Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F48 I1 R1
|
90 |
35.3 |
1199032 |
98.5% |
1181046 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
779,696 |
C→A |
V33V (GTC→GTA) |
cpoB → |
cell division coordinator CpoB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 779,696 | 0 | C | A | 100.0%
| 61.1
/ NA
| 19 | V33V (GTC→GTA) | cpoB | cell division coordinator CpoB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (12/7); total (12/7) |
GTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGG > NC_000913/779566‑779832
|
gtcgtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGc > 1:529752/1‑140 (MQ=255)
cgtGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTaggctcaggctc > 1:124957/1‑140 (MQ=255)
cTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGa < 1:310331/140‑1 (MQ=255)
tGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGAc > 2:499336/1‑140 (MQ=255)
cTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTcc > 2:208031/1‑139 (MQ=255)
agaATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCaa > 1:203513/1‑140 (MQ=255)
agaATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCaa > 1:203529/1‑140 (MQ=255)
agaATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCaa > 1:403058/1‑140 (MQ=255)
cAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCAcag‑cca > 1:106780/1‑140 (MQ=255)
tCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAAccc < 2:106780/140‑1 (MQ=255)
tACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAg < 1:578647/140‑1 (MQ=255)
tataGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACttt > 2:142759/1‑140 (MQ=255)
cGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAAT‑CTTCCAGCCCACCTTTAAACCCAACTCCAGCACCACCTTTTTgtt > 2:19226/1‑138 (MQ=255)
ggCCCCCTGGGCCGCTTCTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATa > 2:579270/1‑140 (MQ=255)
cccccTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCGCGGTCGAAGACCGCGTCACTCAACGTGAGCGTATTTGTAATGCGCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTATCTGATaat < 2:545778/140‑1 (MQ=255)
cccccTGGCACGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATaat < 2:574325/140‑1 (MQ=255)
gggCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATcc < 2:124957/140‑1 (MQ=255)
gggCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATcc < 1:309700/140‑1 (MQ=255)
gggCCGCTTTTGCTCAGGCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATcc < 1:142759/140‑1 (MQ=255)
gCACCAATCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCg > 2:416626/1‑140 (MQ=255)
aaaTCAGTAGTGTAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGt < 2:17941/139‑1 (MQ=255)
tgtAGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAgg > 1:434160/1‑140 (MQ=255)
|
GTCGTGCGGTACTGGTTTACTAAGAGAATTGCATGAGCAGTAACTTCAGACATCAACTATTGAGTCTGTCGTTACTGGTTGGTATAGCGGCCCCCTGGGCCGCTTTTGCTCAGGCACCAATCAGTAGTGTCGGCTCAGGCTCGGTCGAAGACCGCGTCACTCAACTTGAGCGTATTTCTAATGCTCACAG‑CCAGCTTTTAACCCAACTCCAGCAACAACTTTCTGATAATCAATCCGATATTGATTCCCTGCGTGGTCAGATTCAGG > NC_000913/779566‑779832
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
