Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F48 I1 R1	66	101.5	3442112	98.9%	3404248	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,725	C→A	intergenic (+96/‑40)	lysT → / → valT	tRNA‑Lys/tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,725	0	C	A	100.0%	354.8 / NA	100	intergenic (+96/‑40)	lysT/valT	tRNA‑Lys/tRNA‑Val
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (32/68); total (32/68)

Only 100 of 106 total aligned reads displayed.

TTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTT  >  NC_000913/780588‑780859

ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcaac                                                                                                                                      >  2:652566/1‑140 (MQ=255)

ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcaac                                                                                                                                      <  2:33209/140‑1 (MQ=255)

    tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACt                                                                                                                                  <  1:1448288/140‑1 (MQ=255)

     cAATTGGTCGCAGGTTCGAATCATGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTa                                                                                                                                 <  1:1439694/140‑1 (MQ=255)

      aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTaa                                                                                                                                <  2:1517269/140‑1 (MQ=255)

       aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAAc                                                                                                                               <  1:17729/140‑1 (MQ=255)

       aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAAc                                                                                                                               <  1:392639/140‑1 (MQ=255)

       aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAAc                                                                                                                               <  1:948605/140‑1 (MQ=255)

        ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACa                                                                                                                              >  1:246634/1‑140 (MQ=255)

          ggTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAta                                                                                                                            <  2:1600856/140‑1 (MQ=255)

          ggTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAta                                                                                                                            <  2:373021/140‑1 (MQ=255)

          ggTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAta                                                                                                                            <  2:1543186/140‑1 (MQ=255)

          ggTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAta                                                                                                                            <  2:278246/140‑1 (MQ=255)

           gTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtag                                                                                                                           >  1:548389/1‑140 (MQ=255)

            tCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagt                                                                                                                          <  2:653031/140‑1 (MQ=255)

            tCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagt                                                                                                                          <  2:1046044/140‑1 (MQ=255)

            tCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagt                                                                                                                          <  1:178509/140‑1 (MQ=255)

             cGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagtt                                                                                                                         >  1:1518586/1‑140 (MQ=255)

               cAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagttag                                                                                                                       <  1:466491/140‑1 (MQ=255)

               cAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagttag                                                                                                                       <  1:227488/140‑1 (MQ=255)

               aaGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagttag                                                                                                                       <  2:640699/139‑1 (MQ=255)

               aaGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACAtagttag                                                                                                                       <  2:508644/139‑1 (MQ=255)

                 ggTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATagttagtt                                                                                                                     <  1:1519986/140‑1 (MQ=255)

                    tCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTgta                                                                                                                  <  2:355507/140‑1 (MQ=255)

                    tCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTgta                                                                                                                  <  2:230653/140‑1 (MQ=255)

                         tCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATc                                                                                                             <  1:333568/140‑1 (MQ=255)

                         tCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATc                                                                                                             <  1:1147249/140‑1 (MQ=255)

                         tCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATc                                                                                                             <  1:1192429/140‑1 (MQ=255)

                          ccTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATcc                                                                                                            <  1:594035/140‑1 (MQ=255)

                            tGCACGACCCACCAATCGCTAAGGTGGATGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAg                                                                                                          >  1:571859/1‑140 (MQ=255)

                             gCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGc                                                                                                         <  2:1210572/140‑1 (MQ=255)

                             gCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGc                                                                                                         <  2:316654/140‑1 (MQ=255)

                             gCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGc                                                                                                         <  2:1579123/140‑1 (MQ=255)

                             gCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGc                                                                                                         <  1:58206/140‑1 (MQ=255)

                             gCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGc                                                                                                         <  1:416954/140‑1 (MQ=255)

                              cACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCg                                                                                                        <  1:962679/140‑1 (MQ=255)

                              cACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCg                                                                                                        <  2:327998/140‑1 (MQ=255)

                              cACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCg                                                                                                        <  1:724921/140‑1 (MQ=255)

                              cACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCg                                                                                                        <  1:652566/140‑1 (MQ=255)

                                  aCCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCgtagt                                                                                                    >  1:1489531/1‑140 (MQ=255)

                                      accaATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCg                                                                                                >  2:60897/1‑140 (MQ=255)

                                      accaATCGCTAAGGAGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCg                                                                                                >  2:869744/1‑140 (MQ=255)

                                           tCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGa                                                                                           >  1:1514459/1‑140 (MQ=255)

                                             gCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGAtt                                                                                         >  1:1622464/1‑140 (MQ=255)

                                             gCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGAtt                                                                                         >  1:593634/1‑140 (MQ=255)

                                              cTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTa                                                                                        >  1:355979/1‑140 (MQ=255)

                                              cTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTa                                                                                        >  1:496067/1‑140 (MQ=255)

                                                aaGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGc                                                                                      <  2:498744/140‑1 (MQ=255)

                                                aaGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGc                                                                                      <  2:1167845/140‑1 (MQ=255)

                                                 aGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCt                                                                                     <  1:878093/140‑1 (MQ=255)

                                                     ggAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGc                                                                                 <  2:246634/140‑1 (MQ=255)

                                                     ggAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCACGCAGGGCGAGACGAAGTGGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGAGTAGTATCGGGTGATTAGCTCAGc                                                                                 <  2:1597171/140‑1 (MQ=37)

                                                        aGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTgg                                                                              >  1:632405/1‑140 (MQ=255)

                                                              agtaAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGc                                                                        <  2:1511089/140‑1 (MQ=255)

                                                                taAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                                                                      <  2:1514459/140‑1 (MQ=255)

                                                                taAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                                                                      <  2:1489531/140‑1 (MQ=255)

                                                                taAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                                                                      <  2:1195287/140‑1 (MQ=255)

                                                                 aaaaCGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAcc                                                                     <  2:870857/140‑1 (MQ=255)

                                                                  aaaCGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCt                                                                    <  1:22012/140‑1 (MQ=255)

                                                                  aaaCGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCt                                                                    <  2:571859/140‑1 (MQ=255)

                                                                    aCGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTcc                                                                  <  2:81003/140‑1 (MQ=255)

                                                                     cGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                                                                      <  1:223875/135‑1 (MQ=255)

                                                                     cGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCAc                                                                      >  2:223875/1‑135 (MQ=255)

                                                                       tGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCtt                                                               >  1:1403968/1‑140 (MQ=255)

                                                                                aCGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGGGATTAGCTTAGTTGGGAGAGCACCTCCCTTACAaggagg                                                      >  1:1302128/1‑140 (MQ=255)

                                                                                      cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                                >  1:933105/1‑140 (MQ=255)

                                                                                      cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                                >  2:1429255/1‑140 (MQ=255)

                                                                                      cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                                >  1:1241575/1‑140 (MQ=255)

                                                                                      cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                                >  1:1196608/1‑140 (MQ=255)

                                                                                      cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                                >  1:116976/1‑140 (MQ=255)

                                                                                      cATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcg                                                >  2:16297/1‑140 (MQ=255)

                                                                                       aTGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTcgg                                               <  2:1388063/140‑1 (MQ=255)

                                                                                           gCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGt                                           >  1:1121915/1‑140 (MQ=255)

                                                                                            cAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGtt                                          <  1:1586465/140‑1 (MQ=255)

                                                                                               cGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGa                                       >  2:1192986/1‑140 (MQ=255)

                                                                                                 gCCCGAAGGGCGAGACGAAGTCGAGACATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATc                                     <  1:825638/140‑1 (MQ=255)

                                                                                                  cccGAAGGGCGTGACGAAGTCGAGTCATCCTGCACGTCCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGATCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                                    <  1:1620140/140‑1 (MQ=255)

                                                                                                  cccGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                                    <  1:60897/140‑1 (MQ=255)

                                                                                                  cccGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                                    >  2:400302/1‑140 (MQ=255)

                                                                                                        gggCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                              <  2:1121915/140‑1 (MQ=255)

                                                                                                        gggCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                              <  2:1087382/140‑1 (MQ=255)

                                                                                                        gggCGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGtcat                              <  1:1192986/140‑1 (MQ=255)

                                                                                                           cGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcac                           >  1:361488/1‑140 (MQ=255)

                                                                                                           cGAGACGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcac                           >  2:1665076/1‑140 (MQ=255)

                                                                                                            gagaCGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcacc                          <  1:518564/140‑1 (MQ=255)

                                                                                                             agaCGAAGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccc                         >  1:1686512/1‑140 (MQ=255)

                                                                                                                   aGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCaccacc                   <  1:1350468/140‑1 (MQ=255)

                                                                                                                   aGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCaccacc                   <  2:593634/140‑1 (MQ=255)

                                                                                                                   aGTCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCaccacc                   >  2:594259/1‑140 (MQ=255)

                                                                                                                     tCGAGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCgg                 <  2:548389/140‑1 (MQ=255)

                                                                                                                        aGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTc              >  1:127563/1‑140 (MQ=255)

                                                                                                                        aGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTc              <  1:1103997/140‑1 (MQ=255)

                                                                                                                        aGTCATCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTc              >  2:962222/1‑140 (MQ=255)

                                                                                                                             tCCTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAg         >  1:397622/1‑140 (MQ=255)

                                                                                                                              ccTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGc        <  2:361488/140‑1 (MQ=255)

                                                                                                                              ccTGCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTAATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGc        <  2:1038389/140‑1 (MQ=255)

                                                                                                                                 gCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa     <  2:632405/140‑1 (MQ=255)

                                                                                                                                 gCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa     <  1:1506170/140‑1 (MQ=255)

                                                                                                                                 gCACGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCa     <  1:869744/140‑1 (MQ=255)

                                                                                                                                    cGACCAACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGtt  <  2:933105/140‑1 (MQ=255)

TTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACCGGGTCGTTAGCTCAGTT  >  NC_000913/780588‑780859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A