Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A17 F48 I1 R1
|
90 |
35.3 |
1199032 |
98.5% |
1181046 |
139.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
980,755 |
G→T |
G1477V (GGA→GTA) |
mukB → |
chromosome partitioning protein MukB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 980,755 | 0 | G | T | 100.0%
| 83.3
/ NA
| 25 | G1477V (GGA→GTA) | mukB | chromosome partitioning protein MukB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/8); total (17/8) |
AGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATT > NC_000913/980624‑980890
|
aGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACg > 1:1760/1‑140 (MQ=255)
aGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACg > 2:492076/1‑140 (MQ=255)
gcgcCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGa < 2:235357/140‑1 (MQ=255)
cAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAgg < 1:377573/140‑1 (MQ=255)
ccaccTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggc > 2:111218/1‑140 (MQ=255)
caccTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGTGCCGCAACTCCCTGAAATGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAgccg > 2:6996/1‑140 (MQ=255)
caccTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggct > 2:102886/1‑139 (MQ=255)
ccTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggcggc > 1:163534/1‑140 (MQ=255)
taAACTGGTGCGTAAAGTCTTCCAGAAGACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGgcagca > 1:486626/1‑140 (MQ=255)
aCTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATg < 2:1760/140‑1 (MQ=255)
ggTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggc > 1:449384/1‑127 (MQ=255)
ggTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCAGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggc < 2:449384/127‑1 (MQ=255)
gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgc > 1:67471/1‑140 (MQ=255)
gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgc > 1:100951/1‑140 (MQ=255)
gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgc > 2:27099/1‑140 (MQ=255)
gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgc > 2:332432/1‑140 (MQ=255)
gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgc > 2:42109/1‑140 (MQ=255)
gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgc > 1:230011/1‑140 (MQ=255)
gTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATgccgc > 2:76477/1‑140 (MQ=255)
cGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATCCCGCctt > 2:473960/1‑140 (MQ=255)
tACCGAACACGTTCATGTCGTCGGCCGGCGAGGATTTCCGCCCCAGGGCTCATATGAGTGACGGGTAACAGGGGAAGCTCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCCCCTTTTCTTTTTCGGAAAACt > 2:399267/1‑140 (MQ=255)
cgtcgGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTaaaaa < 1:311899/140‑1 (MQ=255)
tcgGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAg < 2:100951/140‑1 (MQ=255)
ggCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTg < 2:372410/140‑1 (MQ=255)
gcgcCGCAACTCCCTGAAACGCTTCCAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGgcgcg > 2:575216/1‑140 (MQ=255)
ccAGTAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACAtt < 1:42109/140‑1 (MQ=255)
|
AGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGCAATGCCGCCTTTTCTTTTTCCGAAAACTCCGTTTCTGCACTAAAAAAGTGGCACATTACGGCGCGCTTTTCTTTAAACTTCTTTACATT > NC_000913/980624‑980890
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
