Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F48 I1 R1	83	81.6	2760494	99.1%	2735649	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	485,359	G→T	I87I (ATC→ATA)	acrA ←	multidrug efflux pump membrane fusion lipoprotein AcrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	485,359	0	G	T	99.0%	358.1 / ‑3.8	100	I87I (ATC→ATA)	acrA	multidrug efflux pump membrane fusion lipoprotein AcrA
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (51/48); total (52/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 103 total aligned reads displayed.

AGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACT  >  NC_000913/485222‑485494

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  1:1374370/1‑140 (MQ=255)

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  1:598120/1‑140 (MQ=255)

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  2:1124689/1‑140 (MQ=255)

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  2:1293963/1‑140 (MQ=255)

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  1:142212/1‑140 (MQ=255)

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  1:713319/1‑140 (MQ=255)

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  2:455582/1‑140 (MQ=255)

aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTAt                                                                                                                                       >  1:786091/1‑140 (MQ=255)

    gTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCg                                                                                                                                   <  2:790118/140‑1 (MQ=255)

     tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGc                                                                                                                                  >  1:1037230/1‑140 (MQ=255)

     tttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGc                                                                                                                                  >  1:431304/1‑140 (MQ=255)

         tGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTAcc                                                                                                                              <  2:292241/140‑1 (MQ=255)

                   tCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCCCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGaaa                                                                                                                    >  2:917473/1‑140 (MQ=255)

                     aCCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACGGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAAtt                                                                                                                  >  2:1284115/1‑140 (MQ=255)

                      ccGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTa                                                                                                                 <  1:938597/140‑1 (MQ=255)

                      ccGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTa                                                                                                                 <  1:954060/140‑1 (MQ=255)

                      ccGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTa                                                                                                                 <  2:713319/140‑1 (MQ=255)

                      ccGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTa                                                                                                                 <  1:1093026/140‑1 (MQ=255)

                          cAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCt                                                                                                             >  2:283415/1‑140 (MQ=255)

                          aaaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCt                                                                                                             <  2:703569/139‑1 (MQ=255)

                           aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACGCCTGCTTCTATGTCGCTCCCTTCTTTGAAATTACGCtt                                                                                                            <  1:905724/140‑1 (MQ=255)

                           aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCtt                                                                                                            <  2:431304/140‑1 (MQ=255)

                                   gATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                                                    <  1:449799/140‑1 (MQ=255)

                                   gATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                                                    <  2:117602/140‑1 (MQ=255)

                                    atatTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAAt                                                                                                   <  1:283415/140‑1 (MQ=255)

                                          gCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCt                                                                                             <  2:544158/140‑1 (MQ=255)

                                          gCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCt                                                                                             <  2:1037230/140‑1 (MQ=255)

                                          gCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCt                                                                                             <  1:1062939/140‑1 (MQ=255)

                                          gCTGCAGCCTGGGCTTTCGCCAGATCACATCTCGCACTCTCGTATGTCGCCTGATAGGCCGCAAGATCAATCTGATAGCGAGTGACACCTGCTTCTACGTCGCTACCTTCTTTGCAATTACGATTCAGGATAGCCCCGcc                                                                                             <  2:1297587/140‑2 (MQ=255)

                                               aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGTGAGACACTTGCTTCTATGTCTCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt                                                                                        >  2:410022/1‑140 (MQ=255)

                                               aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt                                                                                        >  1:269745/1‑140 (MQ=255)

                                               aGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt                                                                                        >  1:1317482/1‑140 (MQ=255)

                                               aGCCTGGGCTTTCCCCAGATCACCTTTCTCACTGTCGTATGTTGCCTGATAGGTCTCAGTATCCATCTGATAGTGAGAGTCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCTCTatatt                                                                                        >  2:662222/1‑136 (MQ=255)

                                                 ccTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGa                                                                                      >  1:1351707/1‑140 (MQ=255)

                                                   tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAgg                                                                                    <  1:1057630/140‑1 (MQ=255)

                                                    gggCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGa                                                                                   <  2:1184862/140‑1 (MQ=255)

                                                      gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg                                                                                 <  2:269745/140‑1 (MQ=255)

                                                        tttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTATCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                                               <  1:1231493/140‑1 (MQ=255)

                                                         ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                                              >  2:1329598/1‑140 (MQ=255)

                                                           cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                                                            >  2:1013976/1‑140 (MQ=255)

                                                              cAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                                         <  2:391924/140‑1 (MQ=255)

                                                                  tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGAt                                                                     <  2:969715/140‑1 (MQ=255)

                                                                  tCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGAt                                                                     <  1:114255/140‑1 (MQ=255)

                                                                     ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                                                  <  1:972851/140‑1 (MQ=255)

                                                                      cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                                 >  1:464498/1‑140 (MQ=255)

                                                                        ttCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                                               >  1:372723/1‑140 (MQ=255)

                                                                          cGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                                                             >  2:530814/1‑140 (MQ=255)

                                                                          cGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                                                             <  1:74526/140‑1 (MQ=255)

                                                                           gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc                                                            >  2:744614/1‑140 (MQ=255)

                                                                           gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc                                                            >  2:1060729/1‑140 (MQ=255)

                                                                                gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTgt                                                       >  2:1130407/1‑139 (MQ=255)

                                                                                gTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTgg                                                       >  1:220081/1‑140 (MQ=255)

                                                                                   gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                    >  1:287756/1‑140 (MQ=255)

                                                                                    tATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCg                                                   >  1:517095/1‑140 (MQ=255)

                                                                                     aTGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                                                  >  2:257653/1‑140 (MQ=255)

                                                                                          gCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                                             <  2:786091/140‑1 (MQ=255)

                                                                                           ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                                                     >  1:233061/1‑131 (MQ=255)

                                                                                           ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                                                     <  2:233061/131‑1 (MQ=255)

                                                                                           ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                            >  1:657073/1‑140 (MQ=255)

                                                                                           ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                            >  1:239317/1‑140 (MQ=255)

                                                                                           ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                            >  1:3975/1‑140 (MQ=255)

                                                                                           ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                            >  1:1238849/1‑140 (MQ=255)

                                                                                           ccTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                                            >  1:138236/1‑140 (MQ=255)

                                                                                              gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGCAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg                                          >  1:1134134/1‑139 (MQ=255)

                                                                                              gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg                                          <  2:1134134/139‑1 (MQ=255)

                                                                                              gATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                                         >  1:1209433/1‑140 (MQ=255)

                                                                                               aTAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                        <  1:917473/140‑1 (MQ=255)

                                                                                               aTAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                        >  1:1228497/1‑140 (MQ=255)

                                                                                                  ggTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                     <  1:1076590/140‑1 (MQ=255)

                                                                                                    tCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                   <  2:1238849/140‑1 (MQ=255)

                                                                                                    tCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt                                   <  2:657073/140‑1 (MQ=255)

                                                                                                       caagATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                <  2:86488/137‑1 (MQ=255)

                                                                                                       cAGGATCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                <  1:639437/140‑1 (MQ=255)

                                                                                                            tCAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg                           <  2:1209433/140‑1 (MQ=255)

                                                                                                             cAATCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGc                          <  2:517095/140‑1 (MQ=255)

                                                                                                               aTCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                        <  1:1060729/140‑1 (MQ=255)

                                                                                                               aTCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                        <  2:1362825/140‑1 (MQ=255)

                                                                                                               aTCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                        <  2:531358/140‑1 (MQ=255)

                                                                                                               aTCTGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCAGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag                        <  1:1329598/140‑1 (MQ=255)

                                                                                                                  tGATAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAgg                     >  2:1251597/1‑140 (MQ=255)

                                                                                                                    aTAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGtt                   <  2:464498/140‑1 (MQ=255)

                                                                                                                    aTAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGtt                   >  2:527697/1‑140 (MQ=255)

                                                                                                                     tAGAGAGAGACACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTc                  >  2:199358/1‑140 (MQ=255)

                                                                                                                      agagagagACACCTGCTTCTATGTCGCTACCTTCTTTTAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCa                 >  1:169609/1‑140 (MQ=255)

                                                                                                                        agagagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGt               >  1:380603/1‑140 (MQ=255)

                                                                                                                        agagagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGt               >  1:615566/1‑140 (MQ=255)

                                                                                                                         gagagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtt              <  1:882776/140‑1 (MQ=255)

                                                                                                                          agagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt             >  2:165290/1‑140 (MQ=255)

                                                                                                                          agagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt             >  1:1300865/1‑140 (MQ=255)

                                                                                                                          agagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt             >  2:675705/1‑140 (MQ=255)

                                                                                                                          agagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt             >  2:843433/1‑140 (MQ=255)

                                                                                                                          agagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt             >  1:757754/1‑140 (MQ=255)

                                                                                                                           gagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtttt            <  2:757754/140‑1 (MQ=255)

                                                                                                                           gagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtttt            <  1:74323/140‑1 (MQ=255)

                                                                                                                           gagaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtttt            <  2:1013803/140‑1 (MQ=255)

                                                                                                                             gaCACCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGa          <  1:1124689/140‑1 (MQ=255)

                                                                                                                              acacCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGAc         >  2:1364729/1‑140 (MQ=255)

                                                                                                                              acacCTGCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGAc         <  2:287756/140‑1 (MQ=255)

                                                                                                                               cgcttGCATCTATGTCGCTACCTTCTTTGAAATTGCGCGCCAGGATAATCCCGCGAACTTGAGGACGAGTTTCTGTGATCCGGTAGGCACTGGTGCTACTCGGAAGCTTGGTTGTGAACTGCATAGGTTCAGTTTGGACt        <  2:21024/136‑1 (MQ=255)

                                                                                                                                    gCTTCTATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTtac   >  2:306720/1‑140 (MQ=255)

AGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACT  >  NC_000913/485222‑485494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A