Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F48 I1 R1
|
90 |
35.3 |
1199032 |
98.5% |
1181046 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
906,254 |
C→A |
R167L (CGC→CTC) |
ybjS ← |
putative NAD(P)H‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 906,254 | 0 | C | A | 100.0%
| 98.1
/ NA
| 29 | R167L (CGC→CTC) | ybjS | putative NAD(P)H‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/13); total (16/13) |
CACGGCATTTTCATAGTAGGTCATATCGACCAGCGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCTTCTTTAATATCGCGA > NC_000913/906118‑906382
|
cACGGCATTTTCATAGTAGGTCATATCGACCAGCGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCa < 1:136868/140‑1 (MQ=255)
gtagGTCATATCGACCAGCGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAgcgct > 2:72168/1‑139 (MQ=255)
ggTCATATCGACCAGTGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGttt > 2:369238/1‑140 (MQ=255)
ggTCATATCGACCAGCGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGttt < 2:313386/140‑1 (MQ=255)
gTCATATCGACCAGCGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTtg > 1:132783/1‑140 (MQ=255)
gTCATATCGACCAGCGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTtg > 1:377805/1‑140 (MQ=255)
tGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGc < 2:550629/140‑1 (MQ=255)
gccgccATGCGGTATCAGAATACTGCCGTAGAGGTGCATCATATGCGCCAGACGGGAAATAAAGACTTTATCGCGCGTTCCTAACAGTCTTTTTGGGTGAAGAATAGCAAAGCGCGTTTGTGGATTCGCCTGCTAAAGCa > 2:516118/1‑140 (MQ=255)
ccATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATAtt > 1:48421/1‑140 (MQ=255)
ccATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATAtt > 2:158892/1‑140 (MQ=255)
tGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGAt < 1:83236/140‑1 (MQ=255)
aCAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCActtct < 2:135941/140‑1 (MQ=255)
tACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCgctg > 2:313806/1‑140 (MQ=255)
aCTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCgctgg < 2:377805/140‑1 (MQ=255)
tGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCgctggct > 1:379729/1‑140 (MQ=255)
gtggtgCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTtgc < 2:204613/140‑1 (MQ=255)
gtggtgCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTtgc > 1:329230/1‑140 (MQ=255)
tggtgCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTtgct > 1:527209/1‑140 (MQ=255)
tatGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAAc > 1:62295/1‑140 (MQ=255)
aCGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGGTTGACGATTCGACTTCTAAAGCATATTGATCACTTCTTCGCTTTCTGCTTTCCTTCGGGCAAACTTGTTGGctg > 2:468391/1‑138 (MQ=255)
tAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGa < 1:72168/140‑1 (MQ=255)
aGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAggg < 1:313806/140‑1 (MQ=255)
cTTTATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGa < 2:379729/140‑1 (MQ=255)
ttATCGTGCGGTCCGTACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGaaa > 1:319783/1‑140 (MQ=255)
ttATCGTGCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGaaa > 2:317815/1‑140 (MQ=255)
gCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAATGCATATTGATCACTTCTTCGCTGGCTGCTTCGCTGCGCGCAAACTCGTTGGCGAAGCGGTGAGGACGAAAAtcttct > 2:49156/1‑140 (MQ=255)
gCGGTCCGAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAAtcttct > 2:196857/1‑140 (MQ=255)
gAACAGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCTTCTTTAatat < 2:62295/140‑1 (MQ=255)
aGACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCTTCTTTAATATcgcg < 2:545382/140‑1 (MQ=255)
gACTTTGTGGGAGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCTTCTTTAATATCGCGa < 1:558360/140‑1 (MQ=255)
|
CACGGCATTTTCATAGTAGGTCATATCGACCAGCGCACTGCCGCCATGCGGTAACAGAATACTGCCGTAGTGGTGCATCATATGCGCCAGACGGGGAATAAAGACTTTATCGTGCGGTCCGAACAGACTTTGTGGGCGCAGAATAGTAAAGCGCGTTTGTGGATTCGCCTGCGAAAGCATATTGATCACTTCTTCGCTGGCTGCTTTGCTGCGGGCAAACTCGTTGGCGAAGCGGTGAGGGCGAAAATCTTCTTTAATATCGCGA > NC_000913/906118‑906382
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
