Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F48 I1 R1	75	141.5	4784044	99.1%	4740987	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	620,013	G→T	H63N (CAT→AAT)	fepC ←	ferric enterobactin ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	620,013	0	G	T	99.3%	537.8 / ‑3.2	150	H63N (CAT→AAT)	fepC	ferric enterobactin ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (68/81); total (68/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 150 total aligned reads displayed.

GGATAACGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGCCA  >  NC_000913/619877‑620149

ggATAACGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTccc                                                                                                                                       <  1:2164419/140‑1 (MQ=255)

ggATAACGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCATAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTccc                                                                                                                                       >  1:1151734/1‑140 (MQ=255)

 gATAACGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCa                                                                                                                                      >  2:80477/1‑140 (MQ=255)

  aTAACGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCAt                                                                                                                                     >  2:1356371/1‑140 (MQ=255)

  aTAACGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCAt                                                                                                                                     >  2:1762865/1‑140 (MQ=255)

     aCGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAg                                                                                                                                  <  2:1017045/140‑1 (MQ=255)

            cgcgCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGt                                                                                                                           >  1:1107463/1‑140 (MQ=255)

            cgcgCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGt                                                                                                                           >  2:585190/1‑140 (MQ=255)

                  accaGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAg                                                                                                                     <  2:1449015/140‑1 (MQ=255)

                  accaGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAg                                                                                                                     <  1:2128882/140‑1 (MQ=255)

                  accaGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAg                                                                                                                     >  2:1602632/1‑140 (MQ=255)

                  accaGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAg                                                                                                                     <  1:1602632/140‑1 (MQ=255)

                  accaGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAg                                                                                                                     <  1:1370730/140‑1 (MQ=255)

                  accaGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAAGCCGGCGTGCAGCTTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAg                                                                                                                     <  1:814924/140‑1 (MQ=255)

                   acaGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAgg                                                                                                                    <  2:2086564/139‑1 (MQ=255)

                          cTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTa                                                                                                             <  1:1725890/140‑1 (MQ=255)

                          cTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTa                                                                                                             <  1:1927697/140‑1 (MQ=255)

                           tGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTaa                                                                                                            >  2:325994/1‑140 (MQ=255)

                           tGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTaa                                                                                                            >  2:950797/1‑140 (MQ=255)

                             cACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAgg                                                                                                          >  1:2341669/1‑140 (MQ=255)

                                  tGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGc                                                                                                     >  2:1604421/1‑140 (MQ=255)

                                  tGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGc                                                                                                     >  2:1608701/1‑140 (MQ=255)

                                  tGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGc                                                                                                     >  1:428379/1‑140 (MQ=255)

                                       tCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTaa                                                                                                <  1:1747386/140‑1 (MQ=255)

                                          cccGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGt                                                                                             >  1:1883882/1‑140 (MQ=255)

                                            cGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTgg                                                                                           >  2:1125761/1‑140 (MQ=255)

                                              gCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGAt                                                                                         >  1:1636460/1‑140 (MQ=255)

                                              gCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGAt                                                                                         >  1:2042589/1‑140 (MQ=255)

                                              gCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGAt                                                                                         >  2:2095751/1‑140 (MQ=255)

                                              gCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGAt                                                                                         >  2:2321616/1‑140 (MQ=255)

                                              gCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGAt                                                                                         >  2:62190/1‑140 (MQ=255)

                                                  ggTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTAc                                                                                     <  2:1089258/140‑1 (MQ=255)

                                                   gTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTAcc                                                                                    <  2:2307160/140‑1 (MQ=255)

                                                    tAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCg                                                                                   >  1:2390060/1‑140 (MQ=255)

                                                      gCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCa                                                                                 <  1:766477/140‑1 (MQ=255)

                                                      gCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCa                                                                                 <  1:1459381/140‑1 (MQ=255)

                                                         ttttGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGcc                                                                              >  1:974987/1‑140 (MQ=255)

                                                            tGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCCTCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCAtt                                                                           >  2:15963/1‑140 (MQ=255)

                                                            tGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCAtt                                                                           >  1:961379/1‑140 (MQ=255)

                                                            tGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCAtt                                                                           >  2:1942626/1‑140 (MQ=255)

                                                            tGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCAtt                                                                           >  1:10093/1‑140 (MQ=255)

                                                              cgcCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTg                                                                         >  1:2270820/1‑140 (MQ=255)

                                                                ccAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGc                                                                       >  2:173895/1‑140 (MQ=255)

                                                                  aaCAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGccc                                                                     >  1:2169588/1‑140 (MQ=255)

                                                                   aCAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCg                                                                    >  2:1586535/1‑140 (MQ=255)

                                                                     aGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGAt                                                                  <  2:431746/140‑1 (MQ=255)

                                                                       aCCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATaa                                                                <  1:276024/140‑1 (MQ=255)

                                                                           aTCCGGCGTGCAACTTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGc                                                            >  2:2208150/1‑140 (MQ=255)

                                                                            tgcGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCCGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTATCGTGGATTTACCGCAGCCATTTGGCCCGATAATTGcc                                                           <  1:250055/138‑1 (MQ=255)

                                                                            tCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGcc                                                           <  1:62190/140‑1 (MQ=255)

                                                                            tCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGcc                                                           <  1:1797044/140‑1 (MQ=255)

                                                                            tCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGcc                                                           <  1:1947240/140‑1 (MQ=255)

                                                                            tCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGcc                                                           >  1:985926/1‑140 (MQ=255)

                                                                            tCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGcc                                                           <  1:437962/140‑1 (MQ=255)

                                                                            tCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGcc                                                           <  1:750993/140‑1 (MQ=255)

                                                                               ggCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTg                                                        >  1:1660431/1‑140 (MQ=255)

                                                                                 cGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGaa                                                      >  2:1367242/1‑140 (MQ=255)

                                                                                   tGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGt                                                    <  2:1107463/140‑1 (MQ=255)

                                                                                   tGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGt                                                    <  2:2390060/140‑1 (MQ=255)

                                                                                   tGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGt                                                    <  2:428379/140‑1 (MQ=255)

                                                                                   tGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGt                                                    <  2:2169588/140‑1 (MQ=255)

                                                                                     cAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGa                                                  >  2:1875467/1‑140 (MQ=255)

                                                                                     cAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGa                                                  >  2:699230/1‑140 (MQ=255)

                                                                                     aaaCCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGa                                                  <  2:1490150/139‑1 (MQ=255)

                                                                                        ccTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCa                                               <  1:1942626/140‑1 (MQ=255)

                                                                                        ccTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCa                                               <  2:759213/140‑1 (MQ=255)

                                                                                        ccTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCa                                               <  1:1628050/140‑1 (MQ=255)

                                                                                        ccTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCa                                               <  2:1286845/140‑1 (MQ=255)

                                                                                         ctctTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCAt                                              <  2:961379/140‑1 (MQ=255)

                                                                                         ctctTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCAt                                              <  2:1710346/140‑1 (MQ=255)

                                                                                         ctctTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCAt                                              <  1:214919/140‑1 (MQ=255)

                                                                                           ctTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCa                                            <  1:1604421/140‑1 (MQ=255)

                                                                                           ctTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCa                                            <  1:950797/140‑1 (MQ=255)

                                                                                             ttACTGGCGTAATGTTGAATGTGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCa                                            <  2:2134448/140‑1 (MQ=255)

                                                                                             ttACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAgt                                          >  2:721371/1‑139 (MQ=255)

                                                                                             ttACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAgg                                          <  1:585190/140‑1 (MQ=255)

                                                                                             ttACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAgg                                          <  1:325994/140‑1 (MQ=255)

                                                                                                  ggCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTAttt                                     <  2:10093/140‑1 (MQ=255)

                                                                                                   gCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTc                                    >  1:1148027/1‑140 (MQ=255)

                                                                                                    cGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCt                                   >  1:827711/1‑140 (MQ=255)

                                                                                                      tAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTAc                                 >  1:1435351/1‑140 (MQ=255)

                                                                                                         tGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGt                              <  2:1542866/140‑1 (MQ=255)

                                                                                                             gAA‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGa                          >  2:569722/1‑140 (MQ=255)

                                                                                                              aa‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGAt                         >  2:1832417/1‑140 (MQ=255)

                                                                                                               a‑‑TGTGCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGAtt                        <  2:1660431/140‑1 (MQ=255)

                                                                                                                  tgtgCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGAttt                       >  1:2080510/1‑140 (MQ=255)

                                                                                                                     gCTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATTAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTcc                    >  1:1682162/1‑140 (MQ=255)

                                                                                                                      cTCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCg                   <  1:2321616/140‑1 (MQ=255)

                                                                                                                       tCGCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGc                  <  2:1636460/140‑1 (MQ=255)

                                                                                                                         gCCATCCAGCCAGACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCaa                <  2:1889437/140‑1 (MQ=255)

                                                                                                                              ccagccagACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGta           >  1:539846/1‑140 (MQ=255)

                                                                                                                              ccagccagACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGta           >  1:1323554/1‑140 (MQ=255)

                                                                                                                                 accagACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTatat        <  2:2009001/139‑1 (MQ=255)

                                                                                                                                 accagACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTatat        <  2:701535/139‑1 (MQ=255)

                                                                                                                                     gACATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGc    >  2:195023/1‑140 (MQ=255)

                                                                                                                                      aCATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGcc   <  2:2183376/140‑1 (MQ=255)

                                                                                                                                      aCATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGcc   <  2:1540926/140‑1 (MQ=255)

                                                                                                                                       cATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGCCa  <  1:520080/140‑1 (MQ=255)

                                                                                                                                       cATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGCCa  <  1:2283392/140‑1 (MQ=255)

                                                                                                                                       cATTCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGCCa  <  1:1832417/140‑1 (MQ=255)

GGATAACGTCCACGCGCCACCAGCTCCTGCACGGTGATATCGCCCGGCGTGGTAGCATTTTGCGCCAACAGACCAATCCGGCGTGCAACCTCTTTACTGGCGTAATGTTGAA‑‑TGTGCTCGCCATCCAGCCAGACATGCCCATGAGCAGGCGTCATCAGGCGGCTTAAGGTACGCAGTAACGTGGATTTACCGCAGCCATTTGGCCCGATAATTGCCGTGAAGTGACCATCAGGTATTTCTACAGTCAGATTTTCCGCAACGGTATATTTGCCA  >  NC_000913/619877‑620149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A