Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F48 I1 R1	90	35.3	1199032	98.5%	1181046	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,216	C→A	L104M (CTG→ATG)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,216	0	C	A	100.0%	124.5 / NA	37	L104M (CTG→ATG)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (27/10); total (27/10)

ATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACA  >  NC_000913/756088‑756348

aTGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGTATGCCGTTCTcg                                                                                                                           >  1:521549/1‑140 (MQ=255)

aTGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTcg                                                                                                                           >  2:86677/1‑140 (MQ=255)

aTGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTcg                                                                                                                           >  1:190294/1‑140 (MQ=255)

aTGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTcg                                                                                                                           >  2:556781/1‑140 (MQ=255)

aTGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTcg                                                                                                                           >  2:520729/1‑140 (MQ=255)

  gAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTcgcg                                                                                                                         >  1:286225/1‑140 (MQ=255)

        aaCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCga                                                                                                                   >  2:577063/1‑140 (MQ=255)

             ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGatgatg                                                                                                              <  1:562955/140‑1 (MQ=255)

               aaTGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGt                                                                                                            >  1:474474/1‑140 (MQ=255)

                aTGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTc                                                                                                           >  1:374692/1‑140 (MQ=255)

                            cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                                                                               >  1:390103/1‑140 (MQ=255)

                            cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAAc                                                                                               >  2:172036/1‑140 (MQ=255)

                               cacCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTc                                                                                            >  1:358595/1‑140 (MQ=255)

                                  cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                                                                                         >  1:113518/1‑140 (MQ=255)

                                  cGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGACCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt                                                                                         >  1:454617/1‑140 (MQ=255)

                                   gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGTATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGtt                                                                                        <  2:521549/140‑1 (MQ=255)

                                    tGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt                                                                                       >  1:419115/1‑140 (MQ=255)

                                           gTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTc                                                                                <  2:286225/140‑1 (MQ=255)

                                                     atCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGaaaaa                                                                      >  2:138890/1‑140 (MQ=255)

                                                        ggTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACtt                                                                   <  2:374692/140‑1 (MQ=255)

                                                            aCCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGTCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc                                                               >  1:285546/1‑140 (MQ=255)

                                                            aCCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggc                                                               >  2:68680/1‑140 (MQ=255)

                                                              cAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTcggcgg                                                             >  2:203964/1‑140 (MQ=255)

                                                                                 tgtgTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACt                                          <  1:405204/140‑1 (MQ=255)

                                                                                  gtgtAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTg                                         >  2:518984/1‑140 (MQ=255)

                                                                                  gtgtAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTg                                         >  1:208132/1‑140 (MQ=255)

                                                                                     tAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCgg                                      >  2:504432/1‑140 (MQ=255)

                                                                                       aaaCCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGgca                                    >  2:152976/1‑140 (MQ=255)

                                                                                            gggCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCagcagc                               >  1:284849/1‑140 (MQ=255)

                                                                                             ggCCGGAAGCGATTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCt                              >  2:457517/1‑140 (MQ=255)

                                                                                                        aTTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCgg                   >  1:319508/1‑140 (MQ=255)

                                                                                                        aTTCTGGAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGAACCgg                   <  2:390103/140‑1 (MQ=255)

                                                                                                             ggAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACg              <  2:190294/140‑1 (MQ=255)

                                                                                                             ggAACTCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACg              <  2:474474/140‑1 (MQ=255)

                                                                                                                  tCGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTg         <  2:113518/140‑1 (MQ=255)

                                                                                                                   cGAACACATGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGt        >  2:205843/1‑140 (MQ=255)

                                                                                                                         caTGGGCATGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGcaca  <  1:138890/140‑1 (MQ=255)

ATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCAGTCGAAAAACTTCGGCGGCGAGCAGGCGGCACGCACTGCGGCAGCAGCTGACCGTACCGGTCACGCACTGTTGCACA  >  NC_000913/756088‑756348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A