Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F48 I1 R1
|
90 |
35.3 |
1199032 |
98.5% |
1181046 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
457,774 |
C→A |
L117M (CTG→ATG) |
clpX → |
ATP‑dependent Clp protease ATP‑binding subunit ClpX |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 457,774 | 0 | C | A | 96.5%
| 92.3
/ ‑5.4
| 29 | L117M (CTG→ATG) | clpX | ATP‑dependent Clp protease ATP‑binding subunit ClpX |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (17/11); total (17/12) |
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACAT > NC_000913/457649‑457907
|
aCGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGGATACAACCATTACAAACGGCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGAcc > 2:391844/1‑140 (MQ=255)
gATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGGCTCGGAATACAACCCTTACAAAAGTCTACGCCACGGCGATACCTGCAATTGCGTCGAGTTTGGCAAAAGAAAAAATCTGGTGATCGGTCCGAccct > 2:515874/1‑138 (MQ=255)
gTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCgg < 2:19924/140‑1 (MQ=255)
tATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTa < 1:277494/140‑1 (MQ=255)
gCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGa > 1:163794/1‑140 (MQ=255)
aaaaaaGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAAc > 2:156877/1‑140 (MQ=255)
aaaaaGTGCTGGCGGTCGCTGTATTCACCCATTACAAATGTCTGTGCCACGGTGGTACTAGCCCAGGTGTCGCGTTGGGCAAAAGTCACATTCTGGTGATCCGTTCGACCGGTTCCGGTTAAACTCTGCTTGCTGaaaat > 2:325334/1‑138 (MQ=255)
aaaaaGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACg > 2:98278/1‑140 (MQ=255)
aaaaaGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACg > 2:466093/1‑140 (MQ=255)
aaaaaGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACg > 1:300548/1‑140 (MQ=255)
tGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGgcg < 2:24861/140‑1 (MQ=255)
ggTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAGCATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCctgc < 1:325334/140‑1 (MQ=255)
tataCAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGtt < 1:156877/140‑1 (MQ=255)
tataCAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGtt < 2:163794/140‑1 (MQ=255)
ttACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCa > 1:65224/1‑140 (MQ=255)
aCAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATg > 2:43911/1‑140 (MQ=255)
cGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGTCTGCTGGATGTTCCGTTCACCATGGCCGa < 1:355425/140‑1 (MQ=255)
cGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGa > 2:84720/1‑140 (MQ=255)
aGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGc < 1:389898/140‑1 (MQ=255)
gCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGcc > 1:552231/1‑140 (MQ=255)
aaTGGCGTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCgg > 1:54166/1‑140 (MQ=255)
gTCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtgt > 1:320072/1‑140 (MQ=255)
tCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtgtg > 2:544844/1‑140 (MQ=255)
tCGAGTTGGGCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtgtg > 1:502915/1‑140 (MQ=255)
gggCAAAAGTAACATTTTGATGTTCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGTTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTggtttaat > 2:21936/1‑134 (MQ=255)
gggCAAAAGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAg > 2:430274/1‑140 (MQ=255)
aGTAACATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGa < 1:466093/140‑1 (MQ=255)
aCATTCTAATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAc > 1:523852/1‑140 (MQ=255)
cATTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAca < 1:98278/140‑1 (MQ=255)
aTTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAcat < 1:515874/140‑1 (MQ=255)
aTTCTGATGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAcat < 1:544844/140‑1 (MQ=255)
|
ACGATTACGTTATCGGCCAGGAACAGGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACAT > NC_000913/457649‑457907
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
