Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	681,397	C→A	P396T (CCG→ACG)	djlC →	co‑chaperone DjlC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	681,397	0	C	A	100.0%	109.8 / NA	32	P396T (CCG→ACG)	djlC	co‑chaperone DjlC
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/16); total (16/16)

TATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGT  >  NC_000913/681267‑681521

tATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAag                                                                                                                     <  1:354622/140‑1 (MQ=255)

     aGTCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATg                                                                                                                >  2:597571/1‑140 (MQ=255)

     aGTCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATg                                                                                                                >  2:206268/1‑140 (MQ=255)

       tCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCt                                                                                                              >  1:95253/1‑140 (MQ=255)

          ttATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGc                                                                                                           <  1:45184/140‑1 (MQ=255)

           tATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGcc                                                                                                          <  1:487781/140‑1 (MQ=255)

           tATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGcc                                                                                                          <  1:574809/140‑1 (MQ=255)

                ttcAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCt                                                                                                     <  2:69427/140‑1 (MQ=255)

                ttcAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCt                                                                                                     <  2:208930/140‑1 (MQ=255)

                 tcAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACGGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCtt                                                                                                    >  2:465906/1‑140 (MQ=255)

                       gcAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACa                                                                                              <  1:235684/140‑1 (MQ=255)

                                           aaaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                          <  1:334784/140‑1 (MQ=255)

                                            aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCt                                                                         <  1:251678/140‑1 (MQ=255)

                                                  gagTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCa                                                                   >  1:524323/1‑140 (MQ=255)

                                                      cGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTag                                                               <  1:597571/140‑1 (MQ=255)

                                                      cGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCATAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTag                                                               <  1:136677/140‑1 (MQ=255)

                                                                        aGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGa                                             <  1:253179/140‑1 (MQ=255)

                                                                               acGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCtttt                                      <  2:202767/139‑1 (MQ=255)

                                                                                cGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATg                                           >  1:512620/1‑134 (MQ=255)

                                                                                cGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATg                                           <  2:512620/134‑1 (MQ=255)

                                                                                   tCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACg                                  >  1:568227/1‑140 (MQ=255)

                                                                                    ccTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGa                                 >  1:94388/1‑140 (MQ=255)

                                                                                    ccTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGa                                 >  2:422360/1‑140 (MQ=255)

                                                                                     cTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGaa                                >  2:61967/1‑140 (MQ=255)

                                                                                     cTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGaa                                >  2:597495/1‑140 (MQ=255)

                                                                                             gTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAAc                        >  1:319596/1‑140 (MQ=255)

                                                                                              tATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACt                       >  2:3860/1‑140 (MQ=255)

                                                                                                     tAGCGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCg                <  1:297274/140‑1 (MQ=255)

                                                                                                        cGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGcc                 <  2:467828/136‑1 (MQ=255)

                                                                                                        cGGAACAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGcc                 >  1:467828/1‑136 (MQ=255)

                                                                                                             cAGGTAGTACTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGa        >  1:204211/1‑140 (MQ=255)

                                                                                                                   gtaCTTGAAGCGTTTACGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGt  >  2:168950/1‑140 (MQ=255)

TATTGAGTCGTTATTCTTCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGT  >  NC_000913/681267‑681521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A