Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	487,967	G→T	A478S (GCG→TCG)	mscK →	potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	487,967	0	G	T	100.0%	37.3 / NA	12	A478S (GCG→TCG)	mscK	potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (8/4); total (8/4)

CCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGG‑CAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTG  >  NC_000913/487831‑488100

ccAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGt                                                                                                                                     >  1:212656/1‑140 (MQ=255)

ccAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGt                                                                                                                                     >  2:338963/1‑140 (MQ=255)

    cTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTccc                                                                                                                                 >  1:236689/1‑140 (MQ=255)

       atgatgGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCa                                                                                                                              <  1:425416/140‑1 (MQ=255)

              ccATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCCTg                                                                                                                       <  2:44667/140‑1 (MQ=255)

              acATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCCTg                                                                                                                       <  2:276936/139‑1 (MQ=255)

                   aaCCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCCTGAAAGa                                                                                                                  <  1:574142/140‑1 (MQ=255)

                                                         gAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGG‑Caaa                                                                           >  2:186004/1‑140 (MQ=255)

                                                                      accatccAGACTCAGAAATTCTTTTGGGTGAACAGTACCCGTCC‑AATGGACTGGGACTGGATCAAATCGTCCCCGAAAAGCCTGAAAGATGAATTAAAGTCGATAAAAATCCCGGTGAACTGC‑CAAAAAGCCGGGCccgc                                                              <  2:121108/139‑1 (MQ=255)

                                                                       ccatccTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGG‑CAAAAAGCCTGGCccgcc                                                             >  2:548961/1‑140 (MQ=255)

                                                                                ctaaGCAAATCTTTTGGGTGAAAAG‑AACCCTCCAAATGTACTGGGACGGAATAAATCCGTCCCCCAAAAGCCAGAAAGATGAATTTAAGTCGATAAAAATC‑GGGTTAACCGGAAAAAAACCCTGGCCCGCCGTTTTTATc                                                    <  2:556122/137‑1 (MQ=255)

                                                                                     cAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGG‑CAAAAAGCCTGGCCCGCCGTTTTTATCGCttt                                               >  2:424114/1‑140 (MQ=255)

                                                                                     aaaaTTTTTTGGGAAAACAATAACCGTCA‑AATGGACTGGGACTGGATAAAATCGTCCCCGAAAAGCGTAAAAGATGAATATAAGCCAAAGAAATTATGGGGGATCTGG‑CAAAAAGCCGGGCCCGCCGTTTTTATCGCttt                                               <  2:290157/139‑1 (MQ=255)

                                                                                        aTCTTTTGGGTGAACAGTACCCGTCA‑AATGGACGGGGACTGGATAAAATCGTCCCCGAAAAGCCTGAAAGATGAATTTAAGTGGATGAAAATCACGGTGAACGGG‑CAAAAGGCCTGGCCCGCCGTTTTTATCGCTTTCCt                                            <  2:174539/140‑1 (MQ=255)

                                                                                                     aaaGTAACCGTCA‑AATGGACGGGGATTGGAACAAATCGTTCCCGAAAAGCCTGAAAGATGAATTTAAGTCGATAAAAATCACGGTGAACTGG‑AAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCg                               <  2:182873/138‑1 (MQ=255)

                                                                                                              gTCC‑AATGGACTGGGACTGGATCAAATCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGAGAACTGG‑CAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTg                      >  1:306259/1‑140 (MQ=255)

                                                                                                                         gggggactggATAAAATCGTTCCCGAAACGCCTGAAAGATGAATTAAGGGCGATAAAAATCAGGGTGAACGGC‑AAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGc            <  2:212656/138‑1 (MQ=255)

                                                                                                                             gactggATCAAATCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGG‑CAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGAt        >  1:254796/1‑140 (MQ=255)

                                                                                                                                  aataaaaTCGTCCCCGAAAAGCCTAAAAGATGATTTTAAGTCGATGAAAATCACGGTGAACTGG‑AAAAAACCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACt   <  2:165121/136‑1 (MQ=255)

                                                                                                                                   aTCAAATCGTTCCCGCAAAGCCTGAAAGATAAATTTAAGTCGATAAAAATCACGGTAACCTGG‑AAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTg  <  1:424114/140‑1 (MQ=255)

CCAGCTGATGATGGCCATTAACCTGCAAATCAACCAGCAGCAGTTAATGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCC‑AATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCACGGTGAACTGG‑CAAAAAGCCTGGCCCGCCGTTTTTATCGCTTTCCTCGCTGGTTTGCCGCTGCTGTTGATTGCCGGGCTGATCCACTG  >  NC_000913/487831‑488100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A