Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F48 I1 R1	115	87.5	3003888	99.1%	2976853	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	485,151	C→A	V157L (GTA→TTA)	acrA ←	multidrug efflux pump membrane fusion lipoprotein AcrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	485,151	0	C	A	100.0%	288.5 / NA	80	V157L (GTA→TTA)	acrA	multidrug efflux pump membrane fusion lipoprotein AcrA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (36/44); total (36/44)

CGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGC  >  NC_000913/485014‑485283

cgcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACg                                                                                                                                    <  2:1497566/140‑1 (MQ=255)

 gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACgc                                                                                                                                   >  1:496250/1‑140 (MQ=255)

     tCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCGTTCGCCGCAGTTAACgcagca                                                                                                                               >  1:1118460/1‑140 (MQ=255)

          tGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGc                                                                                                                          >  1:1369770/1‑140 (MQ=255)

           gACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGcc                                                                                                                         <  1:1279902/140‑1 (MQ=255)

             ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTg                                                                                                                       >  2:1113919/1‑140 (MQ=255)

                    gTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCa                                                                                                                <  1:1268327/140‑1 (MQ=255)

                     tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGTGCAt                                                                                                               <  1:890894/140‑1 (MQ=255)

                     tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCAt                                                                                                               <  1:1334298/140‑1 (MQ=255)

                     tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCAt                                                                                                               <  2:1468129/140‑1 (MQ=255)

                         aaTGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGc                                                                                                           <  1:1113919/140‑1 (MQ=255)

                         aaTGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGc                                                                                                           <  2:1320128/140‑1 (MQ=255)

                         aaTGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGc                                                                                                           <  2:1371071/140‑1 (MQ=255)

                          aTGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGcc                                                                                                          >  2:419616/1‑140 (MQ=255)

                          aTGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGcc                                                                                                          >  1:542465/1‑140 (MQ=255)

                           tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCa                                                                                                         >  1:992183/1‑140 (MQ=255)

                           tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCa                                                                                                         >  2:1276360/1‑140 (MQ=255)

                            gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCag                                                                                                        <  1:33049/140‑1 (MQ=255)

                            gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCag                                                                                                        <  1:477322/140‑1 (MQ=255)

                             cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCaga                                                                                                       >  1:724922/1‑140 (MQ=255)

                               ccTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGc                                                                                                     >  1:618904/1‑140 (MQ=255)

                               ccTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGc                                                                                                     <  2:456337/140‑1 (MQ=255)

                               ccTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGc                                                                                                     >  1:353076/1‑140 (MQ=255)

                               ccTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGc                                                                                                     <  2:1061994/140‑1 (MQ=255)

                                cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGcc                                                                                                    <  2:353076/140‑1 (MQ=255)

                                cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGcc                                                                                                    <  1:1077108/140‑1 (MQ=255)

                                cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGcc                                                                                                    <  1:382972/140‑1 (MQ=255)

                                 ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCt                                                                                                   <  1:228661/140‑1 (MQ=255)

                                 ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCt                                                                                                   <  1:876482/140‑1 (MQ=255)

                                        aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTa                                                                                            <  2:257051/140‑1 (MQ=255)

                                          gTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTAct                                                                                          >  1:559777/1‑140 (MQ=255)

                                          gTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTAct                                                                                          <  2:542465/140‑1 (MQ=255)

                                          gTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTAct                                                                                          <  2:1135441/140‑1 (MQ=255)

                                           ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTActc                                                                                         <  1:1131829/140‑1 (MQ=255)

                                            tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTActct                                                                                        >  1:571576/1‑140 (MQ=255)

                                              gACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCttt                                                                                      >  2:929492/1‑139 (MQ=255)

                                              gACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTg                                                                                      <  1:24585/140‑1 (MQ=255)

                                                cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCt                                                                                    <  2:945258/140‑1 (MQ=255)

                                                cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCt                                                                                    <  1:861922/140‑1 (MQ=255)

                                                attACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCt                                                                                    <  2:559777/139‑1 (MQ=255)

                                                  tACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTa                                                                                  <  2:616135/140‑1 (MQ=255)

                                                   aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTAc                                                                                 <  2:618904/140‑1 (MQ=255)

                                                       aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGAt                                                                             <  2:1369770/140‑1 (MQ=255)

                                                       aTGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGAt                                                                             <  1:1113860/140‑1 (MQ=255)

                                                        tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTa                                                                                  >  1:358571/1‑134 (MQ=255)

                                                        tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTa                                                                                  <  2:358571/134‑1 (MQ=255)

                                                        tGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTATTGCTTACTGATg                                                                            >  1:774568/1‑140 (MQ=255)

                                                            actgttAATCTGAGAGGCGCCTTTGGTGTAAGCCAGATTGCTCCGCGCAGTTTCAACGGCAGCTTTCCCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACt                                                                        >  2:1417154/6‑140 (MQ=255)

                                                            aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACt                                                                        <  1:152004/140‑1 (MQ=255)

                                                            aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACt                                                                        >  1:461699/1‑140 (MQ=255)

                                                            aCCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTAGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACt                                                                        >  2:66181/1‑140 (MQ=255)

                                                             ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTg                                                                       >  2:793444/1‑140 (MQ=255)

                                                             ccGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTg                                                                       >  1:496343/1‑140 (MQ=255)

                                                              cGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGa                                                                      >  1:635097/1‑140 (MQ=255)

                                                                 tAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGTCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCCTGCTTACTGATGTACTGAGTa                                                                   <  2:1118460/140‑1 (MQ=255)

                                                                      gtagagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAg                                                              <  2:931628/138‑1 (MQ=255)

                                                                      ggAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAg                                                              <  2:992183/140‑1 (MQ=255)

                                                                      ggAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAg                                                              <  2:461699/140‑1 (MQ=255)

                                                                      ggAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAg                                                              <  2:635097/140‑1 (MQ=255)

                                                                        agagGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCa                                                            >  1:1224134/1‑140 (MQ=255)

                                                                              gACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCt                                                      >  1:561186/1‑140 (MQ=255)

                                                                               aCTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTg                                                     >  1:735740/1‑140 (MQ=255)

                                                                                  ttGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATa                                                  >  1:315671/1‑140 (MQ=255)

                                                                                   tGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATaa                                                 <  2:731820/140‑1 (MQ=255)

                                                                                   tGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATaa                                                 >  2:1195942/1‑140 (MQ=255)

                                                                                    ggTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAAc                                                >  1:985604/1‑140 (MQ=255)

                                                                                         aaGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTc                                           >  1:1493363/1‑140 (MQ=255)

                                                                                               gATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTc                                     >  1:910928/1‑140 (MQ=255)

                                                                                               gATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTc                                     >  1:1181680/1‑140 (MQ=255)

                                                                                                     tCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTgc                               >  2:245387/1‑140 (MQ=255)

                                                                                                     tCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTgc                               >  1:614081/1‑140 (MQ=255)

                                                                                                     tCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTgc                               >  2:942186/1‑140 (MQ=255)

                                                                                                      ccGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTgcg                              >  2:352062/1‑140 (MQ=255)

                                                                                                        gcgcAGTTTCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGcgcg                            <  2:932278/140‑1 (MQ=255)

                                                                                                              tttCAACGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTg                      >  1:414140/1‑140 (MQ=255)

                                                                                                                  aaCGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTg                  <  1:526744/140‑1 (MQ=255)

                                                                                                                  aaCGGCAGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTg                  <  2:1224134/140‑1 (MQ=255)

                                                                                                                        aGCTTTCGCCGCAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCt            >  1:1416590/1‑140 (MQ=255)

                                                                                                                        aGCTTTCGCCGCAGTTAACGCAGCATTAGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCt            <  1:66181/140‑1 (MQ=255)

                                                                                                                                 cgcAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCg   <  1:166700/140‑1 (MQ=255)

                                                                                                                                  gcAGTTAACGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGc  <  2:1372080/140‑1 (MQ=255)

CGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAGCTTTCGCCGCAGTTACCGCAGCATTCGCCTGTTGCGCATCAGCCAGAGCCTGATCGTACTCTTGCTTACTGATGTACTGAGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGC  >  NC_000913/485014‑485283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A