Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	744,706	C→T	S155L (TCG→TTG)	pxpC →	5‑oxoprolinase component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	744,706	0	C	T	100.0%	118.3 / NA	34	S155L (TCG→TTG)	pxpC	5‑oxoprolinase component C
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/13); total (21/13)

AGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGC  >  NC_000913/744573‑744837

aGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCg                                                                                                                               <  1:141078/140‑1 (MQ=255)

aGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCg                                                                                                                               <  2:411950/140‑1 (MQ=255)

   tATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGa                                                                                                                            <  1:35807/140‑1 (MQ=255)

     tCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGAtt                                                                                                                          <  1:25680/140‑1 (MQ=255)

          cGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTAtt                                                                                                                     >  2:20171/1‑139 (MQ=255)

            gTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGa                                                                                                                   >  2:272777/1‑140 (MQ=255)

              cgcgGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAACGTGGGGATTGGCGGGCTGGAAGGCCGCTTTCTGGAGGATGGTTTCCGACTCCCGATTTGAAAATTTAAGCCTGATTTTATGGaaa                                                                                                                 >  2:595659/1‑139 (MQ=255)

                               ttCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAg                                                                                                <  1:463726/140‑1 (MQ=255)

                                 ccgccgGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGGTTCTATGGAAGCGCAGGGCGTTAAACAgct                                                                                              >  1:445606/1‑140 (MQ=255)

                                 ccgccgGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgct                                                                                              >  1:268323/1‑140 (MQ=255)

                                 ccgccgGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgct                                                                                              >  2:535621/1‑140 (MQ=255)

                                 ccgccgGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAgct                                                                                              >  1:205626/1‑140 (MQ=255)

                                     cgGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGctgctg                                                                                          <  1:549389/140‑1 (MQ=255)

                                                 cATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGc                                                                              >  1:241007/1‑140 (MQ=255)

                                                  aTGCATCACCGATCTCAAAGTGGGGATTGGCGGGCTTGAAGGCCGTTTAATGAAGTATGGTGACCGACTCCCGATTGGCAAATTGAAGGGTGATTATATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCa                                                                             >  2:328539/1‑140 (MQ=255)

                                                  aTGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCa                                                                             >  2:428977/1‑140 (MQ=255)

                                                  aTGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCa                                                                             >  1:15692/1‑140 (MQ=255)

                                                                   aaGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCgggg                                                            <  2:259550/140‑1 (MQ=255)

                                                                    aGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGc                                                           >  2:380374/1‑140 (MQ=255)

                                                                    aGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGc                                                           <  1:535621/140‑1 (MQ=255)

                                                                        gggATTGGCGGGCTGGGAGGCCGTTTACTGAAGGGTGGTGAACGACTCCCGATTTGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGTGCAACCGCATTCGCGCCTTGTCGGGGCCGGa                                                       >  2:431317/1‑140 (MQ=255)

                                                                        gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGa                                                       >  2:132648/1‑140 (MQ=255)

                                                                        gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGa                                                       >  2:188285/1‑140 (MQ=255)

                                                                        gggATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGa                                                       <  2:531836/140‑1 (MQ=255)

                                                                                cGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATg                                               >  1:456012/1‑140 (MQ=255)

                                                                                             cGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCgcgc                                  >  1:433598/1‑140 (MQ=255)

                                                                                             cGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCgcgc                                  <  1:272777/140‑1 (MQ=255)

                                                                                                     gAAGGATGGTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATAGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAgg                          >  1:368608/1‑140 (MQ=255)

                                                                                                             gTGACCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTc                  >  2:92674/1‑140 (MQ=255)

                                                                                                                aCCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTgg               >  1:252437/1‑140 (MQ=255)

                                                                                                                aCCGACTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTgg               >  2:93751/1‑140 (MQ=255)

                                                                                                                    aCTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGtt           >  2:318289/1‑140 (MQ=255)

                                                                                                                     cTCCCGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTc          <  1:431317/140‑1 (MQ=255)

                                                                                                                       cccGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGc        <  2:252437/140‑1 (MQ=255)

                                                                                                                       cccGATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGc        >  1:264789/1‑140 (MQ=255)

                                                                                                                          gATTGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCt     <  2:205626/140‑1 (MQ=255)

                                                                                                                             tGGCAAATTGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGc  >  1:353975/1‑140 (MQ=255)

AGTTATCTGGCGGTCGCGGGTGGTATTGATGTTCCGCCGGTAATGGGCTCATGCAGCACCGATCTCAAAGTGGGGATTGGCGGGCTGGAAGGCCGTTTACTGAAGGATGGTGACCGACTCCCGATTGGCAAATCGAAGCGTGATTCTATGGAAGCGCAGGGCGTTAAACAGCTGCTGTGGGGCAACCGCATTCGCGCCTTGCCGGGGCCGGAATATCATGAGTTCGATCGCGCCTCGCAGGATGCATTCTGGCGTTCGCCCTGGC  >  NC_000913/744573‑744837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A