTAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCa                                                                               >  1:951886/1‑140 (MQ=255)

                                                         aTAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCAt                                                                              <  2:1412654/140‑1 (MQ=255)

                                                          tAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATa                                                                             >  1:1379729/1‑140 (MQ=255)

                                                           aGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATAc                                                                            <  1:1079743/140‑1 (MQ=255)

                                                            gTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACg                                                                           <  1:971155/140‑1 (MQ=255)

                                                              aTCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCt                                                                         <  1:989912/140‑1 (MQ=255)

                                                                cGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTa                                                                       <  2:1366110/140‑1 (MQ=255)

                                                                 gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTaa                                                                      <  2:951886/140‑1 (MQ=255)

                                                                 gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTaa                                                                      <  1:129527/140‑1 (MQ=255)

                                                                 gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTaa                                                                      <  1:1331763/140‑1 (MQ=255)

                                                                 gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTaa                                                                      <  1:1261612/140‑1 (MQ=255)

                                                                 gATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTaa                                                                      <  1:1270650/140‑1 (MQ=255)

                                                                  aTTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAAt                                                                     >  2:1442557/1‑140 (MQ=255)

                                                                   ttCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACCTCCAGCCCCGTCTCCATACGCTTAATg                                                                    <  1:1395985/140‑1 (MQ=255)

                                                                   ttCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATg                                                                    <  1:596083/140‑1 (MQ=255)

                                                                   ttCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATg                                                                    <  2:590842/140‑1 (MQ=255)

                                                                    tCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATgg                                                                   >  2:1666367/1‑140 (MQ=255)

                                                                     cTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGgt                                                                  >  2:277915/1‑140 (MQ=255)

                                                                      tGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGgtg                                                                 <  1:1096113/140‑1 (MQ=255)

                                                                       gCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGgtgt                                                                >  1:1689673/1‑140 (MQ=255)

                                                                       gCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGgtgt                                                                >  2:422587/1‑140 (MQ=255)

                                                                       gCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGgtgt                                                                >  1:1254458/1‑140 (MQ=255)

                                                                       gCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGgtgt                                                                >  1:1165425/1‑140 (MQ=255)

                                                                        cGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTa                                                               <  1:662822/140‑1 (MQ=255)

                                                                           ttGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCt                                                            <  2:1602621/140‑1 (MQ=255)

                                                                            tGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTa                                                           <  1:1038689/140‑1 (MQ=255)

                                                                             gATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTaa                                                          <  2:1665489/140‑1 (MQ=255)

                                                                             gATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTaa                                                          >  1:1329444/1‑140 (MQ=255)

                                                                                ggCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATgg                                                       <  2:424677/140‑1 (MQ=255)

                                                                                  cATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCg                                                     >  1:511835/1‑140 (MQ=255)

                                                                                    tAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGa                                                   >  2:1421917/1‑140 (MQ=255)

                                                                                     aaaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGaa                                                  <  2:1689673/140‑1 (MQ=255)

                                                                                     aaaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGaa                                                  <  1:333604/140‑1 (MQ=255)

                                                                                     aaaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGaa                                                  <  1:177459/140‑1 (MQ=255)

                                                                                     aaaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCACCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGaa                                                  <  1:1085700/140‑1 (MQ=255)

                                                                                      aaaaTCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAg                                                 <  1:641577/140‑1 (MQ=255)

                                                                                           cATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGtt                                            <  2:1165425/140‑1 (MQ=255)

                                                                                              tCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTaaa                                         <  1:1666367/140‑1 (MQ=255)

                                                                                              tCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTaaa                                         <  1:1598499/140‑1 (MQ=255)

                                                                                                  cagGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCa                                     >  1:551582/1‑140 (MQ=255)

                                                                                                           aGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAACCCCAACTCTTCTg                            <  2:1379729/140‑1 (MQ=255)

                                                                                                           aGCTTTGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTg                            <  1:277915/140‑1 (MQ=255)

                                                                                                               ttGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGCCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGc                        >  2:1079190/1‑140 (MQ=255)

                                                                                                                tGCGGAAAAAATAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCt                       >  1:1126941/1‑140 (MQ=255)

                                                                                                                       aaaaTAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGa                <  2:511835/140‑1 (MQ=255)

                                                                                                                       aaaaTAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGa                <  1:244388/140‑1 (MQ=255)

                                                                                                                       aaaaTAACGCCCGGACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGa                <  2:415854/140‑1 (MQ=255)

                                                                                                                                   ggACTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAc    >  1:899709/1‑140 (MQ=255)

                                                                                                                                     aCTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAcgc  <  1:267773/140‑1 (MQ=255)

                                                                                                                                     aCTACGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAAcgc  <  2:1554931/140‑1 (MQ=255)

GGCGTATAAAGCAGCTGATTAATACAAATGCGCACACGCGCTTCTATGCCCTGCGAAATAGTATCGATTCTGCGTTTGATGGCATAAAAATCATTCAGCAGGTCGGTAGCTTTGCGGAAAAAATAACGCCCGGACTCCGTTAACTCAATGCTGCGGGTACTGCGAGTGAACAGCACCACATCCAGCCCCGTCTCCATACGCTTAATGGTGTAGCTAATGGCGGAAGTGGTTAAACCCAACTCTTCTGCGGCTTTACTGAAGCTGCCAAAACGC  >  NC_000913/334111‑334383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A