Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	388,636	G→T	R281L (CGG→CTG)	tauD →	taurine dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	388,636	0	G	T	100.0%	83.4 / NA	25	R281L (CGG→CTG)	tauD	taurine dioxygenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (11/14); total (11/14)

GTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCT  >  NC_000913/388500‑388772

gTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATctggc                                                                                                                                       <  2:391670/140‑1 (MQ=255)

   cgctggcgctggCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATctggcggg                                                                                                                                    >  2:533441/1‑140 (MQ=255)

                aaCCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTCCCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGt                                                                                                                       <  1:81838/140‑1 (MQ=255)

                aaCCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGt                                                                                                                       <  2:406317/140‑1 (MQ=255)

                   cAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGa                                                                                                                    <  1:280645/140‑1 (MQ=255)

                          tatTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTccc                                                                                                             >  2:143296/1‑140 (MQ=255)

                              gCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCg                                                                                                         <  1:223063/140‑1 (MQ=255)

                                    tGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCt                                                                                                   >  2:589797/1‑140 (MQ=255)

                                          aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGga                                                                                             <  1:577889/140‑1 (MQ=255)

                                          aaCCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGga                                                                                             <  2:285167/140‑1 (MQ=255)

                                            acgcgTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGgaga                                                                                           <  2:89563/139‑1 (MQ=255)

                                                gTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGt                                                                                       <  1:388588/140‑1 (MQ=255)

                                                         cACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAg                                                                              <  2:258377/140‑1 (MQ=255)

                                                              tGCCAATGCCGATTACCTGCCACAGCGACGGCTAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGAGGACGGTTCTCTCGCCCCCTTGGTGAGTGGGGTACTGCGAGGGAGc                                                                         >  2:78240/1‑140 (MQ=255)

                                                              tGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGTACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAgggggg                                                                         >  1:202493/1‑139 (MQ=255)

                                                              tGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGc                                                                         >  2:179141/1‑140 (MQ=255)

                                                              tGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGc                                                                         >  1:97778/1‑140 (MQ=255)

                                                                     gCCGTTTACCTGCCACAGTGACGGATAGTGCATCGGGCGGCGATCCTTGGGGATAGACCGTTTTATCTGGCGGGGTAATCCGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCAcc                                                                  <  2:355182/140‑1 (MQ=38)

                                                                        gATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTa                                                               >  2:189998/1‑140 (MQ=255)

                                                                        gATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTa                                                               <  2:537956/140‑1 (MQ=255)

                                                                                       cGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTaa                                                >  2:551674/1‑140 (MQ=255)

                                                                                           ggATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTcc                                            <  2:321100/140‑1 (MQ=255)

                                                                                                   cATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCt                                    >  2:407669/1‑140 (MQ=255)

                                                                                                      cGGGCGACGATCCTTGGGGATAAACCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTgag                                 >  1:205808/1‑140 (MQ=255)

                                                                                                                           aaaCCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGa            <  1:189998/140‑1 (MQ=255)

                                                                                                                            aaCCGTTTTATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGaa           >  2:195299/1‑140 (MQ=255)

                                                                                                                                 ttttATCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtcttt      >  2:187933/1‑139 (MQ=255)

                                                                                                                                     aTCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtcttcttct  <  1:551674/140‑1 (MQ=255)

                                                                                                                                     aTCTGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAAtcttcttct  <  2:63349/140‑1 (MQ=255)

GTGCGCTGGCGCTGGCAACCAAATGATATTGCGATTTGGGATAACCGCGTGACCCAGCACTATGCCAATGCCGATTACCTGCCACAGCGACGGATAATGCATCGGGCGACGATCCTTGGGGATAAACCGTTTTATCGGGCGGGGTAATACGAGAGTGGACGGTCCCCTCGCCCCCTTGGGGAGAGGGTTAGGGTGAGGGGGCGTTCACCGTACTTTCAACAGGTTAACTCCCCCTTTCTGAGAGGAAACAAAATTAACGCAGAATCTTCTTCT  >  NC_000913/388500‑388772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A