Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F48 I1 R1
|
90 |
35.3 |
1199032 |
98.5% |
1181046 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
828,800 |
C→A |
S61I (AGC→ATC) |
ybhF ← |
putative ABC exporter ATP binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 828,800 | 0 | C | A | 100.0%
| 73.1
/ NA
| 23 | S61I (AGC→ATC) | ybhF | putative ABC exporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/9); total (14/9) |
ATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCC > NC_000913/828676‑828934
|
aTTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTa > 1:461779/1‑140 (MQ=255)
tcCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCaa > 1:118119/1‑140 (MQ=255)
gaTCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGc < 1:467927/140‑1 (MQ=255)
gaTCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGc < 2:259096/140‑1 (MQ=255)
aaTCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGc < 2:461779/139‑1 (MQ=255)
tCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt < 1:443056/140‑1 (MQ=255)
tCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGt > 2:222119/1‑140 (MQ=255)
aGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTAcc > 2:166481/1‑140 (MQ=255)
aCCAAATTTCTGTGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTg > 2:137092/1‑140 (MQ=255)
aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAATCCAATCACCGTGGCACTGCCGATGTCGGGGTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTg > 2:236422/1‑140 (MQ=255)
aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTAcatt > 2:170497/1‑137 (MQ=255)
aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTg > 1:21296/1‑140 (MQ=255)
aCCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTg > 2:460734/1‑140 (MQ=255)
tGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCgg > 2:414597/1‑140 (MQ=255)
ggCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGccc > 2:261296/1‑140 (MQ=255)
cGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAAcccc < 2:118119/140‑1 (MQ=255)
aGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCACAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGt > 1:490827/1‑140 (MQ=255)
aGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGt > 1:179354/1‑140 (MQ=255)
cGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATaa < 1:137092/140‑1 (MQ=255)
cGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATaa > 1:346101/1‑140 (MQ=255)
gcgcCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTg < 1:222119/140‑1 (MQ=255)
cccGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGACCCACCAACCCCGTCACATAACCGGCGTGaa < 1:170497/139‑1 (MQ=255)
aGCCAATCACCGTGGCACTGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGAcg < 1:166481/140‑1 (MQ=255)
tGCCGATGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGgcc > 2:56191/1‑140 (MQ=255)
|
ATTGAGGTTCTCCATCACCGTGAGATCTTCATACAGACCAAATTTCTGCGGCATATAACCGAGCACGGCGTGCAGCGCGCCGTCGTTTTTGATCGGATCAAAGCCAATCACCGTGGCACTGCCGCTGTCGGGTTTCAGTAATCCCGCCAACATCCGCATCAGCGTGGTTTTACCTGCACCGTCCGGCCCCACCAACCCCGTCACATAACCGGCGTGAATGGTACAATCGAGCGGCGCGACGGCGGGCTTGTCCATGCCC > NC_000913/828676‑828934
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
